SNP Molecular Markers Associated with Upland Cotton Fiber Length and Its Application
A molecular marker and fiber length technology, applied in the field of molecular biology, can solve the problems of long genetic distance between candidate genes and molecular markers, and complex molecular mechanism.
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[0073] Example 1 Acquisition of SNP molecular markers associated with upland cotton fiber length
[0074] 1. Determination of fiber length: 419 cotton core germplasm resources field experiments were carried out in Cangzhou, Hebei, Anyang, Henan, Jingzhou, Hubei, Yancheng, Jiangsu, Dunhuang, Gansu and Alar, Xinjiang for two consecutive years in 2014 and 2015 respectively. . Residential Area 6m 2 , 1 row per plot, row spacing 0.8m, plant spacing 0.3m, observation track 0.8m, isolation track 1.2m, 20 plants per repetition. The experiment was set to repeat 3 times, and the normal field cultivation management. During the peak cotton bolling period, 1-2 normal bolls were harvested on the first and second fruit nodes in the middle of each normal cotton plant, a total of 20. After ginning, the lint samples were tested for fiber quality by HVI 1000, and the data The collection refers to the description specification of cotton germplasm resources, and the phenotype values of 12 env...
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