Method for extracting hyaluronidase from bovine spleen

A technology of hyaluronidase and spleen, which is applied in the biological field, can solve the problem of extracting less hyaluronidase, and achieve the effect of being suitable for large-scale production, simple purification steps, and increasing extraction rate
CN106967700AInactive Publication Date: 2017-07-21NANNING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANNING UNIV
Publication Date
2017-07-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for extracting hyaluronidase from a bovine spleen. The method comprises the following steps: soaking the bovine spleen in normal saline with a certain concentration, adding extracting liquid to extract, triturate and extract, centrifuging and taking supernatant, adding sodium chloride to remove impurities, adding salt to perform salting-out, and performing dialysis to prepare the refined hyaluronidase. The method is used for extracting the hyaluronidase from the bovine spleen, simplifies the steps, is easy to operate, is high in extraction rate, purity and enzyme activity of the hyaluronidase, and explores a new approach for extraction of the hyaluronidase.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for extracting hyaluronidase from bovine spleen.

[0002] technical background

[0003] Hyaluronidase (Hyaluronidase, HAase) is a mucopolysaccharide endonuclease that can reduce the activity of hyaluronic acid in the body and improve the liquid permeability of tissues. play an important role in. Hyaluronidase is widely found in mammals, invertebrates (crustaceans, insects, leeches), pathogenic bacteria (Candida, Streptomyce), bacteria and phages, and comes from different biological tissues A large number of hyaluronidases have been identified. HAase is widely used in the field of medicine. Its main function is to reduce the relative molecular weight of hydrolyzed hyaluronic acid (HA) while eliminating the viscosity of HA and enhancing the permeability of the body to foreign substances. In medicine, HAase can promote the diffusion of drugs in the body. Therefore, the import...

Claims

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