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Rumen fungi with high plant cell wall degrading activity and their application in feed silage

A plant cell and fungus technology, applied in the preservation method of animal feed raw materials, animal feed, application, etc., can solve problems such as production that is not widely used, and achieve the effect of improving fermentation quality and improving degradation rate.

Active Publication Date: 2020-03-17
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, rumen fungi producing cell wall degrading enzymes have been screened from rumen and feces of ruminants such as yak (Bos grunniens) and beef cattle (Bos taurus) (Zhu et al., 1997, Anaerobe, 3(1):49-59; Gruninger et al., 2014, FEMS Microbiology Ecology, 90(1):1-17; Wei et al., 2016b, Journal of Applied Microbiology, 3(120):571-587), but they are not widely used in production

Method used

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  • Rumen fungi with high plant cell wall degrading activity and their application in feed silage
  • Rumen fungi with high plant cell wall degrading activity and their application in feed silage
  • Rumen fungi with high plant cell wall degrading activity and their application in feed silage

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Effect test

preparation example Construction

[0055] In addition, the embodiment of the present invention discloses a method for preparing a combined enzyme for plant cell wall degradation, including the steps of fermenting and culturing Piromyces sp.CN6 and purifying the enzyme in the fermentation broth.

[0056] The invention also discloses a method for preparing crop straw silage, which includes the step of adding Piromyces sp.CN6 bacterium or culture solution in the silage project.

[0057]In a preferred embodiment, add Piromyces sp.CN6 thalline 10 5 TFU / g or the number of fungi in the culture solution >10 6 TFU ml -1 .

[0058] In a preferred embodiment, the method for preparing silage further includes the step of fermenting and culturing Piromyces sp. CN6 before silage.

[0059] In a preferred embodiment, the crop stalks are corn stalks.

Embodiment 1

[0061] Example 1 Isolation and identification of rumen fungi with high plant cell wall degrading enzyme activity and analysis of enzymatic characteristics

[0062] 1.1 Materials and methods

[0063] The source of the strain: the rumen content was taken from the animal husbandry teaching base of Northwest Agriculture and Forestry University, three Xinong Saanen dairy goats equipped with permanent rumen fistula and in good body condition, the experimental animals were fed once at 08:30 and 17:30 respectively , the ratio of dietary concentrate to crude food was 3:7, free drinking water, dietary composition and nutritional level are shown in Table 1. Collection of rumen contents: 2 hours after morning feeding of dairy goats, the rumen contents are collected from the fistula through a negative pressure device, and filled with sterilized and filled with CO 2 Put the anaerobic bottle into a thermos bottle at 39°C and quickly bring it back to the laboratory for isolation and culture ...

Embodiment 2

[0107] Example 2 Effects of Piromyces sp.CN6 on the Fermentation Quality, Nutrients and In Vitro Digestibility of Whole Plant Corn Silage

[0108] 2.1 Materials and methods

[0109] Silage raw material: Whole-plant corn (Zhongyuan Dan 32) cultivated in the Animal Husbandry Teaching Base of Northwest A&F University, mowed at the milky stage, with a water content of about 64%, crushed to 1-3cm. The composition of the whole plant corn is shown in Table 4.

[0110] Table 4 Nutrient composition of whole corn

[0111]

[0112] Composite enzyme preparation: special additive for silage produced by Japan Snow Brand Seed Company, solid dosage form, containing cellulase and xylanase, the activities are 90FPU / g and 6000IU / g respectively.

[0113] Fungal inoculant: strain Piromyces sp.CN6 is a rumen anaerobic fungus with high plant cell wall degrading enzyme activity isolated from Xinong Shaneng dairy goats in the laboratory. 6 TFUml -1 .

[0114] The experiment adopted a single fa...

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Abstract

The invention discloses a rumen fungus with high plant cell wall degrading activity and its application in feed silage, belonging to the technical field of microorganisms and feed processing. The present invention adopts Hengate anaerobic rolling tube technology to separate and screen anaerobic fungi with cell wall degrading enzyme activity from the rumen of Xinong Sanen dairy goats, and finally screen to obtain xylanase and acetyl esterase at a wide temperature and pH Piromyces sp.CN6, which has high activity in the range and good thermal stability and acid-base stability, has a preservation number of CGMCC No.14449. This strain can improve the fermentation quality of silage and increase the degradation of crude fiber rate, it can be used as a silage additive in actual production.

Description

technical field [0001] The invention belongs to the technical field of microbes and feed processing, and in particular relates to the isolation and identification of a rumen fungus with high plant cell wall degrading enzyme activity and its application effect evaluation in silage. Background technique [0002] As an important feed material for ruminants, crop straw is an extremely abundant and renewable lignocellulosic biomass resource in China. The annual output of crop straw in my country reaches more than 840 million tons, but only 22.6-27.5% of the straw is used as feed, and more than 300 million tons of straw are not used, resulting in great waste. Crop straw silage and yellow silage are two effective ways of important high-quality roughage sources for dairy livestock breeding in my country. Adding cellulase-producing fungi or cellulase during silage can destroy plant cell walls, improve the nutritional quality of silage, reduce the crude fiber content in straw, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23K30/18C12R1/645
CPCA23K30/18C12N1/14C12R2001/645C12N1/145A23V2400/169
Inventor 曹阳春姚军虎王腊梅王砀砀蔡传江徐秀容张涛赵聪聪任豪
Owner NORTHWEST A & F UNIV
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