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Method for detecting achyranthes root

A detection method, the technology of Achyranthes bidentata, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of safety, effectiveness and quality control of unfavorable clinical application of Achyranthes bidentata, achieve good stability and meet the requirements of quantitative detection Effect

Inactive Publication Date: 2017-07-14
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Achyranthes bidentata is a commonly used clinical medicinal material in Yi medicine in Liangshan Yi Autonomous Prefecture of Sichuan, Chuxiong Yi Autonomous Prefecture of Yunnan, and Guichuan. At present, the quality standards for medicinal materials and prescriptions have not been established, which is extremely detrimental to the safety and security of clinical application of Achyranthes bidentata. effective and quality controlled

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  • Method for detecting achyranthes root
  • Method for detecting achyranthes root
  • Method for detecting achyranthes root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] In order to further clarify the chemical components and active ingredients in the Yi medicine Achyranthes bidentata, obtain reference substances, establish the quality standards of the medicinal material Achyranthes bidentata and its prescription preparations, and clarify the material basis of the pharmacological effects of Achyranthes bidentata, this example studies the medicinal materials of Achyranthes bidentata. The chemical composition has been studied systematically.

[0052] 1 Instruments and materials

[0053] 1.1 Instrument

[0054] Waters Acquity UPLC H-Class System ultra-high performance liquid chromatography (Waters, USA); METTLER AE240 electronic analytical balance (Mettler-Toledo (Shanghai) Instrument Co., Ltd.); KQ-250B ultrasonic cleaner (Kunshan City) Ultrasonic Instrument Co., Ltd.); IKA RV10 rotary evaporator (German IKA company); SHB-Ⅲ circulating water vacuum pump (Gongyi Yingyu Huake Instrument Factory); digital constant temperature water bath HH-...

Embodiment 2

[0082] Instruments and materials

[0083] 1.1 Instrument

[0084] Waters Acquity UPLC H-CLass System ultra-high performance liquid chromatography (Waters, USA); METTLER AE240 electronic analytical balance (Mettler-Toledo (Shanghai) Instrument Co., Ltd.); IKA RV10 rotary evaporator (IKA, Germany) ; KQ-250B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); SHB-Ⅲ circulating water vacuum pump (Gongyi Yingyu Huake Instrument Factory); digital constant temperature water bath HH-2 (Guohua Electric Co., Ltd.).

[0085] 1.2 Materials and reagents

[0086] The source of medicinal materials is shown in Table 4. It was identified by Professor Liu Yuan of Southwest University for Nationalities as the dry root of CyatHulaCapitata (Wall.) Moq., a plant of the family Amaranthaceae. -O-[α-L-rhamnopyranose (1→3)-β-D-glucopyranose]-oleanolic acid (HNX-3 for short) and 3-O-[α-L- Rhamnopyranose (1→3)-β-D-glucopyranose]-oleanolic acid-28-O-(β-D-glucopyranose) (referred to as HNX-5),...

Embodiment 3

[0123] Embodiment 3 TLC identification

[0124] 1 Preparation of reference solution

[0125] Take an appropriate amount of the reference substance HNX-5, add methanol to make a solution containing 2 mg per 1 mL, and use it as the reference substance solution.

[0126] 2 Preparation of the test solution

[0127] Take 1g of the powder of this product, and extract the sample according to the optimized method under item 4.3 in the text, that is, add 20mL of 80% ethanol (v / v), heat to reflux for 1h, filter, concentrate the filtrate until it has no alcohol smell, add 30mL of water, petroleum ether (30mL ) after degreasing, extracted with n-butanol (30mL×2), concentrated under reduced pressure to remove n-butanol, and added 2mL of methanol to dissolve the residue. As the test solution.

[0128] 3 TLC condition investigation

[0129] 3.1 Expansion method

[0130] Take 5 μL each of the reference substance and the sample, and spot on the same silica gel G plate (200mm×100mm), and e...

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Abstract

The invention provides a method for detecting achyranthes root. The method utilizes a UPLC detection method. The method uses 3-O-[alpha-L-rhamnopyranosyl(1-to-3)-beta-D-pyrano-glucuronic acid]-oleanolic acid-28-O-(beta-D-glucopyranose) as an index component for detection of achyranthes root. The UPLC is used for detecting the index component in achyranthes root. The method has good stability and reproducibility, can effectively separate the index component and other components, realizes qualitative research of the index component and satisfies quantitative detection requirements.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicinal materials, in particular to a detection method of Achyranthes bidentata. Background technique [0002] Red Achyranthes bidentata is a commonly used clinical medicinal material in Yi medicine in Liangshan Yi Autonomous Prefecture of Sichuan, Chuxiong Yi Autonomous Prefecture of Yunnan, Guizhou and other Yi ethnic areas. It is the root of Cyathula Capitata (Wall.) Moq., a plant of the family Amaranthaceae, and is used to treat bruises, rheumatism, rheumatoid and other diseases. [0003] The domestic and foreign research on Achyranthes bidentata mainly focuses on Achyranthesbidentata Blume and Cyathula officinalis Kuan, which are commonly used in TCM clinical practice; there are few related studies on Achyranthes bidentata. Achyranthes bidentata is a commonly used clinical medicinal material in Yi medicine in Liangshan Yi Autonomous Prefecture of Sichuan, Chuxiong Y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/90
CPCG01N30/02G01N30/90
Inventor 刘圆王景富王静霞李莹
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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