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Production method for high-adenosine-content fruiting body of Cordyceps militaris

A technology of adenosine militaris and production method, which is applied in the field of production of high-content adenosine Cordyceps militaris fruiting bodies, can solve the problems of low extraction efficiency and low extraction rate of adenosine, and achieve huge market potential, high extraction rate, and production short cycle effect

Inactive Publication Date: 2017-11-03
李鹄鸣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, 11 nucleoside compounds have been isolated and identified from Cordyceps sinensis and fermented hyphae, including adenine, adenosine, uracil, uridine, guanine, guanosine, hypoxanthine, inosine, and thymine , thymidine and uridine deoxynucleoside, etc. In the prior art, the extraction efficiency of adenosine from the fruiting body of Cordyceps militaris is low, and the extraction rate is low. Therefore, how to improve the existing processing method in the technical route and processing technology, and improve the adenosine Glycoside content is an urgent and important subject

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A high-content adenosine Cordyceps militaris fruiting body, said Cordyceps militaris fruiting body is cultivated in a culture medium, and components in a 2000g medium include shelled peanuts 80g, raw soybeans 80g, rice 450g, tomatoes 40g, dried Silkworm chrysalis powder 40g, fish meal 40g, fresh beef 40g, potato 40g, glucose 10g, yeast powder 5g, sucrose 10g, agar 20g, vitamin B1 20mg, straw extract 450ml and soil extract 450ml.

[0027] In the present embodiment, the preparation method of rice straw extract comprises the following steps:

[0028] A, get straw 45g, add in pulverizer and pulverize, pulverizer rotating speed is 2000 rpm, pulverization time is 10min, obtains rice straw powder;

[0029] B. Add 600g of water to the rice straw powder obtained in step A, boil the water for 50min to obtain a mixed solution, and then cool to normal temperature;

[0030] C. Add the mixed solution obtained in step B into a centrifuge and centrifuge to obtain the rice straw extrac...

Embodiment 2

[0043] A high-content adenosine Cordyceps militaris fruiting body, said Cordyceps militaris fruiting body is cultivated in a culture medium, and components in a 2000g medium include shelled peanuts 120g, raw soybeans 120g, rice 550g, tomatoes 60g, dried Silkworm chrysalis powder 60g, fish meal 60g, fresh beef 60g, potato 60g, glucose 20g, yeast powder 10g, sucrose 20g, agar 30g, vitamin B1 60mg, straw extract 550ml and soil extract 550ml.

[0044] In the present embodiment, the preparation method of rice straw extract comprises the following steps:

[0045] A, get straw 55g, add in pulverizer and pulverize, pulverizer rotating speed is 3000 rpm, pulverization time is 20min, obtains rice straw powder;

[0046] B. Add 800g of water to the rice straw powder obtained in step A, boil the water for 70min to obtain a mixed solution, and then cool to normal temperature;

[0047] C. Add the mixed solution obtained in step B into a centrifuge and centrifuge to obtain the rice straw ext...

Embodiment 3

[0060] A high-content adenosine Cordyceps militaris fruiting body, said Cordyceps militaris fruiting body is cultivated in a culture medium, and components in a 2000g medium include shelled peanuts 90g, raw soybeans 90g, rice 480g, tomatoes 45g, dried Silkworm chrysalis powder 45g, fish meal 45g, fresh beef 45g, potato 45g, glucose 12g, yeast powder 6g, sucrose 12g, agar 22g, vitamin B1 30mg, straw extract 480ml and soil extract 480ml.

[0061] In the present embodiment, the preparation method of rice straw extract comprises the following steps:

[0062] A, get straw 48g, add in pulverizer and pulverize, pulverizer rotating speed is 2200 rpm, pulverization time is 12min, obtains rice straw powder;

[0063] B. Add 650g of water to the rice straw powder obtained in step A, boil the water for 55min to obtain a mixed solution, and then cool to normal temperature;

[0064] C. Add the mixed solution obtained in step B into a centrifuge and centrifuge to obtain the rice straw extrac...

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Abstract

The invention discloses a production method for a high-adenosine-content fruiting body of Cordyceps militaris. The fruiting body of Cordyceps militaris is cultured in a medium. 2000 g of the medium comprises, by weight, 80 to 120 g of shelled peanuts, 80 to 120 g raw soybeans, 450 to 550 g of rice, 40 to 60 g of tomatoes, 40 to 60 g of dry silkworm chrysalis powder, 40 to 60 g of fish meal, 40 to 60 g of fresh beef, 40 to 60 g of potatoes, 10 to 20 g of glucose, 5 to 10 g of yeast powder, 10 to 20 g of sucrose , 20 to 30 g of agar, 20 to 60 mg of vitamin B1, 450 to 550 ml of straw leachate and 450 to 550 ml of soil leachate. The production method provided by the invention is simple in process; and the prepared fruiting body of Cordyceps militaris is high in extraction rate of adenosine.

Description

technical field [0001] The invention relates to the technical field of fruiting bodies of Cordyceps militaris, in particular to a method for producing fruiting bodies of Cordyceps militaris with high content of adenosine. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, Cordyceps militaris, etc., belongs to the same species as Cordyceps sinensis. It is a model species of the genus Cordyceps and belongs to the Ascomycetes subphylum Cordyceps. A complex of subseats and dead pupae. Years of research have shown that Cordyceps militaris has various pharmacological activities such as immune regulation, anti-tumor, anti-virus, and anti-infection, and is widely used in various diseases such as tumors, low immune function, and HIV infection. [0003] Adenosine is an endogenous purine nucleotide with a wide range of physiological activities. The content of adenosine in Cordyceps militaris is similar to that of Cordyceps sinensis, or even higher, m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
CPCA01G18/00C05D9/00C05G3/00C05F1/00C05F1/002C05F11/00C05F11/08C05F11/10
Inventor 李鹄鸣王菊凤韩磊张帆
Owner 李鹄鸣
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