Regenerated tussah silk fibroin protein solution and preparation method thereof

Abstract

The invention relates to a regenerated tussah silk fibroin protein solution and a preparation method thereof. The solution consists of pure tussah silk fibroin protein, the silk fibroin protein mainly has a nanofiber structure, the diameter of the silk fibroin protein is 50nm or smaller, and the length is between 100nm and 500nm. According to the preparation method of the solution, natural tussah silk is directly used as a raw material, and organic solvent dissolution and deionized water dialysis treatment are carried out to obtain the solution. According to the preparation method provided by the invention, the organic solvent is used for direct dissolution, the strong degrading problem during high-temperature dissolution of high-concentration neutral salt is avoided, the process is simple, the solution obtained by the method does not have reagent residual, contains a nanometer bionic structure simultaneously, and has excellent biological security.

Description

technical field

[0001] The invention relates to a natural polymer solution and a preparation method thereof, in particular to a regenerated tussah silk fibroin protein solution and a preparation method thereof. Background technique

[0002] Biomedical materials are one of the frontier research hotspots in both life sciences and materials science. At present, many degradable polymer materials have been developed and applied in biomedical fields such as tissue engineering, but most of the original research work on these materials belongs to foreign countries and has applied for a number of patents in my country. Therefore, actively looking for suitable raw materials and developing biological materials with my country's independent intellectual property rights are of great significance for reducing the dependence of my country's tissue engineering scaffolds and other biological materials on foreign countries, cultivating new high-tech industries and realizing the sustainable de...

Examples

Embodiment 1

[0035] The natural tussah silk was boiled with 0.5wt% sodium bicarbonate solution for 60 minutes, washed with deionized water and dried to obtain degummed silk. The degummed silk was placed in a beaker, deionized water was added, and then ultrasonic treatment was performed with an ultrasonic power of 5 kW and an ultrasonic treatment time of 40 minutes. At room temperature, the degummed silk was placed in 86 wt% trifluoroacetic acid aqueous solution containing 3% formic acid (based on the mass of trifluoroacetic acid), the mass fraction of silk was 7%, and the regenerated silk fibroin trifluoroacetic acid solution was obtained after magnetic stirring for 10 min. Then the solution was injected into a dialysis bag, placed in flowing deionized water and dialyzed for 3 days at a dialysis temperature of 10°C to obtain a purified aqueous solution of regenerated tussah silk fibroin nanofibers. The silk fibroin in this solution is in the form of nanofibers with a fiber length of 0.1-15...

Embodiment 2

[0039]The natural tussah silk was boiled with 0.05wt% sodium bicarbonate solution for 60 minutes, then fully washed with deionized water, repeated three times and dried to obtain degummed silk. Put the degummed silk in a beaker, add deionized water, and then perform ultrasonic treatment, the ultrasonic power is 3kW, and the ultrasonic treatment time is 35 minutes. At room temperature, the degummed silk was placed in 85wt% trifluoroacetic acid aqueous solution containing 5% formic acid (based on the mass of trifluoroacetic acid), the mass fraction of silk was 8%, and the regenerated silk fibroin organic solution was obtained after magnetic stirring for 5 minutes. Then the solution was injected into a dialysis bag, placed in flowing deionized water and dialyzed for 5 days at a temperature of 4° C. to obtain a purified aqueous solution of regenerated tussah silk fibroin nanofibers. The silk fibroin in this solution is in the form of nanofibers, such as image 3 As shown, the mol...

