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Biological absorbent capable of adsorbing thallium ions, preparation method and applications thereof

A biosorbent and ion technology, which is applied in the application fields of biosorbent, biological treatment of sewage, and thallium pollution bioremediation engineering, can solve the problems of unsatisfactory treatment effect, high cost, secondary pollution and so on.

Active Publication Date: 2017-10-03
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these physical and chemical methods can remove a certain amount of heavy metals to a certain extent, they generally have problems such as high cost, unsatisfactory treatment effect, and even secondary pollution.

Method used

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  • Biological absorbent capable of adsorbing thallium ions, preparation method and applications thereof
  • Biological absorbent capable of adsorbing thallium ions, preparation method and applications thereof
  • Biological absorbent capable of adsorbing thallium ions, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Isolation and identification of strain FP-JCCW

[0041] (1) Separation and purification of strain FP-JCCW

[0042] The strain FP-JCCW was isolated and screened from the plants in the Dabaoshan mining area in Shaoguan, Guangdong. The culture medium of the isolated strain FP-JCCW is Chashi medium.

[0043] The composition of the Cha's medium is: NaNO 3 3g, K 2 HPO 4 ·3H 2 O 1g, MgSO 4 ·7H 2 O 0.5g, KCl0.5g, FeSO 4 ·7H 2 O 0.01g, sucrose 30g, agar 20g, distilled water 1000mL. The preparation process of this Chashi medium can refer to the prior art, and will not be described in detail here.

[0044] The freshly cultivated strain FP-JCCW in the logarithmic phase was made into a spore suspension, and 1 mL of the spore suspension was applied to a certain concentration of thallium-containing medium, and placed in a 28°C constant temperature incubator for 48 hours to observe whether there are colonies If a colony grows, another 1 mL of the spore suspension is inoculated in...

Embodiment 2

[0051] Example 2: Preparation of biosorbent and its adsorption performance for thallium

[0052] (1) Preparation of biosorbent

[0053] Filter the cultured strain FP-JCCW with 8 layers of gauze, wash the collected mycelium with deionized water 3 times, dry it naturally, and place it in an oven at 60°C to a constant weight. After cooling, grind the mycelium into Fine powder, sieved and set aside.

[0054] (2) Adsorption test method

[0055] Take 50ml Tl solution of different concentration in a 100ml Erlenmeyer flask, use 0.1mol / L HNO 3 Or NaOH will Tl + Adjust the pH value of the solution to a series of values ​​(3~9), Tl + The initial concentration variables are 20-100mg / L, the contact time variables are 10-120min, the shaker speed variables are 30-180r / min, the temperature variables are 20-40℃, and the strain biomass variables are 0.5-5g. / L, set up a set of experiments for each variable, and set up 3 repetitions for each set of experiments to test the adsorption rate and adsorption...

Embodiment 3

[0068] Example 3: Characterization and analysis of strain FP-JCCW

[0069] (1) FT-IR characterization analysis

[0070] Take an appropriate amount of dried bacterial strain powder and grind together with KBr, press the tablet, and then use the TENSOR 27 Fourier Transform Infrared Spectrometer (Bruker, Germany) at 400~4000cm -1 Scan within range.

[0071] Figure 8 FT-IR diagrams before and after Tl adsorption of strain FP-JCCW. From Figure 8 It can be seen that the strain FP-JCCW is at 3390cm before adsorbing Tl -1 There is one absorption peak at 2925cm, which is caused by O-H bond stretching and contraction; -1 And 2856cm -1 There is one absorption peak at each place, which is caused by the expansion and contraction of the C-H bond; at 1743cm -1 There is an absorption peak at 1635cm, which may be caused by aldehyde group-CHO stretching; -1 The absorption peak at is caused by the stretching vibration of the C=O bond and the bending vibration of the N-H bond in the protein amide; a...

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Abstract

The present invention belongs to the technical field of sewage treatment, and discloses a biological absorbent, which at least comprises a thallium-resistant fungus strain FP-JCCW or the treatment product of the strain FP-JCCW, wherein the strain FP-JCCW is preserved in the China Center for Type Culture Collection on May 09, 2017, and has the preservation number of CCTCC M 2017249. The preparation method comprises: washing the mycelium of the collected strain FP-JCCW by using deionized water, carrying out natural air drying, placing in a dry oven, drying to achieve a constant weight, cooling, grinding the mycelium into fine powder, and screening so as to be spare. The invention further discloses applications of the biological sorbent in the adsorption of thallium in a water body. According to the present invention, the biological absorbent prepared from the strain FP-JCCW can strongly adsorb the thallium in the water body, can be used for the remediation treatment of the thallium-polluted water body, and can be used as the environmental functional material to be developed.

Description

Technical field [0001] The invention belongs to the technical field of sewage treatment, and relates to biological treatment of sewage, in particular to a thallium-resistant fungal strain, a biosorbent prepared from the strain, and its application in a thallium pollution bioremediation project. Background technique [0002] In recent years, the pollution of heavy metals has become more and more serious. As a toxic and harmful heavy metal, thallium (Tl) has an increasingly obvious impact on the ecological environment. Thallium is unevenly distributed in the natural soil, but due to human industrial activities and mining operations, the thallium-containing wastewater enters the nature during the process of thallium enrichment, and thallium has caused great harm to human life, production and the natural environment. . [0003] Thallium pollution in water bodies has already affected the ecological environment and the health of the people. Wastewater containing thallium flows into the...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34C12R1/645C02F101/20
CPCC02F3/347C02F2101/20C12N1/145C12R2001/645
Inventor 龙建友陈永亨肖唐付李伙生周舒薏
Owner GUANGZHOU UNIVERSITY
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