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Composition for culturing epithelial cells and use thereof in maintaining epithelioid cell morphology

A technology of epithelial cells and cell morphology, which is applied in the field of epithelial cell culture compositions, and can solve problems such as changes in fibrous morphology

Inactive Publication Date: 2017-06-30
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, RPE cells cultured and subcultured in vitro are prone to morphological changes, leading to epithelial-mesenchymal transition (Epithelial-Mesenchymal Transition, EMT), and finally forming fibrous morphological changes

Method used

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  • Composition for culturing epithelial cells and use thereof in maintaining epithelioid cell morphology
  • Composition for culturing epithelial cells and use thereof in maintaining epithelioid cell morphology
  • Composition for culturing epithelial cells and use thereof in maintaining epithelioid cell morphology

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Embodiment Construction

[0021] The technical solution of the present invention will be described in detail below in conjunction with the accompanying drawings. The embodiments of the present invention are only used to illustrate the technical solutions of the present invention without limitation. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the invention can be modified or equivalently replaced , without departing from the spirit and scope of the technical solution of the present invention, all of which shall be covered by the claims of the present invention.

[0022] The isolation and culture methods of RPE cells are as follows:

[0023] Eyeballs from three female donors with an average age of 45 years. Consent letters have been obtained from the family members of each donor and approved by the Ethics Committee of Jinan University. At the same time, all human-relat...

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Abstract

A composition for culturing epithelial cells includes matrix gel (Matrigel) and activin A (ActivinA). The composition is suitable for in-vitro cultivation of the epithelial cells, especially suitable for in-vitro cultivation of retinal pigment epithelium ( RPE) cells, the composition maintains epithelioid cell morphology, membrane polarity and barrier functions, normal cell phenotype and phagocytosis.

Description

technical field [0001] The invention relates to a cell culture composition, in particular to a composition for culturing epithelial cells, which keeps the epithelial cells in the form of epithelial cells during in vitro culture to facilitate cell transplantation. Background technique [0002] The main function of epithelial cells is to form a dynamic barrier to regulate the transmission of substances and signals between cells in the tissue. [0003] Retinal Pigment Epithelium (RPE cells) is a single layer of cells located between the choroid and the retinal nerve layer. It plays an important role in maintaining the normal function of photoreceptor cells and the retina, including: absorbing stray light, forming the choroid The barrier between the retina and the retina, produces visual pigments, provides nutrients to the neural retina, and phagocytizes the outer segments of photoreceptor cells that are shed (Physiol. Rev. 2005, 85, 845-881). The degradation or damage of RPE c...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0621C12N2501/16C12N2533/90
Inventor 郭晓令葛仁山陈建苏连瑞玲朱德良李超李晓珩王秀娣陈勇陈显武段越
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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