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New application of traditional Chinese medicine preparation

A technology of traditional Chinese medicine preparation and rhubarb, which is applied in drug combinations, medical formulas, surgical drugs, etc., can solve the problems of strong limitations of anti-adhesion measures, complicated preparation, and easy bleeding of preparations, and achieve the goal of inhibiting the proliferation of peritoneal fibroblasts Effect

Inactive Publication Date: 2017-10-20
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anti-adhesion measures with a single effect have strong limitations. For example, anti-inflammatory agents have inconsistent drug effects in animal models and clinical experiments; preparations with strong fibrinolytic properties can easily cause bleeding and affect wound healing. Some drugs also need gel as a carrier to exert their effects. Anti-adhesion effect; material preparations have completely different therapeutic effects before and after trauma, are expensive, and are difficult to be widely used in surgical clinics; although some material preparations have been mass-produced, the preparation is complicated, requiring hemostasis, poor flexibility, and complicated fixation techniques, etc. Many shortcomings are difficult to meet the clinical needs of endoscopic surgery

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of embodiment 1 Chinese medicine preparation

[0021] It is made of rhubarb, peach kernels, Corydalis, radish seeds, rhubarb: peach kernels: Corydalis: radish = 1:1:1:1, add 70% ethanol 15 times the total amount of decoction pieces (the amount of 95% ethanol = 150×70 %÷95%) were refluxed and extracted twice, each time for 2h, filtered, combined extracts, ethanol was recovered from the filtrate under reduced pressure, and concentrated until no alcohol smell. Combine the above medicinal residues with safflower (safflower: rhubarb = 3:5), add 20 times the total amount of decoction pieces and decoct three times, 1 hour each time, filter, combine the decoction, and concentrate the filtrate to a relative density of 1.09~ 1.11 (60°C), cool, add ethanol to 50% alcohol content, stir well, let it stand for 48 hours, recover ethanol from the filtrate, take the supernatant and add Glauber's salt (Grassie: Rhubarb = 1:1) to shrink to a relative density of 1.12...

Embodiment 2

[0022] Example 2 Water decoction prevents and treats abdominal cavity adhesion evaluation

[0023] animal

[0024] 96 healthy male SD rats, weighing 220-250 g, were provided by Nanjing Qinglongshan Animal Breeding Farm (SPF grade), certificate number SCXK (Su) 2017-0001. The disposal of animals during the experiment complied with the regulations of the Ministry of Science and Technology's "Guiding Opinions on Treating Experimental Animals Kindly". Rats were acclimated to the environment in the animal room for 7 days before the experiment. The room temperature was 18-25°C and the relative humidity was 65%-70%.

[0025] instrument

[0026] Realtime PCR instrument (ABI stepone plus, USA), vertical electrophoresis equipment electrophoresis instrument (Bio-Rad, USA), chemiluminescent imager (Bio-Rad ChemiDoc XRS+, USA). Nucleic acid and protein analyzer (US Lifetechnologies Qubit 2.0).

[0027] method

[0028] Seventy-two rats were randomly divided into 3 groups, sham operatio...

Embodiment 3

[0046] Example 3 Effect Test on Fibroblast Type III Collagen mRNA Expression

[0047] cell

[0048] Mouse embryonic fibroblast NIH / 3T3 cells were purchased from the Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

[0049] Main reagents and their preparation

[0050] Trizol, reverse transcription kit and real-time fluorescent quantitative PCR kit were purchased from Dalian Bao Biological Engineering Co., Ltd. PCR tips and centrifuge tubes are disposable RNase-free products; other reagents are analytically pure, purchased from Nanjing Chemical Reagent Company;

[0051] Primary solution preparation

[0052] Preparation of PBS buffer: Accurately weigh 8.0g of NaCl, 0.2g of KCl, NaCl 2 HPO 4 12H 2 O 3.48g, KH 2 PO 4 0.2g, dissolved in 1000mL ultrapure water in turn, mixed evenly, dispensed, autoclaved and stored in a 4°C refrigerator for later use.

[0053] High-glucose DMEM medium: follow the instructions. Dissolve each bag...

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Abstract

The invention discloses an application of a traditional Chinese medicine preparation which is made from rhubarb root and rhizome, peach seeds, crystallized sodium sulfate, safflowers, yanhusuo and radish seeds to prepare a medicine used for inhibiting abdominal fibroblast propagation, and discloses an application of the traditional Chinese medicine preparation to prepare a medicine for lowering fibroblast III collagen mRNA expression level. Based on the prior art, the traditional Chinese medicine preparation made from rhubarb root and rhizome, peach seeds, crystallized sodium sulfate, safflowers, yanhusuo and radish seeds is subjected to deep research. It is found from the research that the traditional Chinese medicine preparation can lower fibroblast III collagen mRNA expression level, inhibits abdominal fibroblast propagation, and provides a new idea and prospects for a medicine used for preventing and treating abdominal adhesion.

Description

technical field [0001] The invention belongs to traditional Chinese medicine technology, and in particular relates to a new application of traditional Chinese medicine preparations. Background technique [0002] Postoperative peritoneal adhesions refer to the fibrous band between the abdominal viscera or the abdominal wall formed during the repair of peritoneal injury after pelvic and abdominal surgery. %, the incidence rate after lower abdominal surgery is 67-93%. Once the disease is formed, it can cause a series of complications such as abdominal pain, abdominal distension, and intestinal obstruction. Even the second operation will prolong the operation time, increase the probability of damage to the intestines and other organs, and bring double burdens to patients mentally and economically. [0003] The common clinical manifestations are chronic abdominal pain, abdominal distension, constipation, or even repeated attacks of intestinal obstruction after surgery. Some pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/736A61P41/00A61K33/04
CPCA61K33/04A61K36/286A61K36/31A61K36/66A61K36/708A61K36/736A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2300/00
Inventor 曾莉李文林李正钧杨丽丽卞尧尧颜帅朱燕
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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