Embryo vitrification thawing method and embryo verification thawing vessel

Abstract

The invention discloses an embryo vitrification thawing vessel. The embryo vitrification thawing vessel comprises a vessel bottom and a vessel cover which are matched with each other, as well as an embryo transfer device and an embryo limiting ring which is made of an elastic material, wherein the vessel bottom is formed by combination of a vessel bottom I and a vessel bottom II which are equal in height; a hollow thawing cavity is formed in the vessel bottom I, and the embryo limiting ring is arranged at the top of the thawing cavity; a diluting cavity and a washing cavity are formed in the vessel bottom II, and an embryo transfer device channel is formed between the vessel bottom and the vessel cover, comprises an embryo net bag, and can move along the embryo transfer device channel so as to drive embryos in the embryo net bag to sequentially enter the diluting cavity and the washing cavity from the thawing cavity; therefore, the thawed embryos can be transferred among different liquids. The invention further provides a method for using the embryo vitrification thawing vessel.

Description

technical field [0001] The invention relates to the technical field of reproductive medicine, medical treatment and health, in particular to an embryo vitrification thawing dish and a use method thereof. Background technique [0002] Embryo vitrification and thawing are routine operations in human assisted reproductive technology laboratories, which allow patients to have multiple embryo transfer opportunities in one IVF-ET (in vitro fertilization-embryo transfer, ie, test-tube baby) treatment cycle, The success rate of treatment is greatly improved, and the quality of embryo vitrification and thawing is directly related to the final treatment outcome. Embryo vitrification and thawing is one of the focuses in the field of IVF in recent years. Compared with the slow programmed freezing and thawing that has been used for more than 20 years, it is faster, simpler, and has a higher freeze-thaw recovery rate. The freeze-thaw recovery rate is higher than 90%. %. Embryo vitrifica...

AI Technical Summary

Problems solved by technology

Embryo vitrification and thawing have been adopted by more and more reproductive center laboratories in recent years, and there are commercial embryo vitrification and thawing solutions available for purchase, but there is no corresponding embryo vitrification and thawing container. Petri dishes and four-hole dishes are used as containers for the thawing solution, and a thin capillary glass tube is used to transfer embryos. However, when vitrified embryos are transferred from liquid nitrogen to the thawing solution, the embryos will float on the surface of the thawing solution, making it difficult to observe Embryos sink to the bottom in diluents and washes and may be lost during transfer

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