Chlorella and application thereof in sewage degradation

A technology for degrading sewage and chlorella, applied in the field of environmental engineering, can solve problems such as difficult COD treatment, and achieve the effects of easy cultivation, rapid reproduction, and shortened cycle

Active Publication Date: 2017-11-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In order to solve the problem of difficult treatment of total nitrogen, total phosphorus and COD in sewage, the present invention screened and obtained a strain of

Method used

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  • Chlorella and application thereof in sewage degradation
  • Chlorella and application thereof in sewage degradation
  • Chlorella and application thereof in sewage degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Determination of the total nitrogen degradation effect of chlorella

[0030] Chlorella enriched medium (BG-11 medium): NaNO 3 225 mg, K 2 HPO 4 ·3H 2 O 45.6 mg, MgSO 4 ·7H 2 O 125.7 mg, CaCl 2 26.64mg, ferric citrate 75.95mg, EDTA·2Na 10mg, H 2 O 1L, trace elements 1ml / L (H 3 BO 4 28.6g / L, MnCl 2 4H 2 O 18.1g / L, ZnSO 4 ·7H 2 O 2.22g / L, Na 2 MoO 4 2H 2 O 3.9g / L, CuSO 4 ·5H 2 O 0.79g / L, Co(NO 3 ) 2 ·6H 2 O 0.494g / L).

[0031] Simulated sewage medium: KH 2 PO 4 1g, NaCl 3g, K 2 HPO 4 1g, KNO 3 0.72g, C 6 h 12 o 6 3.75g, H 2 O 1L, trace elements 1ml / L (MnCl 2 4H 2 O 2.5g / L, ZnSO 4 ·7H 2 O 2.2g / L, CuSO 4 ·5H 2 O 0.2g / L, MgSO 4 ·7H 2 O 10g / L, CaCl 2 2H 2 O 7.3g / L, CoCl 2 ·6H 2 O 0.5g / L, FeSO 4 ·7H 2 O 5g / L).

[0032] Take 20 μl of the seed solution, inoculate it in 50 ml of BG-11 medium, culture it with shaking at 30°C and 90 rpm for 4 days, and the inoculum size in this example is 3%, then take 3 ml of the enriched me...

Embodiment 2

[0034] Embodiment 2: Determination of the total nitrogen degradation effect of Chlorella with different inoculum amounts

[0035] Chlorella enriched medium (BG-11 medium): NaNO 3 225 mg, K 2 HPO4 ·3H 2 O 45.6 mg, MgSO 4 ·7H 2 O 125.7 mg, CaCl 2 26.64mg, ferric citrate 75.95mg, EDTA·2Na 10mg, H 2 O 1L, trace elements 1ml / L (H 3 BO 4 28.6g / L, MnCl 2 4H 2 O 18.1g / L, ZnSO 4 ·7H 2 O 2.22g / L, Na 2 MoO 4 2H 2 O 3.9g / L, CuSO 4 ·5H 2 O 0.79g / L, Co(NO 3 ) 2 ·6H 2 O 0.494g / L)

[0036] Simulated sewage medium: KH 2 PO 4 1g, NaCl 3g, K 2 HPO 4 1g, KNO 3 0.72g, C 6 h 12 o 6 3.75g, H 2 O 1L, trace elements 1ml / L (MnCl 2 4H 2 O 2.5g / L, ZnSO 4 ·7H 2 O 2.2g / L, CuSO 4 ·5H 2 O 0.2g / L, MgSO 4 ·7H 2 O 10g / L, CaCl 2 2H 2 O 7.3g / L, CoCl 2 ·6H 2 O 0.5g / L, FeSO 4 ·7H 2 O 5g / L)

[0037] Take 20 μl of seed solution, inoculate in 50ml of BG-11 medium, culture at 30°C, 90rpm shaking for 4 days, centrifuge at 5000rpm for 10min, discard the supernatant, and collect ...

Embodiment 3

[0039] Example 3: Effects of different temperatures, inoculum size, carbon-to-nitrogen ratio, and trace element amounts on the degradation of total nitrogen by chlorella

[0040] Take 20 μl of seed solution, inoculate it in BG-11 medium, culture it with shaking at 90 rpm for 4 days at 30°C, centrifuge at 5000 rpm for 10 min, discard the supernatant, and collect algae. According to shown in Table 1 respectively, prepare corresponding simulated sewage culture medium, 250ml triangular flask contains 100ml simulated sewage medium, under corresponding conditions, 90rpm shaking cultivates 6 days, adopts potassium persulfate oxidation-ultraviolet spectrophotometry to measure its degradation total The effect of nitrogen, its degraded total nitrogen result is as shown in table 2, draws its effect curve figure as Figure 4 shown.

[0041] Table 1 Experimental design

[0042]

[0043] Table 2 visual analysis table

[0044]

[0045]

[0046] The results showed that: from the i...

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Abstract

The invention discloses chlorella and application thereof in sewage degradation, and belongs to the technical field of environmental engineering. The chlorella having a preservation number of CCTCC NO:M 2017231 can be used for removing total nitrogen, total phosphorous and COD in wastewater, and the total nitrogen, total phosphorous and COD in wastewater can be removed in an environment-friendly, ecological, efficient and low-price manner. The chlorella is suitable for treating sewage having high water treatment capacity, total nitrogen of about 120mg/L, total phosphorous of about 18mg/L and low COD concentration of about 4200mg/L.

Description

technical field [0001] The invention relates to a strain of chlorella and its application in degrading sewage, belonging to the technical field of environmental engineering. Background technique [0002] In recent decades, people have gradually paid attention to environmental protection, and the state has stepped up efforts to prevent and control polluted water bodies. However, the pollution of rivers and lakes has not been effectively curbed for many years. The pollution load of water bodies has continued to increase, and the problem of eutrophication is still serious. Security remains at risk. [0003] Analyze from the following two aspects: [0004] First, industrial enterprises and sewage treatment plants still have problems such as tail water or reclaimed water nitrogen discharge not up to standard, imperfect discharge standards and insufficient supervision. It is urgent to solve the problems of developing nitrogen removal technology system, improving discharge standar...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F3/32C12R1/89C02F101/16
CPCC02F3/322C02F2101/105C02F2101/16C12N1/125C12R2001/89
Inventor 邓禹卢伟强张晓娟赵运英毛银
Owner JIANGNAN UNIV
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