Recombined ternary fused collagen and application thereof

A technology of collagen and human-like collagen, which is applied in the direction of fusion polypeptide, drug combination, peptide/protein components, etc., can solve the problems that collagen is easy to carry viruses, infection, and endanger health, so as to promote human metabolism, fast absorption, Promotes wound healing and tissue repair effects

Inactive Publication Date: 2017-07-28
湖南萨可生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current mature process of obtaining collagen is to use animal tissue extraction. Collagen extracted from mammalian bovine tissue and bird animal chicken tissue is prone to carry viruses, and then infects and endangers human health.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] Wherein, in the above-mentioned recombinant recombinant 3M fusion collagen, the recombinant human-like collagen is prepared by the following method:

[0028] 1) Construction of recombinant human-like collagen engineering bacteria;

[0029] 2) Fermentation and cultivation of engineering bacteria;

[0030] 3) Induction and expression of recombinant human-like collagen;

[0031] 4) Purification of recombinant human-like collagen.

[0032] Wherein, in step 1) of the method for recombinant human-like collagen, the engineering bacteria of recombinant human-like collagen are constructed as follows:

[0033] a. Extract the DNA fragment encoding human-like collagen, modify it with the sequence encoding 3GF growth factor, repeat it specifically, then connect it, and recombine the DNA;

[0034] b. Transforming the above recombinant DNA into Escherichia coli.

[0035] Wherein, in step 2) of the method for recombinant human-like collagen, the fermentation medium of the engineeri...

Embodiment 1

[0046] Recombinant 3M fusion collagen includes recombinant human-like collagen and a new type of lactobionic acid with a mass ratio of 30%; the recombinant human-like collagen is formed by fusing human-like collagen with 3GF growth factor, which includes human-like collagen at least A first region with 80% sequence homology and a second region with at least 80% sequence homology with each human cell growth factor, and a connecting peptide is arranged between the first region and the second region; the general connection peptide The formula is (GGSS) n , n=3. The 3GF growth factors are epidermal growth factor EGF, insulin growth factor IGF and fibroblast growth factor FGF; the main components of the novel lactobionic acid are gluconolactone and lactobionic acid, in parts by weight, gluconic acid 7 parts fat, 80 parts lactobionic acid.

[0047] The recombinant human-like collagen is prepared by the following method:

[0048] 1) Construction of engineering bacteria for recombi...

Embodiment 2

[0054] Recombinant 3M fusion collagen includes recombinant human-like collagen and a new type of lactobionic acid with a mass ratio of 30%; the recombinant human-like collagen is formed by fusing human-like collagen with 3GF growth factor, which includes human-like collagen at least A first region with 80% sequence homology and a second region with at least 80% sequence homology with each human cell growth factor, and a connecting peptide is arranged between the first region and the second region; the general connection peptide The formula is (GGSS) n , n=3. The 3GF growth factors are epidermal growth factor EGF, insulin growth factor IGF and fibroblast growth factor FGF; the main components of the novel lactobionic acid are gluconolactone and lactobionic acid, in parts by weight, gluconic acid 10 parts fat, 180 parts lactobionic acid.

[0055] The recombinant human-like collagen is prepared by the following method:

[0056] 1) Construction of engineering bacteria for recom...

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PUM

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Abstract

The invention discloses recombinant ternary fusion collagen and its application, belonging to the field of genetic engineering. The recombinant ternary fusion collagen includes recombinant human-like collagen and new lactobionic acid with a mass ratio of 25-35%; the recombinant human-like collagen is formed by fusing human-like collagen with 3GF growth factor, which includes A first region with at least 80% sequence homology of human-like collagen and a second region with at least 80% sequence homology with each human cell growth factor, and a connecting peptide is arranged between the first region and the second region. Compared with the traditional human-like collagen, it is easier to be absorbed by the human body. The fusion collagen provided by the present invention is absorbed faster and more thoroughly. At the same time, it can also promote human metabolism, wound healing and tissue repair, yellowing, skin rejuvenation, and improvement Melasma, freckles and other pigmentation, wrinkles and skin tone effect, is a breakthrough new fusion collagen with comprehensive effects.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and specifically relates to a fusion collagen, more specifically, to a recombinant ternary fusion collagen and its application. Background technique [0002] Collagen is a biological polymer substance that plays a role in bonding tissues in animal cells. It is one of the most critical raw materials in the biotechnology industry and is also the best biomedical material with a huge demand. It is widely used in biomedicine Materials, cosmetics and food engineering and other fields. The collagen sponge loaded with growth factors has the functions of tissue repair, wound healing promotion, yellow dispelling and skin rejuvenation. However, the current mature technology for obtaining collagen is to extract it from animal tissue. Collagen extracted from mammalian bovine tissue and bird animal chicken tissue is prone to carry viruses, which in turn infects and endangers human health. [0003] Lactobio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/70A61L27/24A61L27/54A61K38/39A61K38/30A61K38/18A61K8/65A61K8/64A61P17/02A61P17/00A61P17/18A61Q19/02A61Q19/08
CPCC07K14/78A61K8/64A61K8/65A61K38/00A61L27/24A61L27/54A61L2300/412C07K14/475C07K14/485C07K14/65C07K2319/00C12N15/62C12N15/70
Inventor 刘勇
Owner 湖南萨可生物医药有限公司
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