Preparation method of antioxidant effective components of radix rubiae and application

An effective ingredient and antioxidant technology, applied in the field of preparation of madder extract, can solve the problems of organic residue, complex process, low yield of antioxidant effective components, etc., and achieves low cost, simple steps, and strong scavenging of DPPH free radicals. the effect of the ability

Inactive Publication Date: 2017-10-20
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problems of complex process in the existing method, the existence of organic residues, and the low yield of anti-oxidative active components, and provides a preparation method and application of anti-oxidative active components of madder

Method used

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  • Preparation method of antioxidant effective components of radix rubiae and application
  • Preparation method of antioxidant effective components of radix rubiae and application
  • Preparation method of antioxidant effective components of radix rubiae and application

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specific Embodiment approach 1

[0023] Specific embodiment one: the preparation method of the anti-oxidative effective components of Rubia in this embodiment comprises the following steps:

[0024] 1. Take madder medicinal material powder, add ethanol with a mass percentage concentration of 94% to 96%, the mass ratio of madder medicinal material powder to ethanol is 1: (9 to 11), soak for 12 to 14 hours, then ultrasonically treat for 30 to 40 minutes, Filtrate to collect solid phase A and liquid phase A respectively;

[0025] Add solid phase substance A into ethanol with a mass percentage concentration of 94% to 96% and soak for 8 to 10 hours, the mass ratio of solid phase substance A to ethanol is 1: (9 to 11), then sonicate for 30 to 40 minutes, and filter , collect the solid-phase substance B and the liquid-phase substance B respectively;

[0026] Add solid phase substance B into ethanol with a mass percentage concentration of 94% to 96% and soak for 4 to 6 hours. The mass ratio of solid phase substance ...

specific Embodiment approach 2

[0030] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the mobile phase of the preparative liquid chromatography in step 3 is methanol-0.2% phosphoric acid aqueous solution. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0031] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the gradient elution program in step three is 0min → 10min → 20min → 30min → 45min → 50min, the volume ratio of methanol is 30% → 60% → 70% → 75% → 100% → 100%. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a preparation method and application of anti-oxidative active ingredients of madder, and relates to a preparation method and application of madder extract. The purpose of the method is to solve the problems of complex process, organic residues and low yield of anti-oxidation effective components in the existing method. Method: 1. Take madder medicinal material powder and add ethanol to soak, sonicate, and collect solid-phase substance A and liquid-phase substance A; add solid-phase substance A to ethanol and soak, sonicate, collect solid-phase substance B and liquid-phase substance B; Substance B is soaked in ethanol, ultrasonicated, and solid phase substance C and liquid phase substance C are collected; liquid phase substance A, liquid phase substance B and liquid phase substance C are mixed to obtain an extract, and concentrated to obtain an extract; 2. Extract the extract Put on a macroporous resin chromatography column, collect the eluate, concentrate under reduced pressure to obtain solid D; 3. dissolve D with ethanol, perform gradient elution, collect the eluate, and obtain an effective component after drying. The method is simple and rapid, has low cost and high yield, and does not involve organic solvents. The present invention is due to the extraction of the anti-oxidative active ingredients of madder.

Description

technical field [0001] The invention relates to a preparation method and application of madder extract. Background technique [0002] Rubia is the dried root and rhizome of Rubia cordifolia L., a Rubiaceae plant, and is also one of the traditional Chinese medicines commonly used in my country. It mainly contains anthraquinone and its glycosides, naphthoquinone and its glycosides, polysaccharides, cyclohexyl peptides, trace elements and other components. "Chinese Pharmacopoeia" records that it is cold in nature, bitter in taste, and belongs to the Liver Meridian. It has a significant curative effect on cardiovascular and cerebrovascular diseases. Modern studies have shown that madder has anti-oxidation, white blood cell increase and immunosuppressive effects, liver protection, antipyretic and analgesic effects, cough and expectorant effects, and has also achieved good results in antibacterial, anti-inflammatory, and tumor inhibition. In the process of using oxygen in the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P39/06A61K8/9789A61Q19/08A23L33/105
CPCA61K36/74A23L33/105A23V2002/00A61K8/97A61K2236/333A61K2236/39A61K2236/55A61Q19/08
Inventor 刘丽娟
Owner HEILONGJIANG UNIV
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