Use of Eucalyptus grandis egrzfp6 in improving plant adaptation to stress under osmotic stress

A technology of osmotic stress and Eucalyptus grandis, applied in the field of plant biology, can solve the problems of intolerance to drought, loss of eucalyptus production, etc.

Active Publication Date: 2020-10-20
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is sensitive to low temperature and is not resistant to drought and salinity, which seriously limits the expansion of its cultivation range; adverse weather conditions such as low temperature and drought often cause losses to eucalyptus production

Method used

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  • Use of Eucalyptus grandis egrzfp6 in improving plant adaptation to stress under osmotic stress
  • Use of Eucalyptus grandis egrzfp6 in improving plant adaptation to stress under osmotic stress
  • Use of Eucalyptus grandis egrzfp6 in improving plant adaptation to stress under osmotic stress

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Experimental program
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Embodiment 1

[0025] 1. Materials and methods

[0026] 1.1 Materials

[0027] Eucalyptus grandis seedlings grow in the nursery of Zhejiang Agriculture and Forestry University. The selected test materials were 6-month-old seedlings with consistent growth. Arabidopsis wild type is COL. All experimental treatments were carried out in a snijders microclimate controlled growth chamber (MC1000, Netherlands). The culture conditions are: Arabidopsis: 24°C for 15h during the day, 22°C for 9h at night; Eucalyptus macrophylla: 25°C for 15h during the day, and 22°C for 12h at night. The light intensity is 150μmol·m-2s-1, and the relative humidity (RH) is 70%.

[0028] 1.2 EgrZFP6 protein sequence analysis

[0029] Download the full-length sequence of EgrZFP6 from phytozome (http: / / www.phytozome.net / search.php), design primers, and verify the full-length sequence, and post its encoded protein sequence at https: / / blast.ncbi .nlm.nih.gov / Blast.cgi conducts Blast comparison, selects 5 protein sequenc...

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Abstract

The invention discloses applications of a eucalyptus grandis C2H2 zinc-finger structural protein transcription factor EgrZFP6 in improving the stress adaptation under the osmotic stress of plants. According to the method disclosed by the invention, a 35S:: EgrZFP6 overexpression vector is constructed, and genetic transformation of arabidopsis thaliana is carried out by adopting an inflorescence infection method; compared with the control, after the EgrZFP6 overexpression arabidopsis thaliana transformation plant is treated by PEG (1g.L<-1> or above), the increase and extension of the lateral roots can be greatly promoted, and thus the root architecture of the plants is changed.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and specifically relates to the use of Eucalyptus grandis EgrZFP6 in improving plants' adaptability to stress under osmotic stress. Background technique [0002] Abiotic stresses such as low temperature and drought can restrict plant growth and development, and have a serious impact on agricultural and forestry production. Studying the physiological and molecular mechanisms of plant stress resistance is of great significance for improving plant abiotic stress resistance (Dos Reis et al., 2012; Roychoudhury et al., 2015). In order to cope with adversity stress, plants have formed a certain response mechanism in the long-term evolution process. After the plant senses the adversity signal, it will change a series of metabolic processes through corresponding gene regulation to produce a response to the stress, such as increasing the content of soluble sugar and increasing the resistance to stress. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H5/06A01H6/20
CPCC12N15/8261C12N15/8273
Inventor 程龙军童再康王晓荣徐凤
Owner ZHEJIANG FORESTRY UNIVERSITY
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