Molecular marker applied to detecting sugarcane ratoon stunting diseases and application thereof

A molecular marker, dwarf disease technology, applied in the field of molecular biology, can solve the problems of difficult to achieve large-scale application, unstable detection results, pathogen mutation, etc., and achieve stable detection results, good specificity and good repeatability. Effect

Inactive Publication Date: 2017-10-03
SUZHOU COSMETIC MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to limited conditions and mutations in pathogens, the detection results are unstable, and it is difficult to achieve large-scale application

Method used

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  • Molecular marker applied to detecting sugarcane ratoon stunting diseases and application thereof
  • Molecular marker applied to detecting sugarcane ratoon stunting diseases and application thereof
  • Molecular marker applied to detecting sugarcane ratoon stunting diseases and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A molecular marker for detecting dwarf disease of sugarcane perennial roots, the molecular marker and its sequence are: P1, SEQ ID NO.1-2; P2, SEQ ID NO.3-4; P3, SEQ ID NO.5-5 6.

[0029] The 5' ends of the upstream primers of the molecular markers P1, P2 and P3 were modified by amination.

[0030] The application of the molecular marker in the preparation of a kit for detecting sugarcane perennial dwarf disease.

[0031] The components of the kit include: molecular markers, dNTP, LA Taq, PCR reaction buffer, MgCl 2 , double distilled water.

[0032] The steps of the kit detecting sugarcane perennial dwarf disease include:

[0033] (1) Sampling: extract the genomic DNA of the sugarcane leaf sample;

[0034] (2) Using the DNA extracted in step (1) as a PCR template, and using P1 as a specific primer, carry out the first round of PCR amplification;

[0035] (3) Get the amplified product of step (2), dilute it 100 times and use it as the template for the second round ...

Embodiment 2

[0044] A molecular marker for detecting dwarf disease of sugarcane perennial roots, the molecular marker and its sequence are: P1, SEQ ID NO.1-2; P2, SEQ ID NO.3-4; P3, SEQ ID NO.5-5 6.

[0045] The 5' ends of the upstream primers of the molecular markers P1, P2 and P3 were modified by amination.

[0046] The application of the molecular marker in the preparation of a kit for detecting sugarcane perennial dwarf disease.

[0047] The components of the kit include: molecular markers, dNTP, LA Taq, PCR reaction buffer, MgCl 2 , double distilled water.

[0048] The steps of the kit detecting sugarcane perennial dwarf disease include:

[0049] (1) Sampling: extract the genomic DNA of the sugarcane leaf sample;

[0050] (2) Using the DNA extracted in step (1) as a PCR template, and using P1 as a specific primer, carry out the first round of PCR amplification;

[0051] (3) Get the amplified product of step (2), dilute it 200 times and use it as the template for the second round ...

Embodiment 3

[0060] A molecular marker for detecting dwarf disease of sugarcane perennial root, the molecular marker and its sequence are: P1, SEQ ID NO.1-2; P2, SEQ ID NO.3-4; P3, SEQ ID NO.5-5 6.

[0061] The 5' ends of the upstream primers of the molecular markers P1, P2 and P3 were modified by amination.

[0062] The application of the molecular marker in the preparation of a kit for detecting sugarcane perennial dwarf disease.

[0063] The components of the kit include: molecular markers, dNTP, LA Taq, PCR reaction buffer, MgCl 2 , double distilled water.

[0064] The steps of the kit detecting sugarcane perennial dwarf disease include:

[0065] (1) Sampling: extract the genomic DNA of the sugarcane leaf sample;

[0066] (2) Using the DNA extracted in step (1) as a PCR template, and using P1 as a specific primer, carry out the first round of PCR amplification;

[0067] (3) Get the amplified product of step (2), dilute it 500 times and use it as the template for the second round o...

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Abstract

The invention discloses a molecular marker applied to detecting sugarcane ratoon stunting diseases and application thereof. The molecular marker and a sequence of the molecular marker are P1, SEQ ID NO.1 to 2; P2, SEQ ID NO.3 to 4; P3, SEQ ID NO.5 to 6. (1) The molecular marker disclosed by the invention has the advantages of simpleness, quickness and accuracy; (2) the molecular marker disclosed by the invention is more suitable for mass detection of the field sugarcane ratoon stunting diseases and has good repeatability, stable detection results and no false negative phenomenon; (3) the molecular marker disclosed by the invention has good specificity and high detection flexibility.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a method for detecting pathogenic bacteria by means of molecular biology, in particular to a molecular marker for detecting sugarcane perennial dwarf disease and its application. Background technique [0002] Sugarcane ratroot dwarf disease is an important bacterial disease that commonly occurs in various sugarcane growing areas in the world. The disease is caused by a xylem-restricted pathogen. Cane plants have no typical external symptoms after sugarcane infection, and only in very serious cases do they show external symptoms such as short plants and thin cane stems, and these symptoms are easily confused with poor growth caused by factors such as drought and insufficient nutrients. It is difficult to judge whether the sugarcane plant is diseased or not by the naked eye alone, which leads to the unintentional spread of sugarcane perennial root dwarf disease through the diseased ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689
Inventor 金仲恩陈晋纳欧阳智琨喻国贞张帆全春兰
Owner SUZHOU COSMETIC MATERIALS
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