A kind of extraction method of succinic acid and propionic acid

An extraction method and technology for succinic acid, which are applied in the extraction of succinic acid and propionic acid, and the field of extraction of succinic acid and propionic acid, can solve the problems of containing, lacking downstream extraction processes, and being unable to simultaneously separate and extract succinic acid.

Inactive Publication Date: 2020-10-16
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Nevertheless, the content of calcium propionate in the market is only 40-50% at present. The main reason is that the product contains a large amount of impurities such as succinic acid, lactic acid and acetic acid due to the lack of effective downstream extraction process.
"Journal of Process Engineering, 2016, 16(3): 86-91", researcher Wang Yunshan of the Institute of Process Engineering, Chinese Academy of Sciences, proposed to use ion exchange chromatography to extract propionic acid in the fermentation broth, and initially established its extraction process, which effectively improved the product. Propionic acid content, but it cannot separate and extract succinic acid at the same time, resulting in succinic acid contained in propionic acid, and the content of propionic acid is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The extraction method of described extraction succinic acid and propionic acid comprises the steps:

[0065](1) Strain activation: Inoculate the preserved Propionibacterium in the primary seed medium for static culture, and then transfer to the secondary shake flask seed medium at 30°C for static culture for 48 hours according to the inoculum size of 10%. The seed medium comprises the following components: 35g / L glucose, 21g / L corn steep liquor, 5g / L ammonium sulfate and 4g / L potassium dihydrogen phosphate, and the pH of the seed medium is 6.8-7;

[0066] (2) Fermentation: transfer the activated Propionibacterium to the fermentation medium at 30°C and 50r / min according to the inoculum size of 10%, and carry out fermentation culture. During the fermentation process, when the glucose concentration is lower than 10g / L, add Glucose, adjust pH with ammoniacal liquor to be 6.8-7.2 simultaneously, described fermentation medium comprises following component: 60g / L glucose, 41g / ...

Embodiment 2

[0075] Compared with Example 1, except that the sample loading speed of step (4) and step (5) is 1BV / h, other conditions are the same as Example 1.

[0076] Although the processing capacity of the anion resin remains unchanged, the succinic acid concentration after the elution is 90.24g / L, and the propionic acid concentration after the elution is 93.14g / L; but the loading speed slows down, which increases the operating time.

Embodiment 3

[0078] Compared with Example 1, except that the sample loading speed of step (4) and step (5) is 5BV / h, other conditions are the same as Example 1.

[0079] The throughput of the anion resin decreased, the concentration of succinic acid after elution was 78.52g / L, and the concentration of propionic acid after elution was 80.17g / L.

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Abstract

The invention relates to a method for extracting succinic acid and propionic acid. The method comprises the following steps: (1) pretreating a fermentation liquid: collecting the fermentation solution for acidification; (2) adsorbing the succinic acid: excessively applying the fermentation solution acidified in the step (1) to a first chromatographic unit filled with anion exchange resin, and collecting a column passing solution; (3) adsorbing the propionic acid: applying the column passing solution collected in the step (2) to a second chromatographic unit filled with the anion exchange resin; (4) eluting: eluting the first chromatographic unit and the second chromatographic unit by using alkali and acid to obtain the succinic acid and the propionic acid, wherein in the step (2), excessive application refers to the amount larger than the saturated adsorption amount of the succinic acid relative to the anion exchange resin. According to the method, by a two-stage chromatographic technology, the succinic acid in the fermentation solution is separated, so that coproduction of the succinic acid (succinate) and propionic acid (propionate) is achieved, and the purity of the propionic acid (propionate) in a product is further improved.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to a method for extracting succinic acid and propionic acid, in particular to a method for extracting succinic acid and propionic acid from fermentation liquid. Background technique [0002] Propionic acid and its salts such as calcium propionate, zinc propionate, and potassium propionate can effectively inhibit mold and aerobic bacillus, and are basically harmless to the human body. They are widely used in grains, feed and food, and are good antiseptic and fresh-keeping products. agent. At present, the industrial production method of propionic acid is mainly chemical synthesis method, but in the current situation that the whole world is facing environmental pollution and energy shortage, the production of propionic acid by microbial fermentation has attracted widespread attention. In particular, calcium propionate produced by biological method, referred to as calcium propionate by biolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/46C12P7/52C07C51/47C07C53/122C07C55/10C12R1/01
CPCC07C51/47C12P7/46C12P7/52C07C53/122C07C55/10
Inventor 王自强李小连刘千千王云山苏志国
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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