A biosorbent for adsorbing thallium ions and its preparation method and application
A biosorbent and ion technology, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as unsatisfactory treatment effects, high cost, and secondary pollution
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Embodiment 1
[0040] Embodiment 1: Isolation and identification of bacterial strain FP-JCCW
[0041] (1) Isolation and purification of strain FP-JCCW
[0042] The strain FP-JCCW was isolated and screened from plants in the Dabaoshan mining area in Shaoguan, Guangdong. The culture medium for the isolated strain FP-JCCW was selected from Chapei's medium.
[0043] The composition of the Cha's culture medium is: NaNO 3 3g,K 2 HPO 4 ·3H 2 O 1g, MgSO 4 ·7H 2O 0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.01g, sucrose 30g, agar 20g, distilled water 1000mL. The preparation process of this Chaspor medium can refer to the prior art, and will not be described in detail here.
[0044] Make a spore suspension from the strain FP-JCCW freshly cultured to the logarithmic phase, apply 1mL of the spore suspension to a medium containing a certain concentration of thallium, and place it in a constant temperature incubator at 28°C for 48 hours to observe whether there are colonies If there is colony growth, draw...
Embodiment 2
[0051] Example 2: Preparation of biosorbent and its adsorption performance on thallium
[0052] (1) Preparation of biosorbent
[0053] The cultured strain FP-JCCW was collected by filtration with 8 layers of gauze, the collected mycelium was washed 3 times with deionized water, dried naturally, placed in an oven at 60°C to a constant weight, and after cooling, the mycelium was ground into Fine powder, sieved, set aside.
[0054] (2) Adsorption test method
[0055] Take 50ml of different concentrations of Tl solution in a 100ml Erlenmeyer flask, with 0.1mol / L HNO 3 or NaOH to Tl + The pH value of the solution is adjusted to a series of values (3~9), Tl + The initial concentration variable is 20-100mg / L, the contact time variable is 10-120min, the shaker speed variable is 30-180r / min, the temperature variable is 20-40℃, and the strain biomass variable is 0.5-5g / L, each variable set a set of experiments, and each set of experiments set 3 repetitions to test the adsorption...
Embodiment 3
[0068] Embodiment 3: Characterization analysis of bacterial strain FP-JCCW
[0069] (1) FT-IR Characterization Analysis
[0070] Take an appropriate amount of dry strain powder and grind it with KBr, tablet it, and then use it on a TENSOR 27 Fourier transform infrared spectrometer (Bruker, Germany) at 400 to 4000 cm. -1 scan in range.
[0071] Figure 8 It is the FT-IR figure of bacterial strain FP-JCCW before and after adsorption of T1. from Figure 8 It can be seen that the strain FP-JCCW adsorbed Tl at 3390cm -1 There is an absorption peak at 2925cm, which is caused by the expansion and contraction of the O-H bond; -1 and 2856cm -1 There is an absorption peak at each place, which is caused by the expansion and contraction of the C-H bond; at 1743cm -1 There is an absorption peak at , which may be caused by the stretching of aldehyde-CHO; at 1635cm -1 The absorption peak at is produced by the stretching vibration of the C=O bond and the bending vibration of the N-H b...
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