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A planting method and technology of Taxus chinensis, which are applied in the directions of fertilization method, application, organic fertilizer and the like, can solve the problems of large fluctuation of Taxol content of Taxus, difficult to standardize planting in a large area, etc., and achieve the effect of increasing Taxol content
Inactive Publication Date: 2017-10-27
GUILIN MINGXING BIOTECH
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From the above results, it is not difficult to find that the paclitaxel content of taxu
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[0030] The yew planting method includes the following steps:
[0031] 1) Cultivation environment: Choose Guilin, Guangxi at an altitude of 600-1000m, with gentle terrain, slope less than 35°, loose soil, deep, convenient drainage, moist, organic matter and acidic soil or mountainous land;
[0032] 2) Planting and afforestation: From June to December, the forest land will be cleaned and prepared in a strip-like manner, deep plowing and plowing, forming a vegetable border, the size is 40cmx40cmx30cm, the distance between the rows is 0.4mx1.5m, and the density is 16,500 Plant / hm 2 ; Simultaneously apply sufficient base fertilizer, mix evenly with the depth, plant yew seedlings, artificially cultivated 2.5-year-old Yunnan yew cutting seedlings, ground diameter above 0.3cm, seedling height above 50cm, or artificial The cultivated 1.5-year-old seedlings of Taxus chinensis var. mairei, the average seedling height is 25cm, the average ground diameter is 0.08cm, the seedlings should not be ...
experiment example
[0043] Paclitaxel detection: Clean the fresh branches and leaves of Taxus chinensis, dry them, pulverize with a grinder, and pulverize with a 10mm mesh grinder, with a particle size of 2-10mm, and detect the paclitaxel content. Take an appropriate amount of sample, accurately weigh it, dissolve it in a 10ml or 25ml volumetric flask with methanol (chromatographically pure), dissolve it completely under ultrasound for 3-5min, and dilute to the mark. Take a certain amount of the dissolved sample and centrifuge it in a high-speed centrifuge at 10,000 rpm for 5 min. Take an appropriate amount of the supernatant after centrifugation, filter it through a 0.45μm filter membrane with a syringe, and pour it into a sample bottle for testing. Use high performance liquid chromatography to measure the sample to be tested. Detection conditions: injection volume is 5μl, C18 column (250X4.6mm), column temperature 25℃, acetonitrile-water (34:69) isocratic elution for 10min, and then increase th...
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Abstract
The invention discloses a planting method for taxus chinensis. The planting method for taxus chinensis comprises the following steps: (1) selection of a cultivation environment; (2) planting and afforestation: from June to December, cleaning and carrying out soil preparation on a forest land in a zonal mode, carrying out deep tillage and intensive farming to obtain a garden, meanwhile applying sufficient base fertilizers, mixing uniformly along with deep tillage, planting taxus chinensis seedlings which cannot be inclined, enabling root systems to be expanded horizontally and forwards, covering the root systems with soil under the condition that roots are prevented from being pressed, and then covering hole surfaces with several loosened soil to enable the hole surfaces to be slightly higher than the ground and prevent water accumulation in the rainy season; (3) nurse and management: supplementing new seedlings in time if some of seedlings are dead one week after the seedlings are planted, applying a nutrient solution once every week to thoroughly irrigate roots in the first year after the seedlings survive, applying fertilizers on time, loosening the soil and removing weeds in time, and draining water to prevent the circumstance that young trees are soaked by accumulated water so that the young trees are damaged or dead due to heat; and (4) preventing and controlling harmful organisms in the forest. The special nutrient solution is applied to the taxus chinensis, and therefore, the taxol content in the taxus chinensis can be increased.
Description
Technical field [0001] The invention relates to the technical field of forest tree cultivation, in particular to a method for planting yew. Background technique [0002] Taxus L. is an evergreen tree or shrub of the Taxus family. There are 11 species in the world, distributed in the temperate and subtropical regions of the northern hemisphere. In 1996, Alpine Forest et al. collected 7 taxus species with higher taxol content at home and abroad, such as brevifolia (T. brevifolia, Vancouver, Canada) and Media (T.media, Victoria University campus, Canada) , European yew (T.baccata, North Korea), Northeast yew (T.cuspida, Northeast China), Korean yew (T.kereoensis, North Korea), Yunnan yew (T.wallichiana, Yunnan Province, China), Tibet yew Taxus wallichi-ann (Taxus wallichi-ann, Tibet, China), found that Taxus brevifolia has the highest content of paclitaxel in the bark (Taxus brevifolia was the first species found to contain paclitaxel by the American Cancer Institute), and it has b...
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