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Application of oxidized resveratrol or oxidized resveratrol combined with antibiotics in the preparation of antifungal infection products

A technology for oxidizing resveratrol and antibiotics, applied in the field of medicine, can solve the problems of safety doubts, unbearable, high recurrence rate, and achieve the effects of good safety, reduced usage, and natural ingredients

Active Publication Date: 2020-08-11
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Oral drugs are mainly antifungal drugs, such as Sporanox (alias: itraconazole, itraconazole, itraconazole, itraconazole), but oral drugs often cause greater harm to the human body; Over time, it is easy to cause many side effects such as drug resistance of fingernails to Trichophyton rubrum, and the safety is constantly being questioned
Medicines for external use, such as miconazole nitrate, compound benzoic acid ointment and other western medicines, are applied to the affected area to kill fungi. However, these medicines are often very irritating and may cause burning and irritation when used. Pain, unbearable in severe cases, and high recurrence rate

Method used

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  • Application of oxidized resveratrol or oxidized resveratrol combined with antibiotics in the preparation of antifungal infection products
  • Application of oxidized resveratrol or oxidized resveratrol combined with antibiotics in the preparation of antifungal infection products
  • Application of oxidized resveratrol or oxidized resveratrol combined with antibiotics in the preparation of antifungal infection products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of oxidized resveratrol

[0033] (1) Get the mulberry branches free from diseases and insect pests, dry and pulverize them and cross a 100-mesh sieve to obtain the mulberry branches powder;

[0034] (2) The mulberry branch powder prepared in step (1) is added in absolute ethanol, and microwave extraction is carried out three times under 350W, each time 30min, collects each extract and mixes, and extract is concentrated to extractum, i.e. To obtain the Mori Branch extract, the mass volume ratio of the Mori Branch powder to absolute ethanol is 1mg:20mL; the Mori Branch Extract is dissolved in water to obtain an aqueous solution of the Mori Branch Extract, and the Mori Branch Extract aqueous solution is After extraction with petroleum ether, add it to ethyl acetate, extract again to obtain the extract, concentrate the extract to extract, and obtain the ethyl acetate extract of Mori twigs, the aqueous solution of Mori twig extracts and petroleum ether The volum...

Embodiment 2

[0037] 1. Detection of minimum inhibitory concentration (MIC) of oxidized resveratrol on Trichophyton rubrum

[0038]The oxidized resveratrol sample solution prepared in Example 1 was added to the RPM-1640 medium by the double dilution method until the concentration of oxidized resveratrol was 2.000, 1.000, 0.500, 0.250, 0.125, 0.063, 0.031, 0.015mg / mL. Take a 96-well bacterial culture plate and add 100 μL to each well with a concentration of 10 6 CFU / mL of Trichophyton rubrum bacteria solution and 100 μL of oxidized resveratrol sample solution of various concentrations, and set blank control (medium + bacteria solution without oxidized resveratrol sample solution) and solvent control (DMSO solution + bacterial solution), and a control culture plate (without bacterial solution) was set at the same time. After culturing in a constant temperature incubator at 28°C for 93 hours, the minimum inhibitory concentration of oxidized resveratrol on Trichophyton rubrum was 0.5 mg / mL th...

Embodiment 3

[0063] 1. Detection of minimum inhibitory concentration (MIC) of oxidized resveratrol against Candida albicans

[0064] The oxidized resveratrol sample solution prepared in Example 1 was added to the RPM-1640 medium by the double dilution method until the concentration of oxidized resveratrol was 2.000, 1.000, 0.500, 0.250, 0.125, 0.063, 0.031, 0.015mg / mL. Take a 96-well bacterial culture plate and add 100 μL to each well with a concentration of 10 6 CFU / mL of Candida albicans bacteria solution and 100 μL of oxidized resveratrol solutions of various concentrations, and set up blank control (medium without oxidized resveratrol sample solution+bacteria solution) and solvent control (DMSO solution+bacteria solution) solution), and a control culture plate (without bacterial solution) was set at the same time. Cultivate in a constant temperature incubator at 28°C for 48 hours, and observe with the naked eye that the minimum inhibitory concentration of oxidized resveratrol to Cand...

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Abstract

The invention relates to application of oxidized resveratrol and combined oxidized resveratrol and antibiotic in the preparation of an anti-fungal-infection product, belonging to the technical field of medicines. Oxidized resveratrol contains nautical components, is good in safety and has good antibacterial capacity to trichophyton rubrum and Candida albicans. When the oxidized resveratrol and an antibiotic, namely econazole nitrate jointly act on the two bacteria, the antibacterial effects on the two bacteria are superior to the antibacterial effects of dependent independent oxidized resveratrol and antibiotic on the two bacteria under the same use concentration, so that natural oxidized resveratrol is used for replacing partial econazole nitrate so as to inhibit fungi, the use amount of econazole nitrate can be reduced, and the research and development of novel, efficient and natural anti-fungal-infection drugs are benefited.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of oxidized resveratrol or oxidized resveratrol combined with econazole nitrate in the preparation of antifungal infection products. Background technique [0002] With the gradual improvement of living standards, people's awareness of health and disease prevention is also constantly strengthened. Fungi are a type of bacteria that can cause various diseases in animals, plants and humans. Different fungi can cause disease in different ways, which can be divided into the following categories: (1) pathogenic fungal infections: caused by exogenous fungi, such as dermatophytes; (2) conditional pathogenic fungal infections: caused by endogenous fungi (3) fungal hypersensitivity diseases: inhalation or ingestion of mycelium or spores can cause urticaria, asthma, etc.; (4) fungal poisoning: eating molds containing mycotoxins (5) Mycotoxins: related to tumori...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/05A61K31/4174A61P31/10
CPCA61K31/05A61K31/4174A61K2300/00
Inventor 徐立逯海朋贾亚楠喻艳
Owner SOUTHWEST UNIV
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