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Group of siRNA molecules capable of effectively reducing PRDM1beta expression and application thereof

A PRDM1, molecular technology, applied in the direction of recombinant DNA technology, DNA / RNA fragments, medical preparations containing active ingredients, etc., can solve the problem of tumor cell insensitivity to chemotherapy drugs, to overcome drug resistance of lymphoma, improve treatment Efficacy, Enhancement, and Prognostic Effects

Inactive Publication Date: 2012-12-05
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

[0014] Since clinical studies have shown that PRDM1β may be one of the poor prognosis factors of lymphoma, its expression may cause tumor cells to be insensitive to chemotherapy drugs.

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  • Group of siRNA molecules capable of effectively reducing PRDM1beta expression and application thereof
  • Group of siRNA molecules capable of effectively reducing PRDM1beta expression and application thereof
  • Group of siRNA molecules capable of effectively reducing PRDM1beta expression and application thereof

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Embodiment Construction

[0039] The specific embodiments provided by the present invention will be described in detail below in conjunction with the accompanying drawings.

[0040] (1) Cell lines and cell culture

[0041] The multiple myeloma cell line 8226, the T-cell lymphoma cell line H9, and the B-cell lymphoma cell line Namalwa were all purchased from the American Standard Biological Collection (ATCC). All cells were cultured in 1640 medium containing 10% fetal bovine serum in 5% CO 2 -95% air, saturated humidity and cultured at 37°C. The survival rate of the cells was detected by the Moss blue exclusion test, and the cell morphology was observed by the Wright's staining method.

[0042] (2) Reagents

[0043] RNA extraction reagent Trizol and RNA reverse transcription kit were purchased from Invitrogen, pEGFP-N2Vector was purchased from BD Biosciences Clontech, pSlencer 4.1-H1 neo Vector was purchased from Ambion, KOD Plus high-fidelity DNA polymerase was purchased from TOYOBO, T-easy was pur...

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Abstract

The invention relates to a group of siRNA molecules capable of effectively reducing PRDM1beta expression and application thereof. Target spots of the siRNA molecules are GTTTGAGGCAGCTCCTTAA(66-84bp), GAGGCAGCTCCTTAAATGA(70-88bp) and GCAGCTCCTTAAATGAACA(73-91bp) respectively. The target spots of the siRNA molecules are positioned in a 5' untranslated region (5'UTR) of PRDM1beta mRNA. The PRDM1betaexpression in lymphoma cells can be effectively lowered by the siRNA molecules. After the PRDM1beta is reduced by the siRNA molecules, sensitivity of lymphoma cells to a chemotherapeutical medicamentadriamycin is improved. The siRNA molecules can reverse lymphoma-resistant medicaments, can improve curative effect on the lymphoma and can be applied to targeted treatment on the lymphoma.

Description

【Technical field】 [0001] The present invention relates to a group of siRNA molecules, in particular to a group of siRNA molecules capable of effectively down-regulating the expression of PRDM1β and applications thereof. 【Background technique】 [0002] Lymphoma is a malignant tumor that originates in lymph nodes or extranodal lymphoid tissue. In my country, the incidence of lymphoma is about 3-4 / 100,000, with about 45,000 new patients and more than 20,000 deaths every year. The incidence rate is the ninth among male malignant tumors and the tenth among female malignant tumors. bit. And the incidence rate is increasing year by year, seriously threatening human health. [0003] Lymphoma is divided into Hodgkin Lymphoma (Hodgkin Lymphoma, HL) and non-Hodgkin Lymphoma (Non-Hodgkin Lymphoma, NHL) two categories, of which NHL is the main one, accounting for about 80-90%. Lymphoma is often treated with a combination of chemotherapy, radiotherapy, immune-targeted therapy, and bone ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K31/704
Inventor 赵维莅陈赛娟张群岭杨帆
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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