Application of Rapeseed bnaspt3 Gene in Promoting Dicotyledonous Silique Growth

Abstract

The invention discloses an application of a brassica napus BnaSPT3 gene in promoting growth of pods of dicotyledons. A base sequence of the brassica napus BnaSPT3 gene is shown as SEQ ID NO.1. The application comprises the following steps: (1) linking the brassica napus BnaSPT3 gene into a plant expression vector, so that a recombinant expression vector is constructed; and (2) transforming the recombinant expression vector into a recipient plant. The invention also discloses the recombinant expression vector containing the brassica napus BnaSPT3 gene and a transformant containing the recombinant expression vector. According to the application provided by the invention, by cloning the brassica napus BnaSPT3 gene, implementing genetic transformation in arabidopsis thaliana and through excessive expression of the exogenous brassica napus BnaSPT3 gene, the size of arabidopsis thaliana spt mutant pods can be obviously increased, and the number of seeds can be recovered. On the basis of genetic engineering technology, over-expression of the brassica napus BnaSPT3 gene in a receptor is achieved, and the size of the pods is changed; therefore, the invention is conducive to high-yield researches.

Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to the application of rapeseed BnaSPT3 gene in promoting the growth of dicotyledons siliques. Background technique

[0002] Rapeseed is one of the main oil crops in my country. The planting and production of rapeseed directly affect the safety of oilseed food in my country. And in the world, the position of rapeseed in the oil crops is also the top priority. At present, the output of rapeseed in our country is still far from meeting the domestic demand, and increasing the output of rapeseed is an urgent task. Silique is an important part of rapeseed yield components, and silique and its related characters have direct or indirect effects on rapeseed yield. Rapeseed silique, as the fruiting organ of rapeseed, is not only an important storage organ, but also an important photosynthetic organ, with dual functions of "sink" and "source". The growth and development of siliques s...

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