Nano-cone structure composite for capturing cancer cells, and preparation method and application thereof

A composite material and nano-cone technology, which is applied in the direction of tumor/cancer cells, biochemical equipment and methods, animal cells, etc., can solve the problems of complex process and easy lodging of nano-cone structure, and achieve simple method, stable nano-cone structure, low cost effect

Active Publication Date: 2017-09-19
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researchers have used the AAO template method to prepare materials for the capture and release of cancer cells. However, the process of template removal involved in this method is achieved by alkali etching, which affects the activity of biomolecules on the surface of the material. At the same time, the process It is more complex, and the prepared nanocone structure is easy to lodging

Method used

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  • Nano-cone structure composite for capturing cancer cells, and preparation method and application thereof
  • Nano-cone structure composite for capturing cancer cells, and preparation method and application thereof
  • Nano-cone structure composite for capturing cancer cells, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) The specification of sheet-shaped conductive substrate titanium is 10×10×1mm 3 , super-clean the substrate with deionized water, 99.7% absolute ethanol and 99.5% acetone respectively for 20 minutes;

[0040] (2) Select the three-electrode mode, the conductive substrate is the working electrode, the copper sheet is the counter electrode, the saturated calomel electrode is the reference electrode, the concentration of pyrrole in the electrolyte solution is 0.2mol / L, and the concentration of hydrochloric acid is 0.25mol / L , the electrochemical reaction is controlled by chronoamperometry, the reaction potential (relative to the reference electrode) is 0.8V, and the reaction time is 20 seconds. A layer of dense and uniform black polypyrrole is deposited on the titanium electrode, which is soaked in deionized water to Remove the unreacted pyrrole and hydrochloric acid on the surface to obtain a titanium electrode deposited with polypyrrole;

[0041] (3) Select the three-...

Embodiment 2

[0045] (1) Sheet conductive substrate (conductive glass) specification is 10×10×1mm 3 , super-clean the substrate with deionized water, 99.7% absolute ethanol and 99.5% acetone respectively for 20 minutes;

[0046] (2) Select the three-electrode mode, the conductive substrate is the working electrode, the copper sheet is the counter electrode, the saturated calomel electrode is the reference electrode, the concentration of pyrrole in the electrolyte solution is 0.2mol / L, and the concentration of hydrochloric acid is 0.25mol / L , the electrochemical reaction is controlled by chronoamperometry, the reaction potential (relative to the reference electrode) is 0.8V, the reaction time is 20 seconds, a layer of dense and uniform black polypyrrole is deposited on the conductive glass electrode, and it is soaked in deionized water To remove unreacted pyrrole and hydrochloric acid on the surface, obtain a conductive glass electrode deposited with polypyrrole;

[0047] (3) Select the thr...

Embodiment 3

[0049] (1) The specification of sheet-shaped conductive substrate titanium is 10×10×1mm 3 , super-clean the substrate with deionized water, 99.7% absolute ethanol and 99.5% acetone respectively for 20 minutes;

[0050] (2) Select the three-electrode mode, the conductive substrate is the working electrode, the copper sheet is the counter electrode, the saturated calomel electrode is the reference electrode, the concentration of pyrrole in the electrolyte solution is 0.2mol / L, and the concentration of hydrochloric acid is 0.25mol / L , the electrochemical reaction is controlled by chronoamperometry, the reaction potential (relative to the reference electrode) is 0.8V, and the reaction time is 20 seconds. A layer of dense and uniform black polypyrrole is deposited on the titanium electrode, which is soaked in deionized water to Remove the unreacted pyrrole and hydrochloric acid on the surface to obtain a titanium electrode deposited with polypyrrole;

[0051] (3) Select the three-...

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Abstract

The invention belongs to the technical field of medical biomaterials, and discloses a nano-cone structure composite for capturing cancer cells, and a preparation method and application thereof. The method includes: electrodepositing chlorine-doped polypyrrole on the surface of a conductive substrate by the aid of chronoamperometry; selecting a three-electrode pattern to deposit polypyrrole / biotin material with nano-cone structure on a working electrode by the aid of chronopotentiometry by using a conductive metal as a counter electrode, the polypyrrole-deposited conductive substrate as the working electrode and a buffer solution containing pyrrole and biotin as an electrolyte; activating the working electrode deposited the polypyrrole / biotin material with nano-cone structure, culturing in streptavidin solution, performing grafting reaction with antibodies, and culturing in BSA (bull serum albumin) solution to obtain the nano-cone structure composite. The method is simple and low in cost, the nano-cone structure in the composite is stable, and cancer cells can be effectively captured and nondestructively released.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and relates to a nano-cone structure composite material and a preparation method thereof. The nano-cone structure composite material is used for rapidly capturing cancer cells and releasing the cells without damage. Background technique [0002] Isolation of cancer cells is of great significance in basic biology, development of clinical diagnosis, and research on therapeutic modalities. At present, a technology that relies on the specific binding of antibody antigens to separate and purify cancer cells by recognizing markers on the surface of target cell membranes has been developed. Compared with traditional benchtop methods, current platform-based technologies have the advantages of enhanced cell recovery and improved purity and capture of target cells. Although previous studies have focused on enhancing the capture rate and sensitivity, the lossless release of cells and the rapid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12M47/04G01N33/54346
Inventor 宁成云王珍高于鹏谭帼馨胡诗迁姚甜甜罗雯
Owner SOUTH CHINA UNIV OF TECH
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