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A biosorbent for adsorbing thallium ions and its preparation method and application

A biosorbent and ion technology, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as unsatisfactory treatment effects, high cost, and secondary pollution

Active Publication Date: 2020-07-14
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these physical and chemical methods can remove a certain amount of heavy metals to a certain extent, they generally have problems such as high cost, unsatisfactory treatment effect, and even secondary pollution.

Method used

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  • A biosorbent for adsorbing thallium ions and its preparation method and application
  • A biosorbent for adsorbing thallium ions and its preparation method and application
  • A biosorbent for adsorbing thallium ions and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: Isolation and identification of bacterial strain FP-JCCW

[0041] (1) Isolation and purification of strain FP-JCCW

[0042] The strain FP-JCCW was isolated and screened from plants in the Dabaoshan mining area in Shaoguan, Guangdong. The culture medium for the isolated strain FP-JCCW was selected from Chapei's medium.

[0043] The composition of the Cha's culture medium is: NaNO 3 3g,K 2 HPO 4 ·3H 2 O 1g, MgSO 4 ·7H 2O 0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.01g, sucrose 30g, agar 20g, distilled water 1000mL. The preparation process of this Chaspor medium can refer to the prior art, and will not be described in detail here.

[0044] Make a spore suspension from the strain FP-JCCW freshly cultured to the logarithmic phase, apply 1mL of the spore suspension to a medium containing a certain concentration of thallium, and place it in a constant temperature incubator at 28°C for 48 hours to observe whether there are colonies If there is colony growth, draw...

Embodiment 2

[0051] Example 2: Preparation of biosorbent and its adsorption performance on thallium

[0052] (1) Preparation of biosorbent

[0053] The cultured strain FP-JCCW was collected by filtration with 8 layers of gauze, the collected mycelium was washed 3 times with deionized water, dried naturally, placed in an oven at 60°C to a constant weight, and after cooling, the mycelium was ground into Fine powder, sieved, set aside.

[0054] (2) Adsorption test method

[0055] Take 50ml of different concentrations of Tl solution in a 100ml Erlenmeyer flask, with 0.1mol / L HNO 3 or NaOH to Tl + The pH value of the solution is adjusted to a series of values ​​(3~9), Tl + The initial concentration variable is 20-100mg / L, the contact time variable is 10-120min, the shaker speed variable is 30-180r / min, the temperature variable is 20-40℃, and the strain biomass variable is 0.5-5g / L, each variable set a set of experiments, and each set of experiments set 3 repetitions to test the adsorption...

Embodiment 3

[0068] Embodiment 3: Characterization analysis of bacterial strain FP-JCCW

[0069] (1) FT-IR Characterization Analysis

[0070] Take an appropriate amount of dry strain powder and grind it with KBr, tablet it, and then use it on a TENSOR 27 Fourier transform infrared spectrometer (Bruker, Germany) at 400 to 4000 cm. -1 scan in range.

[0071] Figure 8 It is the FT-IR figure of bacterial strain FP-JCCW before and after adsorption of T1. from Figure 8 It can be seen that the strain FP-JCCW adsorbed Tl at 3390cm -1 There is an absorption peak at 2925cm, which is caused by the expansion and contraction of the O-H bond; -1 and 2856cm -1 There is an absorption peak at each place, which is caused by the expansion and contraction of the C-H bond; at 1743cm -1 There is an absorption peak at , which may be caused by the stretching of aldehyde-CHO; at 1635cm -1 The absorption peak at is produced by the stretching vibration of the C=O bond and the bending vibration of the N-H b...

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Abstract

The invention belongs to the technical field of sewage treatment, and discloses a biosorbent, which at least contains thallium-resistant fungal strain FP-JCCW or a treatment product of the strain FP-JCCW, and the strain FP-JCCW was published on May 09, 2017 It is preserved in the China Center for Type Culture Collection with the preservation number CCTCC M 2017249. The preparation method of the treated product of the bacterial strain FP-JCCW is: wash the mycelium of the collected bacterial strain FP-JCCW with deionized water, after natural drying, place in an oven to dry to constant weight, cool the The mycelium is ground into fine powder, sieved, and set aside. The invention also discloses the application of the biosorbent in adsorbing thallium in water bodies. The biosorbent prepared by the strain FP‑JCCW has a strong adsorption capacity for thallium in water, and can be used for the remediation and treatment of thallium pollution in water. The biosorbent described in the invention can be used as an environmental functional material to be developed.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and relates to biological treatment of sewage, in particular to a thallium-resistant fungal strain, a biosorbent prepared by the strain, and its application in thallium-polluted bioremediation projects. Background technique [0002] In recent years, the pollution of heavy metals has become increasingly serious. As a toxic and harmful heavy metal, thallium (Tl) has increasingly obvious impact on the ecological environment. Thallium is unevenly distributed in natural soil, but due to human industrial activities and mining operations, thallium-containing wastewater enters the natural world during the enrichment process of thallium, and thallium has caused great harm to human life, production and the natural environment. . [0003] Thallium pollution of water bodies has had an impact on the ecological environment and the health of the people. Thallium-containing wastewater flows into the n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/645C02F101/20
CPCC02F3/347C02F2101/20C12N1/145C12R2001/645
Inventor 龙建友陈永亨肖唐付李伙生周舒薏
Owner GUANGZHOU UNIVERSITY
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