Method for rapidly identifying H. villosa chromosomes in wheat background

A tufted wheat and chromosome technology, applied in the field of molecular cytogenetics, can solve the problems of inability to identify rye chromosomes, complicated processes, and inability to be clear at a glance, and achieves the effects of overcoming cumbersome preparation, low cost and easy operation.

Active Publication Date: 2017-07-21
SICHUAN AGRI UNIV
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this technology has the following disadvantages: (1) The C-belt technology process is long and complicated
(2) In the process of using the C-banding technique, the wheat chromosomes also have relatively rich banding, which makes it difficult to identify the chromosomes of Trichosanthemi villosa in the background of wheat, and cannot achieve a clear effect
Currently using oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 and ND-FISH to identify rye chromosomes in wheat background can only achieve the effect of GISH technology, that is, only the rye chromosomes in wheat background can be identified. The rye chromosome is distinguished from the wheat chromosome, but it is not possible to identify which rye chromosome

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly identifying H. villosa chromosomes in wheat background
  • Method for rapidly identifying H. villosa chromosomes in wheat background

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Analysis of Wheat-Viligal repeats and Determination of Oligonucleotide Probe Sequences

[0049] According to the characteristics of the distribution of transposable repeat sequences in the plant genome in a scatter manner, we start with the analysis of transposable repeat sequences and search for oligonucleotide probes. Based on the fact that there are relatively few transposable repetitive sequences isolated and cloned from the genome of T. villosa, and based on the principle that each species of wheat crops has a certain genetic relationship, consider using the method of sequence transfer between species to develop suitable oligonucleotides acid probe.

[0050] 1. Determination of oligonucleotide probe nucleotide sequence and probe design

[0051] (1) Collect all the reported transposable repeat sequences isolated and cloned from the rye genome and the reported rye-specific oligonucleotide probe sequences.

[0052] From the NCBI database, download all publ...

Embodiment 2

[0060] Example 2 Establishment of the Chromosomal Identification Method for T. villosa in the Wheat Background

[0061] Two kinds of oligonucleotide probes (Oligo-Ku and (GT) determined in embodiment 1 7 ) were mixed, and ND-FISH was used to identify the chromosomes of A. villosa in the wheat background. The method described in (Han et al.2006) was used for the preparation of the root tip metaphase chromosomes of wheat-T. villosa amphidiploid and wheat cultivar Mianmai 37, and the method described in (Fu et al.2015) for the ND-FISH analysis process . details as follows:

[0062] (1) Preparation of root tip metaphase chromosomes

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for rapidly identifying H. villosa chromosomes in a wheat background. According to the method, two oligonucleotide probes are firstly provided, the nucleotide sequences of the oligonucleotide probes are respectively shown as SEQ ID NO.1-2, the two probes are mixed and combine with an ND-FISH technology and can be used for identifying the H. villosa chromosomes in a wheat and haynaldia villosa hybridization generation, 7 H. villosa chromosomes are accurately identified while wheat chromosomes and the H. villosa chromosomes can be clearly distinguished, and wheat-haynaldia villosa translocated chromosomes can be identified. By adopting the method, the shortcomings of a GISH technology and an existing ND-FISH technology can be overcome, and the effects of the GISH technology and the existing ND-FISH technology can be also achieved. The method facilitates identification of the wheat-haynaldia villosa translocated chromosomes and has the very good prospect in the field of wheat breeding.

Description

technical field [0001] The invention relates to the field of molecular cytogenetics, in particular to a method for using oligonucleotide probes and ND-FISH methods to detect the chromosomes of Trichosanthes villosa in the background of wheat and the translocation chromosomes of wheat- Trismus vistifolia. Background technique [0002] The chromosome sequences of higher organisms are complex and diverse, and there are a large number of repetitive sequences in different intervals, which provides new ideas and methods for marking and identifying chromosomes. In the field of molecular cytogenetics, chromosomes can be marked by constructing repetitive sequence probes, and the distribution of probe signals on chromosomes can be analyzed to distinguish genomes from chromosome groups. In addition, it is a common practice to analyze the behavior of chromosomes by comparing the changes of probe signals on chromosomes. [0003] Trichophyllum villosa is a close relative of wheat, which ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6841C12Q1/6895C12Q2600/166C12Q2563/107
Inventor 符书兰唐宗祥肖志强
Owner SICHUAN AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap