Nano-cone structure composite for capturing cancer cells, and preparation method and application thereof
A composite material and nano-cone technology, which is applied in the direction of tumor/cancer cells, biochemical equipment and methods, animal cells, etc., can solve the problems of complex process and easy lodging of nano-cone structure, and achieve simple method, stable nano-cone structure, low cost effect
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Embodiment 1
[0039] (1) The specification of sheet-shaped conductive substrate titanium is 10×10×1mm 3 , super-clean the substrate with deionized water, 99.7% absolute ethanol and 99.5% acetone respectively for 20 minutes;
[0040] (2) Select the three-electrode mode, the conductive substrate is the working electrode, the copper sheet is the counter electrode, the saturated calomel electrode is the reference electrode, the concentration of pyrrole in the electrolyte solution is 0.2mol / L, and the concentration of hydrochloric acid is 0.25mol / L , the electrochemical reaction is controlled by chronoamperometry, the reaction potential (relative to the reference electrode) is 0.8V, and the reaction time is 20 seconds. A layer of dense and uniform black polypyrrole is deposited on the titanium electrode, which is soaked in deionized water to Remove the unreacted pyrrole and hydrochloric acid on the surface to obtain a titanium electrode deposited with polypyrrole;
[0041] (3) Select the three-...
Embodiment 2
[0045] (1) Sheet conductive substrate (conductive glass) specification is 10×10×1mm 3 , super-clean the substrate with deionized water, 99.7% absolute ethanol and 99.5% acetone respectively for 20 minutes;
[0046] (2) Select the three-electrode mode, the conductive substrate is the working electrode, the copper sheet is the counter electrode, the saturated calomel electrode is the reference electrode, the concentration of pyrrole in the electrolyte solution is 0.2mol / L, and the concentration of hydrochloric acid is 0.25mol / L , the electrochemical reaction is controlled by chronoamperometry, the reaction potential (relative to the reference electrode) is 0.8V, the reaction time is 20 seconds, a layer of dense and uniform black polypyrrole is deposited on the conductive glass electrode, and it is soaked in deionized water To remove unreacted pyrrole and hydrochloric acid on the surface, obtain a conductive glass electrode deposited with polypyrrole;
[0047] (3) Select the thr...
Embodiment 3
[0049] (1) The specification of sheet-shaped conductive substrate titanium is 10×10×1mm 3 , super-clean the substrate with deionized water, 99.7% absolute ethanol and 99.5% acetone respectively for 20 minutes;
[0050] (2) Select the three-electrode mode, the conductive substrate is the working electrode, the copper sheet is the counter electrode, the saturated calomel electrode is the reference electrode, the concentration of pyrrole in the electrolyte solution is 0.2mol / L, and the concentration of hydrochloric acid is 0.25mol / L , the electrochemical reaction is controlled by chronoamperometry, the reaction potential (relative to the reference electrode) is 0.8V, and the reaction time is 20 seconds. A layer of dense and uniform black polypyrrole is deposited on the titanium electrode, which is soaked in deionized water to Remove the unreacted pyrrole and hydrochloric acid on the surface to obtain a titanium electrode deposited with polypyrrole;
[0051] (3) Select the three-...
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