Method for preparing mussel protein and polypeptide with different dissolubilities

A soluble and mussel technology, applied in the field of mussel protein and polypeptide preparation, can solve the problems of low protein extraction rate, unclear step-by-step extraction technology of three soluble proteins, etc., achieve broad market prospects and improve utilization rate Effect

Inactive Publication Date: 2017-07-07
DALIAN POLYTECHNIC UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0009] (1) The step-by-step extraction techniques for the three soluble proteins are unclear;
[0010] (2) The extraction rate of various proteins is low;
[0011] (3) The prepared protein has not been processed and developed into a product that can be directly applied
Researches on protein grading are mostly concentrated on wheat, rice, buckwheat and other crop protein sources, but there are few reports on the grading and extraction process of mussel protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of embodiment 1 mussel protein:

[0030] 1) Pre-treatment: cleaning, shelling, silk removal, homogenization 5000-8000rpm / min, time 15-30min.

[0031] 2) The sarcoplasmic protein extract is 0.05mol / L, pH=7 phosphate buffer solution, the extraction time is 30-90min, the number of extractions is 2-3 times, the temperature is 2-4°C, and the ratio of solid to liquid is 1:3-1 : 20. Centrifugal conditions: 8000-13000g, 10-20min, 4°C. The final protein solution was ultrafiltered 2-6 times with a 10000Da membrane.

[0032] 3) Myofibrillar protein extract is 0.1mol / L, pH=7 phosphate buffer solution containing 0.6mol / L sodium chloride; extraction time 30-60min; extraction times 2-3 times; temperature 2-4℃ ; Ratio of solid to liquid: 1:3-1:20; Centrifugal conditions: 8000-13000g, 10-20min, 4°C. The final protein solution was ultrafiltered 2-6 times with a 10000Da membrane.

[0033] 4) The matrix protein extract is 2%-5% citric acid solution, the extractio...

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Abstract

The invention discloses a method for preparing mussel proteins and polypeptide with different dissolubilities. The method comprises the following steps: cleaning, removing shells, removing byssi, and performing homogenization; sequentially extracting myogen, fibrillin and a matrix protein so as to obtain corresponding extracts, performing ultrafiltration respectively, and finally performing spray drying, thereby obtaining protein powder. In addition, the protein extracts are subjected to enzymolysis, enzyme deactivation, centrifugation to obtain supernate, and the supernate is subjected to ultrafiltration and spray drying, thereby obtaining polypeptide powder. According to different dissolubilities, mussel is divided into the myogen, the fibrillin and the matrix protein. By virtue of the characteristics that the myogen is soluble in a water solution or a thin salt solution, the fibrillin is soluble in a salt solution of certain concentration and the matrix protein is insoluble in water, mixed proteins in the mussel are appropriately separated. In addition, the proteins are adopted for enzymolysis respectively, then three different enzymolysis polypeptide products can be prepared, and the utilization rates of raw materials are increased.

Description

technical field [0001] The invention relates to a preparation method of mussel proteins and polypeptides with different solubility. Background technique [0002] Mussels are an important species in the shellfish farming business and are produced in large quantities. The protein content of dried mussels is about 60%, with high economic value and certain practical value. In order to fully develop and utilize mussels, the present invention adopts a step-by-step extraction method to dissolve the protein of the raw material, and at the same time better separate the mixed protein in the raw material according to the difference in protein solubility, so as to facilitate the later analysis of different proteins. research. [0003] In the current research, there is no exact report on the composition and content of mussel protein, and the amino acid sequence of various components in mussel protein is rarely reported. This has certain limitations for the in-depth study of mussel pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/34C07K1/14C12P21/06
CPCC07K14/43509C12P21/06
Inventor 杜明乔美玲陈慧樊凤娇石璞洁王震宇徐献兵
Owner DALIAN POLYTECHNIC UNIVERSITY
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