Goat-friendly site SETD5-IN, sgRNA specifically targeting site, and coding DNA and application thereof

A SETD5-IN and friendly site technology, applied in the field of genetic engineering, can solve problems such as system off-target, reduced efficiency of gene-directed editing, and functional impact of off-target sites
CN113373152AActive Publication Date: 2021-09-10CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-09-10

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Abstract

The invention discloses a goat-friendly site SETD5-IN, sgRNA specifically targeting the site and a coding DNA and application thereof. The invention specifically discloses sgRNA for editing a goat-friendly site SETD5-IN (SEQ ID No.1), a DNA molecule for coding the sgRNA, a CRISPR / Cas9 targeting vector for editing the friendly site SETD5-IN, and a gene editing method. The goat-friendly site SETD5-IN of the invention can be transferred into an exogenous gene or a site-specific overexpression endogenous gene and the like, the expression of the SETD5 gene and an adjacent gene cannot be influenced, a new safe site is provided for gene site-specific integration, gene editing can be efficiently and specifically carried out by utilizing the sgRNA with high targeting efficiency and low off-target rate of the invention, and a foundation is laid for researching functions of important economic character related genes of livestock and producing transgenic animals.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a goat-friendly site SETD5-IN, a sgRNA specifically targeting the site, its coding DNA and applications. Background technique

[0002] In the process of producing transgenic animals, integrating the target gene into a specific site (target site) in the animal genome is a key step. When selecting the target, on the one hand, the transferred segment should be avoided to affect the normal physiological activities of the animal. Therefore, the target is usually located in the non-coding region of the genome. Rosa26 (Reverse orientation splice acceptorβ-geo 26) gene is a common insertion site for exogenous genes, and its transcription product is long non-coding RNA (long none coding RNA, lncRNA), which can be widely expressed in various tissues of the body, so many studies have included Rosa26 Genes are considered as friendly transgenic sites. However, with th...

Claims

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