Embodiment 3

[0043] Natural tussah silk was boiled with 0.25wt% sodium bicarbonate solution for 60 minutes, then fully washed with deionized water, and then dried three times to obtain degummed silk. Put the degummed silk in a beaker, add deionized water, and then perform ultrasonic treatment, the ultrasonic power is 6kW, and the ultrasonic treatment time is 35 minutes. At room temperature, the degummed silk was placed in 88wt% trifluoroacetic acid aqueous solution containing 4% formic acid (based on the mass of trifluoroacetic acid), the mass fraction of silk was 7%, and the regenerated silk fibroin organic solution was obtained after magnetic stirring for 20 minutes. Then inject the solution into a dialysis bag, place it in flowing deionized water and dialyze for 3 days at a temperature of 4°C to obtain a purified aqueous solution of regenerated tussah silk fibroin nanofibers. Silk fibroin film, the protein structure is an amorphous structure, which is transformed into a β-sheet crystal ...

technical field

[0001] The invention relates to a natural polymer solution and a preparation method thereof, in particular to a regenerated tussah silk fibroin protein solution and a preparation method thereof. Background technique

[0002] Biomedical materials are one of the frontier research hotspots in both life sciences and materials science. At present, many degradable polymer materials have been developed and applied in biomedical fields such as tissue engineering, but most of the original research work on these materials belongs to foreign countries and has applied for a number of patents in my country. Therefore, actively looking for suitable raw materials and developing biological materials with my country's independent intellectual property rights are of great significance for reducing the dependence of my country's tissue engineering scaffolds and other biological materials on foreign countries, cultivating new high-tech industries and realizing the sustainable de...

Examples

Embodiment 1

[0035] The natural tussah silk was boiled with 0.5wt% sodium bicarbonate solution for 60 minutes, washed with deionized water and dried to obtain degummed silk. The degummed silk was placed in a beaker, deionized water was added, and then ultrasonic treatment was performed with an ultrasonic power of 5 kW and an ultrasonic treatment time of 40 minutes. At room temperature, the degummed silk was placed in 86 wt% trifluoroacetic acid aqueous solution containing 3% formic acid (based on the mass of trifluoroacetic acid), the mass fraction of silk was 7%, and the regenerated silk fibroin trifluoroacetic acid solution was obtained after magnetic stirring for 10 min. Then the solution was injected into a dialysis bag, placed in flowing deionized water and dialyzed for 3 days at a dialysis temperature of 10°C to obtain a purified aqueous solution of regenerated tussah silk fibroin nanofibers. The silk fibroin in this solution is in the form of nanofibers with a fiber length of 0.1-15...

Embodiment 2

[0039]The natural tussah silk was boiled with 0.05wt% sodium bicarbonate solution for 60 minutes, then fully washed with deionized water, repeated three times and dried to obtain degummed silk. Put the degummed silk in a beaker, add deionized water, and then perform ultrasonic treatment, the ultrasonic power is 3kW, and the ultrasonic treatment time is 35 minutes. At room temperature, the degummed silk was placed in 85wt% trifluoroacetic acid aqueous solution containing 5% formic acid (based on the mass of trifluoroacetic acid), the mass fraction of silk was 8%, and the regenerated silk fibroin organic solution was obtained after magnetic stirring for 5 minutes. Then the solution was injected into a dialysis bag, placed in flowing deionized water and dialyzed for 5 days at a temperature of 4° C. to obtain a purified aqueous solution of regenerated tussah silk fibroin nanofibers. The silk fibroin in this solution is in the form of nanofibers, such as image 3 As shown, the mol...

Embodiment 3

[0043] Natural tussah silk was boiled with 0.25wt% sodium bicarbonate solution for 60 minutes, then fully washed with deionized water, and then dried three times to obtain degummed silk. Put the degummed silk in a beaker, add deionized water, and then perform ultrasonic treatment, the ultrasonic power is 6kW, and the ultrasonic treatment time is 35 minutes. At room temperature, the degummed silk was placed in 88wt% trifluoroacetic acid aqueous solution containing 4% formic acid (based on the mass of trifluoroacetic acid), the mass fraction of silk was 7%, and the regenerated silk fibroin organic solution was obtained after magnetic stirring for 20 minutes. Then inject the solution into a dialysis bag, place it in flowing deionized water and dialyze for 3 days at a temperature of 4°C to obtain a purified aqueous solution of regenerated tussah silk fibroin nanofibers. Silk fibroin film, the protein structure is an amorphous structure, which is transformed into a β-sheet crystal ...

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