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Culture method of petroleum degrading bacteria

A technology of petroleum-degrading bacteria and culturing method, which is applied in the field of culturing petroleum-degrading bacteria, can solve problems such as shortage, and achieve the effects of good low temperature resistance and good salt resistance.

Inactive Publication Date: 2017-08-18
XIAN HUANUO ENVIRONMENTAL PROTECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of oilfield sewage treatment, microbial technology is widely used, but the biggest problem is the lack of functional bacteria that can degrade oil efficiently with crude oil as the substrate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 100mL of simulated oily sewage with a salinity of 6500mg / L, a pH of 6.0, and an oil content of 200mg / L in four 150mL Erlenmeyer flasks, numbered A, B, C, and D, and pour them into three flasks A, B, and C. Insert 0.2mL of purified oil-removing bacteria solution into the Erlenmeyer flask, D as a blank control, 25, cultured for 24h respectively, based on the D blank, measure the content with a UV spectrophotometer, and calculate the oily degradation rate, the result As shown in the following table:

[0024] Numbering Culture temperature / (℃) Oil degradation rate / (%) A 15 81.2 B 25 89.5 C 35 86.8

Embodiment 2

[0026] Take 100mL of simulated oily sewage with a salinity of 6500mg / L, an oil content of 200mg / L, and a pH configured according to the table below, and place them in five 150mL conical flasks, numbered A, B, C, D, E, and pour them into A and B Insert 0.2mL of purified oil-removing bacteria solution into three Erlenmeyer flasks, C, D, and E as a blank control. Under the condition of 25°C, culture them for 24 hours respectively. Based on the blank of E, measure with an ultraviolet spectrophotometer With content, calculate the oil-containing degradation rate, the results are as follows:

[0027] Numbering pH Oil degradation rate / (%) A 5.0 77.6 B 6.0 81.8 C 7.0 82.9 D 8.0 79.5

Embodiment 3

[0029] Take 100mL of oily sewage with an oil content of 200mg / L, pH7.0, and salinity set as shown in the table below, and place them in four 150mL conical flasks, numbered A, B, C, and D, and pour them into three flasks A, B, and C. Insert 0.2mL of the bacteria solution of high-efficiency oil-removing bacteria into the Erlenmeyer flask, and D was used as a blank control. Under the condition of 25°C, cultured for 24 hours respectively. Based on the blank of D, the content was measured with a UV spectrophotometer, and the oil-containing degradation rate was calculated. The results are as follows:

[0030] Numbering Salinity / (mg / L) Oil degradation rate / (%) A 6000 88.6 B 7000 82.9 C 8000 80.8

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PUM

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Abstract

The invention relates to a culture method of petroleum degrading bacteria. The culture method is characterized by comprising the following steps: (1) preparing a petroleum degrading bacterium culture medium; (2) activating the petroleum degrading bacteria in the petroleum degrading bacterium culture medium; (3) taking 200 microliters of a diluted petroleum degrading bacterium solution and the like by utilizing a pipette respectively. The culture method of the petroleum degrading bacteria has the advantages that the petroleum degrading bacteria cultured by the culture method have the advantages of good low-temperature resisting performance and good salt-resisting performance, is applicable to an actual condition of oilfield wastewater treatment and does not pollute the environment.

Description

technical field [0001] The invention relates to a method for cultivating petroleum-degrading bacteria. Background technique [0002] It is estimated that about 1×109 tons of petroleum and its products enter groundwater, surface water and soil through various channels every year in the world. With the rapid economic growth, the task of environmental governance of petroleum in my country is becoming increasingly urgent. Adding efficient petroleum-degrading bacteria It is imperative to strengthen the degradation of petroleum pollutants to achieve the purpose of controlling petroleum pollution. In general, chemical and physical treatment methods are costly and cause secondary pollution, while microbial treatment is relatively low-cost and environmentally friendly. In the field of oilfield sewage treatment, microbial technology is widely used, but the biggest problem is the lack of efficient petroleum-degrading functional bacteria with crude oil as the substrate. Contents of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
CPCC12N1/00
Inventor 张峰
Owner XIAN HUANUO ENVIRONMENTAL PROTECTION CO LTD