Detection kit applicable for controlling gait of amble of Yanqi horse and application

Abstract

The invention discloses a detection kit applicable for controlling a gait of amble of Yanqi horse and application. Specific primers are designed according to a gene sequence of a Yanqi horse DMRT3, the positive primer is 5'-AGCGACAAAGACACCGACCAG-3', and the negative primer is 5'-TTGCCAAAGTATTCCTCAGCATCA-3'; by taking a sample genome DNA as a template, PCR (Polymerase Chain Reaction) amplification is carried out, and electrophoresis analysis is carried out after enzyme digestion on a product subjected to PCR amplification is carried out, and if double stripes appear, and Yanqi horse are determined as the Yanqi horse having the gait of amble; if single stripe or three stripes appear, Yanqi horse are determined as the Yanqi horse having the gait of non-amble, and Yanqi horse having the gait of amble can be judged and picked by utilizing the difference of electrophoretic stripes. According to the detection kit disclosed by the invention, a detection method of the Yanqi horse having the gait of amble is short in detection period and high in detection efficiency; moreover, the sensitivity of the detection kit can reach 90 percent, and the specificity can reach 100 percent.

Description

technical field [0001] The invention relates to a method for identifying horse breeds, in particular, the invention relates to the technical field of a kit for detecting contralateral gait Yanqi horses. Background technique [0002] Yanqi horse is an ancient fine horse breed, currently mainly distributed in Hejing County, Heshuo County, Yanqi County, Bohu County and other areas in Bayingoleng Prefecture, Xinjiang; according to records, it existed as early as 2000 years ago The horse breed of this breed is based on cross breeding with Mongolian horses and crossed with tall, cold-resistant and good-walking horse breeds from the Volga River Basin brought by the Donggui tribe. Under the special natural conditions of the Bayinbulak alpine grassland, This breed was formed by the careful grazing management and domestication of Mongolian herdsmen; Yanqi horses are relatively thick and heavy, with an average height of 142 cm for males and 135 cm for females; Day and night trips incl...

AI Technical Summary

Problems solved by technology

[0005] In the prior art, the contralateral gait of the horse is caused by a gene mutation that can be inherited, and it is disclosed that the DMRT3 gene of the horse affects the movement mode of the horse. Although the primer sequence is disclosed, the primer sequence is used When detecting the DMRT3 gene of Yanqi horses, the specificity and efficiency of the amplification are low, which easily leads to false triggers, and the detection period is long, the detection efficiency is low, and the sensitivity and specificity are low, which urgently needs to be solved; the present invention aims to provide The detection kit and application applicable to the contralateral gait control of Yanqi horses, the operation method is simple and fast, and the detection sensitivity and specificity for Yanqi horses are high, which effectively solves the problem of specificity and efficiency of amplification in the prior art. Low, easy to cause false triggers, long detection cycle, low detection efficiency, low sensitivity and specificity

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