Method for detecting non-lactoprotein in milk based on gel electrophoresis and chemometrics

A chemometrics, milk protein technology, applied in the field of non-dairy protein detection in cow milk based on gel electrophoresis and chemometrics, can solve the problem of inability to achieve chromatography, mass spectrometry and spectral experimental conditions, limited use value, lack of detection resources, etc. problem, to achieve the effect of simple and fast operation, stable results and low cost

Inactive Publication Date: 2017-09-15
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

The purpose of adulterating other non-milk proteins is to increase the protein content of milk, but other structures and different types of proteins will be introduced in the process. Therefore, although chromatography, mass spectrometry and spectroscopic techniques can be used to achieve the purpose of detection, however,

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  • Method for detecting non-lactoprotein in milk based on gel electrophoresis and chemometrics
  • Method for detecting non-lactoprotein in milk based on gel electrophoresis and chemometrics
  • Method for detecting non-lactoprotein in milk based on gel electrophoresis and chemometrics

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Embodiment Construction

[0021] The present invention will be further elaborated below in conjunction with accompanying drawings and examples.

[0022] The specific implementation steps are as follows:

[0023] 1. Sample Collection and Preparation

[0024] 16 kinds of fresh raw milk were collected from Qingdao, Shandong. There are 7 kinds of non-dairy protein, including 2 kinds of soybean protein, 2 kinds of pea protein and 3 kinds of whey protein. The liquid milk sample was freeze-dried, and a kind of adulterated milk was selected as an adulterated substrate to prepare a 5 mg / mL milk mother liquid. Centrifuge at 10000rpm for 20min. The sample is divided into three layers, the upper layer is milk fat, the middle layer is soluble protein, and the middle layer is taken for further analysis. According to 4 adulteration ratios: 1%, 3%, 5%, 10% (w / w) to simulate adulteration. The experiments were repeated in triplicate.

[0025] 2. Configuration of SDS-PAGE gel electrophoresis and configuration of st...

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Abstract

The invention belongs to the technical field of food safety detection and particularly relates to a method for detecting non-lactoprotein in milk based on gel electrophoresis and chemometrics. The method includes: using real milk and to-be-detected milk samples as the research objects, and using the SDS-PAGE protein detection technology to collect the gel map of the samples; then using the chemometrics to obtain the global visual main component score plot of the real milk and the to-be-detected milk samples so as to detect the non-lactoprotein in non-targeted milk. The method is low in cost, simple and fast to operate, stable in result and high in accuracy and has potential application and popularization prospect in milk quality detection.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a method for detecting non-dairy protein in milk based on gel electrophoresis and chemometrics. Background technique [0002] Food safety is closely related to human life and is a topic of widespread concern. Among them, food adulteration is one of the important aspects leading to food safety problems. Cow's milk is widely favored by consumers because of its rich nutritional value. However, driven by economic interests, adding fake protein with low value and low cost to milk has become one of the main ways of milk adulteration. The purpose of adulterating other non-milk proteins is to increase the protein content of milk, but other structures and different types of proteins will be introduced in the process. Therefore, although chromatography, mass spectrometry and spectroscopic techniques can be used to achieve the purpose of detection, however, due t...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/44747
Inventor 陆维盈杜丽娟张亚琼牛宇戈
Owner SHANGHAI JIAO TONG UNIV
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