Strawberry subculture multiplication medium and application thereof

A technology of subculture proliferation and culture medium, applied in the field of strawberry subculture proliferation medium, can solve the problems of poor transformation mechanism, slow promotion and application of virus-free vaccines, etc. Degradation, the effect of increasing the enthusiasm for production

Pending Publication Date: 2017-10-20
黔东南民族职业技术学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the serious disconnection in the fields of scientific research, production and distribution, and economic costs, the transformation mechanism is not smooth, and the promotion and application of virus-free vaccines is progressing slowly.

Method used

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  • Strawberry subculture multiplication medium and application thereof
  • Strawberry subculture multiplication medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The invention provides a method for rapid propagation of strawberry virus-free tissue culture, comprising the following steps:

[0036] S101: selecting stolons of strawberry plants as explants, and performing heat treatment and disinfection treatment in sequence.

[0037] S102: peeling off shoot tips from the sterilized explants, and then inducing adventitious buds.

[0038] S103: Transplant the induced adventitious buds into a subculture medium for multi-generation subculture to obtain test-tube plantlets. Among them, each liter of subculture proliferation medium includes the following raw material components: 0.5 mg of 6-benzylaminopurine, 0.01 mg of naphthaleneacetic acid, 30 g of white sugar, 3.8 g of agar, and the balance is MS medium, and the pH value of the adjustment medium is 6.2. The multi-generational subculture culture is 3 generations; the specific culture conditions include: the relative air humidity is 95%, and the light time is 11h·d -1, the light int...

Embodiment 2

[0042] The invention provides a method for rapid propagation of strawberry virus-free tissue culture, comprising the following steps:

[0043] S101: selecting stolons of strawberry plants as explants, and performing heat treatment and disinfection treatment in sequence.

[0044] S102: peeling off shoot tips from the sterilized explants, and then inducing adventitious buds.

[0045] S103: Transplant the induced adventitious buds into a subculture medium for multi-generation subculture to obtain test-tube plantlets. Among them, each liter of subculture proliferation medium includes the following raw material components: 1.0mg of 6-benzylaminopurine, 0.05mg of naphthaleneacetic acid, 15g of white sugar, 5.0g of agar, and the balance is MS medium, and the pH value of the adjustment medium is 6.0. The multi-generational subculture culture is 5 generations; the specific culture conditions include: the relative air humidity is 85%, and the light time is 13h·d -1 , the light intens...

Embodiment 3

[0049] The invention provides a method for rapid propagation of strawberry virus-free tissue culture, comprising the following steps:

[0050] S101: selecting stolons of strawberry plants as explants, and performing heat treatment and disinfection treatment in sequence.

[0051] S102: peeling off shoot tips from the sterilized explants, and then inducing adventitious buds.

[0052] S103: Transplant the induced adventitious buds into a subculture medium for multi-generation subculture to obtain test-tube plantlets. Among them, each liter of subculture proliferation medium includes the following raw material components: 0.1 mg of 6-benzylaminopurine, 0.05 mg of naphthaleneacetic acid, 30 g of white sugar, 5.0 g of agar, and the balance is MS medium, and the pH value of the adjustment medium is 6.1. The multi-generation subculture culture is divided into 4 generations; the culture conditions specifically include: the relative air humidity is 80%, and the light time is 12h·d -1...

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Abstract

The invention relates to the technical field of plant cultivation, in particular to a strawberry subculture proliferation medium and application thereof. The subculture growth medium includes the following raw material components per liter: 0.5 mg of 6-benzylaminopurine, 0.01 mg of naphthaleneacetic acid, 30 g of sugar, 3.8 g of agar, and the balance is MS medium. Then it is used in the strawberry virus-free tissue culture and rapid propagation method to carry out multi-generation subculture and multiplication culture. By adopting the subculture proliferation medium provided by the invention, the value-added rate of strawberry test-tube plantlets can be increased by about 8 times, the average plant height can be increased by about 25.85%, the average number of leaves can be increased by about 16.03%, and the average number of reproductive stolons per plant can be increased by about 109.51%. , The final strawberry yield increased by about 53.98%, and the diseased number and survival rate of strawberries were significantly improved; in addition, the subculture propagation medium of the present invention is convenient to apply, low in cost, easy to popularize, and significantly improves the production of strawberry growers. positivity.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a strawberry subculture proliferation medium and application thereof. Background technique [0002] As we all know, plant cell totipotency means that each cell of a plant contains all the genetic information of the species, thus having the genetic ability to develop into a complete plant; the concept of cell totipotency has become the theoretical basis for plant tissue culture. According to this theory, the isolated plant shoot tip is cultivated in an artificially controlled environment through the regulation of plant hormones to dedifferentiate and redifferentiate to form a complete plant. [0003] Strawberry is also called raspberry, ground berry, etc., and belongs to Rosaceae, Fragaria, perennial herb. Strawberry fruit is delicious, red and tender, with juicy pulp, special rich fruit aroma, rich in vitamin C, vitamin A, carotene, etc., and has high nutritional and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 郑晓峰黄刚何宪江付燕万新屏周黎
Owner 黔东南民族职业技术学院
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