Method for increasing in-vivo early-embryo implantation quantity and litter size of female animal

Abstract

The invention relates to the field of animal breeding and particularly discloses a method for increasing an in-vivo early-embryo implantation quantity and litter size of a female animal and an application of N-acetylcysteine (NAC) in the aspect of increasing the in-vivo early-embryo implantation quantity and litter size of the female animal. According to the method, taking mice and pigs for example, the intake of the NAC aims at improving uterine environments and improving the developmental potency of early embryos, the quantity of implanted embryos is increased, and thus, the NAC has the capacity for increasing the litter size of female animals; and the NAC belongs to micromolecular compounds, is relatively stable in chemical properties and relatively low in production cost and has a broad application prospect and huge economic benefits.

Description

technical field [0001] The invention relates to the field of animal reproduction, in particular to a method for increasing the number of implanted early embryos and litter size in female animals. Background technique [0002] Superovulation (Superovulation, referred to as superovulation) is the application of exogenous gonadotropin to induce the development of multiple ovarian follicles, and to discharge eggs that are several times or even ten times more than those under natural conditions and have the ability to fertilize. Within the effective reproductive years, obtain their offspring as much as possible, rapidly expand the excellent population, or obtain more eggs for in vitro fertilization and early embryo culture. At present, superovulation mainly uses serum gonadotropin (PMSG) to stimulate follicular development, and then human chorionic gonadotropin-releasing hormone (hCG) or luteinizing hormone (LH) and gonadotropin-releasing hormone analog (GnRH- a) Induce ovulatio...

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Problems solved by technology

Therefore, in response to this problem, researchers tried to add a certain proportion of folic acid to the diet of superovulated sows, but did not increase the litter size of sows (Longenecker DE, Day BN. Fertility level of sowssuperovulated at post-weaning estrus.J Anim Sci 1968; 27:709-711.); although the literature showed that intraperitoneal injection of melatonin to female CD-1 mice without PMSG superovulation treatment would increase litter size (He C, Wang J, Li Y, Zhu K, Xu Z, Song Y, Song Y, Liu G. Melatonin-related genes expressed in the mouse uterus during early gestation promote embryo implantation. J Pineal Res 2015; 58:300-309.), but long-term use fades Melanin will affect its own endocrine, and even produce dependence

And because melatonin is easy to decompose when exposed to light, all operations need to be carried out in the dark, resulting in cumbersome processing; adding N-carbamylglutamic acid (NCG) to the diet can increase the litter size of rats and sows (ZhuJ, Zeng X, Peng Q, Zeng S, Zhao H, Shen H, Qiao S. Maternal N-Carbamylglutamate Supplementation during Early Pregnancy Enhances Embryonic Survival and Development through Modulation of the Endometrial Proteome in Gilts. J Nutr 2015; 145:2212-2220.), but its Whether it can increase the litter size of over-rowing dams, there is no relevant research for the time being; under the background of using PMSG to treat female animals with simultaneous estrus or timing insemination and the urgent need to improve the economic benefits of breeding enterprises and farmers, increase the litter size of dams is an inevitable trend

At present, there are few studies on the low implantation ability of early embryos after superovulation and no increase in litter size, and no satisfactory results have been achieved so far.

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