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The use of the extract of Pachyphylla alkaloids for the preparation of drugs for inhibiting emt-6 breast cancer cells

A technology of breast cancer cells and EMT-6, which is applied in the field of preparing drugs for inhibiting EMT-6 breast cancer cells, to achieve low production costs, definite anti-tumor effects, and obvious tumor effects

Active Publication Date: 2021-03-19
HEBEI RUILONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: to provide the extract of the alkaloids of the barley building with anti-tumor effect, which solves the technical problem that there is no preparation and utilization of the alkaloids of the barley building in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of the alkaloid extract of the double building, comprises the following steps:

[0030] Step 1: Take 1000g of the medicinal material of Chonglou, add 5000ml of 4mol / l hydrochloric acid solution, soak at room temperature for 48 hours, heat and extract at 60°C for 5 times, each time for 2 hours, filter, and collect the filtrate for later use;

[0031] Step 2: adjust the pH value of the filtrate to 10-14, refrigerate at 4°C, let stand for 24 hours, centrifuge, collect the precipitate, wash with water until the eluate is neutral;

[0032] Step 3: Dissolve the washed to neutral precipitate with ethanol, add 40g of macroporous adsorption resin to absorb for 48 hours, filter, collect the macroporous adsorption resin and pack it into a column, then use 1000ml of water, 1000ml of 30% ethanol aqueous solution, 1000ml of 50% ethanol in sequence Ethanol aqueous solution, 1000ml 70% ethanol aqueous solution elution, the elution speed is counted as 0.2ml / min pe...

Embodiment 2

[0035] The preparation method of the alkaloid extract of the double building, comprises the following steps:

[0036] Step 1: Take 1000g of the medicinal material of Chonglou, add 5000ml of 3mol / l sulfuric acid solution, soak at room temperature for 72 hours, heat and extract at 50°C for 3 times, each time for 4 hours, filter, and collect the filtrate for later use;

[0037] Step 2: adjust the pH value of the filtrate to 14, refrigerate at 4°C, let stand for 48 hours, centrifuge, collect the precipitate, wash with water until the eluate is neutral;

[0038] Step 3: Dissolve the washed to neutral precipitate with ethanol, add 20g of macroporous adsorption resin to absorb for 24 hours, filter, collect the macroporous adsorption resin and pack it into a column, then use 2000ml of water, 2000ml of 30% ethanol aqueous solution, 2000ml of 50% ethanol in sequence Ethanol aqueous solution, 2000ml 70% ethanol aqueous solution elution, the elution rate is calculated as 0.4ml / min per gra...

Embodiment 3

[0041] Antineoplastic effects of alkaloid extracts from Pachyphyllum pachyrhiza.

[0042] Test sample: take the extract obtained in Example 1 as a sample.

[0043] Control sample: doxorubicin.

[0044] Dosage selection and test substance administration method: According to the recommended human dosage of the sample, the recommended human oral dosage is 50mg per person (adult) 3 times a day, and the adult body weight is calculated as 60kg, and the equivalent dose is 0.83mg / kgBW, Set up 2.1mg / kgBW (low-dose group), 4.2mg / kgBW (middle-dose group), 8.3mg / kgBW (high-dose group) (respectively equivalent to 2.5, 5, 10 times the recommended dosage of human body) dose test groups, At the same time, a model control group and a blank control group were set up.

[0045] Animals: SPF grade BABLc mice, female, weighing 18.0-20.0 g.

[0046] Method: EMT-6 cells in the logarithmic growth phase were digested with 0.25% trypsin to detach the wall, then the digestion was terminated, RPMI-1640...

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Abstract

The invention discloses a paris polyphylla alkaloid extract with an anti-tumor effect and a preparation method and application thereof, and solves the technical problem that in the prior art, a preparation and utilization method of a paris polyphylla alkaloid component is not provided. The paris polyphylla alkaloid extract is prepared by extracting paris polyphylla with an acidic solution, then alkalizing, precipitating, dissolving precipitate, applying the dissolved precipitate to macroporous resin, and eluting with alcohol. The preparation method of the paris polyphylla alkaloid extract comprises the following steps: adding the paris polyphylla into the acidic aqueous solution, soaking, heating, extracting, filtering, adjusting the pH value of a filtrate till an alkaline state, centrifuging and collecting the precipitate; adding the precipitate into ethanol, dissolving the precipitate, and applying the dissolved precipitate to the macroporous adsorption resin for adsorption, then sequentially eluting the macroporous adsorption resin column with water, a 30% aqueous ethanol solution, a 50% ethanol solution and a 70% aqueous ethanol solution, and collecting an eluate obtained by using 70% ethanol; and concentrating, and drying at low temperature to obtain the paris polyphylla alkaloid extract. The invention further discloses application of the paris polyphylla alkaloid extract to preparation of anti-tumor medicines. The preparation method is simple and convenient in process and easy and convenient to operate; and the obtained paris polyphylla alkaloid extract has an exact anti-tumor effect.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the use of the extract of the alkaloids of Pachyphylla barbata for preparing the medicine for inhibiting EMT-6 breast cancer cells. Background technique [0002] Chonglou, formerly known as Zhaxiu, was first recorded in "Shen Nong's Materia Medica", and was listed as a lower grade. Chonglou is the dried rhizome of Paris polyphylla Smith var.yunnanensis (Franch.) Hand.-Mazz. or Paris polyphylla Smith var.chinensis (Franch.) Hara of Liliaceae plant. Excavated in autumn, removed fibrous roots, washed and dried in the sun. Mainly produced in Yunnan, Sichuan, Guangxi, Shaanxi, Jiangxi, Jiangsu and other provinces. Chonglou bitter in taste, slightly cold in nature; slightly poisonous. Return liver channel. There is heat-clearing and toxic substances removing, reducing swelling and relieving pain, cooling the liver and relieving convulsion, and is used for boils and ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/439A61P35/00
CPCA61K36/896A61K2236/33A61K2236/55
Inventor 孔利文辜晓平
Owner HEBEI RUILONG BIOTECHNOLOGY CO LTD
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