Site-directed mutated influenza virus, and live vaccine, preparation method and application thereof

Abstract

The invention relates to an influenza virus subjected to site-directed mutation or an influenza virus with simultaneous mutation of multiple sites. The influenza virus can be derived from human and other animals. The invention further relates to a method of the site-directed mutated influenza virus. The method includes introducing UAG into an influenza virus genome according to a reverse inheritance technology and introducing unnatural amino acid into an influenza virus gene in a site-directed way according to a gene codon extension technology. The invention further relates to application of the site-directed mutated influenza virus or the influenza virus with multiple mutation site combinations, such as serving as attenuated live vaccines and copying controllable safe influenza virus models.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and specifically relates to a live influenza virus vaccine with site-directed mutation, site-directed modification, and controllable replication. The sites of site-directed mutations are combined to prepare influenza viruses that introduce unnatural amino acids into multiple gene segments. The present invention also relates to a method for site-directed mutagenesis of influenza virus, the method comprising using gene codon expansion technology and gene codon expansion technology to site-directly introduce unnatural amino acid into any gene of influenza virus. The present invention further relates to the application of the influenza virus with site-directed mutation or multi-point combination, such as the use as a live vaccine of influenza virus, a safety model of virulence of influenza virus, and the like. Background technique [0002] Influenza is an acute respiratory disease caused by influen...

AI Technical Summary

Problems solved by technology

However, cold-adapted live attenuated vaccines can reassort with other influenza viruses to obtain virulent reassortant viruses, and interference may occur in bivalent or trivalent cold-adapted live attenuated vaccines

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