A kind of enrichment and solid-phase derivatization pretreatment method of phosphate sugars

A technology for phosphate sugars and sugars, which is applied in the field of phosphate sugar enrichment and solid-phase derivatization pretreatment to improve the detection sensitivity of mass spectrometry, lower detection limits, and remove matrix interference.
CN107290459BActive Publication Date: 2019-12-06DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2019-12-06

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Abstract

The invention belongs to the technical field of inorganic materials and analysis, in particular to a metal ion affinity chromatography material (SiO 2 @PD‑Ti 4+ ) pretreatment method for enrichment and solid-phase derivatization of phosphate carbohydrates and its application. First, phosphate sugars were enriched on SiO by metal chelation 2 @PD‑Ti 4+ On the microspheres, the enriched phosphate sugars are then subjected to solid-phase derivatization. The method can not only improve the retention and resolution of sugar phosphate substances on reversed-phase liquid chromatography, but also improve the detection sensitivity of mass spectrometry for sugar phosphate substances with low abundance. The pretreatment method of the present invention has the advantages of simple pretreatment process, rapidity, good repeatability, low detection limit and the like.
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Description

technical field

[0001] The invention belongs to the technical field of inorganic materials and analysis, and is a kind of material based on metal ion affinity chromatography (SiO 2 @PD-Ti 4+ ) pretreatment method for enrichment and solid-phase derivatization of phosphate sugars and its application. Background technique

[0002] Phosphoric sugars such as glucose-6-phosphate, ribose-5-phosphate, erythrose-4-phosphate and glyceraldehyde-3-phosphate play important roles in many life processes. They are important intermediate metabolites in central carbon metabolism, such as glycolysis, gluconeogenesis, pentose phosphate pathway and tricarboxylic acid cycle metabolic pathway, and can participate in the regulation of tumor energy metabolism. At present, liquid chromatography-mass spectrometry (LC-MS) technology has been widely used in the detection of phosphate sugars due to its advantages of high sensitivity and high resolution. However, due to the strong polarity of phosphate...

Claims

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