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SNP Molecular Markers Associated with Upland Cotton Fiber Length and Its Application

A molecular marker and fiber length technology, applied in the field of molecular biology, can solve the problems of long genetic distance between candidate genes and molecular markers, and complex molecular mechanism.

Active Publication Date: 2020-08-11
HEBEI AGRICULTURAL UNIV. +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researchers used molecular marker techniques such as AFLP and SSR to initially locate fiber length genes, but the genetic distance between candidate genes and molecular markers is generally far away, indicating that the molecular mechanism of cotton fiber length formation is very complex and needs to be further studied and explored. Excavating and utilizing these genes that control fiber length will enrich the genetic resources for improving fiber quality and provide an important basis for the cultivation of new cotton varieties that meet various needs

Method used

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  • SNP Molecular Markers Associated with Upland Cotton Fiber Length and Its Application
  • SNP Molecular Markers Associated with Upland Cotton Fiber Length and Its Application
  • SNP Molecular Markers Associated with Upland Cotton Fiber Length and Its Application

Examples

Experimental program
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Embodiment 1

[0073] Example 1 Acquisition of SNP molecular markers associated with upland cotton fiber length

[0074] 1. Determination of fiber length: 419 cotton core germplasm resources field experiments were carried out in Cangzhou, Hebei, Anyang, Henan, Jingzhou, Hubei, Yancheng, Jiangsu, Dunhuang, Gansu and Alar, Xinjiang for two consecutive years in 2014 and 2015 respectively. . Residential Area 6m 2 , 1 row per plot, row spacing 0.8m, plant spacing 0.3m, observation track 0.8m, isolation track 1.2m, 20 plants per repetition. The experiment was set to repeat 3 times, and the normal field cultivation management. During the peak cotton bolling period, 1-2 normal bolls were harvested on the first and second fruit nodes in the middle of each normal cotton plant, a total of 20. After ginning, the lint samples were tested for fiber quality by HVI 1000, and the data The collection refers to the description specification of cotton germplasm resources, and the phenotype values ​​of 12 env...

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Abstract

The invention provides a batch of SNP molecular markers associated with the length of upland cotton fibers. The nucleotide sequences of the SNP molecular markers are as shown in any one of the SEQ ID NO:1-1661, the 50th base of each sequence has the SNP mutation site, and at the site, the base is associated with the traits of the continuous fibers of the upland cotton. The SNP molecular markers provided by the invention can be used for the early predication and screening for the length trait of the cotton fibers, and also can be used for the breeding of the long-fiber cotton varieties.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a batch of SNP molecular markers associated with upland cotton fiber length and applications thereof. Background technique [0002] Cotton fiber is the most important part of the economic value of cotton and has an important economic position in the world textile industry. Fiber quality is a comprehensive index of fiber length, strength, fineness and other properties. With the development of the textile industry and the improvement of people's living standards, the requirements for the quality of cotton fibers are getting higher and higher. The quality of cotton fiber in my country is relatively poor, which is manifested by the mismatch between the main quality indicators, the single type, the fiber length is mostly concentrated in 27-29mm, and the spinnability is poor. Therefore, selecting long fiber quality that meets market demand is one of the main goals and research purpos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11C40B40/06
CPCC12Q1/6895C12Q2600/13C12Q2600/156C40B40/06
Inventor 马峙英王省芬杜雄明张艳张桂寅何守朴吴立强孙君灵吴金华李志坤杨君贾银华潘兆娥王国宁柯会锋
Owner HEBEI AGRICULTURAL UNIV.
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