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Site-directed mutated influenza virus, and live vaccine, preparation method and application thereof

A technology of influenza virus and site-directed mutation, applied in the field of influenza virus, application of influenza virus, site-directed mutation of influenza virus, live vaccine of influenza virus, can solve problems such as interference, and achieve the effect of increasing safety

Inactive Publication Date: 2017-07-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cold-adapted live attenuated vaccines can reassort with other influenza viruses to obtain virulent reassortant viruses, and interference may occur in bivalent or trivalent cold-adapted live attenuated vaccines

Method used

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  • Site-directed mutated influenza virus, and live vaccine, preparation method and application thereof
  • Site-directed mutated influenza virus, and live vaccine, preparation method and application thereof
  • Site-directed mutated influenza virus, and live vaccine, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 7

[0301] Changes in the content of NP-specific CD8+T cells in the mouse lung after embodiment 7WSN-RNP-tag immunization

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PUM

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Abstract

The invention relates to an influenza virus subjected to site-directed mutation or an influenza virus with simultaneous mutation of multiple sites. The influenza virus can be derived from human and other animals. The invention further relates to a method of the site-directed mutated influenza virus. The method includes introducing UAG into an influenza virus genome according to a reverse inheritance technology and introducing unnatural amino acid into an influenza virus gene in a site-directed way according to a gene codon extension technology. The invention further relates to application of the site-directed mutated influenza virus or the influenza virus with multiple mutation site combinations, such as serving as attenuated live vaccines and copying controllable safe influenza virus models.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and specifically relates to a live influenza virus vaccine with site-directed mutation, site-directed modification, and controllable replication. The sites of site-directed mutations are combined to prepare influenza viruses that introduce unnatural amino acids into multiple gene segments. The present invention also relates to a method for site-directed mutagenesis of influenza virus, the method comprising using gene codon expansion technology and gene codon expansion technology to site-directly introduce unnatural amino acid into any gene of influenza virus. The present invention further relates to the application of the influenza virus with site-directed mutation or multi-point combination, such as the use as a live vaccine of influenza virus, a safety model of virulence of influenza virus, and the like. Background technique [0002] Influenza is an acute respiratory disease caused by influen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C07K14/11C12N15/44C12N15/63A61K39/145A61K35/76A61P31/16C12N5/10
CPCA61K35/76C07K14/005C12N7/00C12N9/93C12N2760/16034C12N2760/16022C12N2760/16021C12N5/10C12N15/63A61K39/12C12N2760/16134C12N2760/16162A61P31/16C12Y601/01026
Inventor 周德敏司龙龙周雪莹张子威徐欢田振宇姚天卓肖苏龙张传领夏青张礼和
Owner PEKING UNIV
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