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Pharmacy use of laetiporus sulphureus extract

A technology of sulfur bacteria and extracts, applied to the application field of medicine, can solve the problem that virus infection can not be blocked, hepatitis B virus can not solve the problem of adverse drug reaction and curative effect, etc., to achieve anti-virus recurrence, prevent recurrence, reduce effect of recurrence

Pending Publication Date: 2019-08-30
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even if the treatment of hepatitis B virus is carried out at the same time as anti-tumor treatment, it cannot solve the problems of adverse drug reactions and curative effects, let alone solve the problem of blocking the whole process from virus infection to liver cancer.

Method used

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  • Pharmacy use of laetiporus sulphureus extract
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  • Pharmacy use of laetiporus sulphureus extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of Sulfur Bacteria Extract, Trihydropinenic Acid B and Dental Acid

[0034] Experimental material used in the present invention

[0035] Laetiporus sulphureus (Fr.) Muttill was identified by Professor Wang Shaobo of Three Gorges Tourism Vocational and Technical College and provided fruiting bodies and strains.

[0036] Reagents used in the present invention

[0037] 95% medical alcohol, double distilled water, chromatographic grade acetonitrile, petroleum ether, acetone, etc.

[0038] Experimental instrument used in the present invention

[0039] KQ-500DB Ultrasonic Cleaner, Airon Rotary Evaporator (Germany), ME215S 1 / 100,000 Electronic Balance (Germany Sartorios Company), Ultraviolet Spectrophotometer (UV-2201, Japan Shimadzu Company), High Performance Liquid Phase Chromatography (DIONEX Ultimate 3000, Dionex Liquid Chromatography Co., Ltd., USA), NMR (UltrashiedTM400MHZ Plus, bruker Bruker), freeze dryer (FREEZONE PLUS 6, LABCONCO).

[0040] Preparatio...

Embodiment 2

[0049] Application of Sulfur Bacteria Extract, Trihydropinenic Acid B and Dental Acid as Anti-Hepatitis B Virus Drugs

[0050] Drugs and Cell Lines

[0051] The ethanol extract of sulfur bacteria, dentoic acid, and 3-hydropinemic acid B were provided by our laboratory. For cell experiments, use a solution of DMSO: absolute ethanol = 1:9 to dissolve the drug, filter through a 0.22 μm microporous water filter membrane, and finally make a drug with an initial mass concentration of 10 mg / mL, and then dilute it with the medium to reach the desired concentration Perform cell experiments.

[0052] Human liver cancer HepG2 / 2.2.15 cell line was purchased from Nanjing Kebai Biotechnology Co., Ltd.

[0053] Main reagent used in the process of the present invention

[0054] Human hepatitis B surface antigen (HBsAg) ELISA detection kit, product of Beijing Kewei Clinical Diagnostic Reagent Co., Ltd.;

[0055] q-PCR kit, Sangon Bioengineering (Shanghai) Co., Ltd. product;

[0056] Rever...

Embodiment 3

[0071] ELISA kit for detection of HBsAg secretion

[0072] HepG2 / 2.2.15 cells in the logarithmic growth phase were selected, digested with trypsin into a single cell suspension, 2×10 4 mL -1 Cells were inoculated at a concentration of 100 μL / well in a 96-well plate, placed at 37°C, 5% CO 2 Cultivate in an incubator for 12 hours, and then add MEM medium containing 0.0 (control), 2.5, 5, 10.0, 15.0 and 20.0 μg / mL of each drug respectively, and set 3 replicate wells for each concentration. After culturing for 24 and 48 hours, the supernatants of cells cultured for 24 and 48 hours were collected, respectively, and stored at -20°C. According to the instructions of the ELISA kit, the content of HBsAg in the supernatant was determined. Select a wavelength of 450nm, read the OD value of each well with a microplate reader, take the average value, and calculate the inhibition percentage.

[0073] After collecting part of the supernatant, add 20 μL of MTT with a concentration of 5 g / ...

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Abstract

The invention provides an application of a laetiporus sulphureus extract to preparation of a medicine for resisting hepatitis b virus. The laetiporus sulphureus extract is an extract obtained throughperforming refluxing on a laetiporus sulphureus fruiting body or mycelium with an alcohol solvent, wherein the alcohol solvent comprises ethanol, and the concentration of the ethanol is 95% and above.The laetiporus sulphureus and components thereof can prevent hepatitis b infection, treat hepatitis b infectious hepatitis, prevent liver cancer, treat hepatitis b relevance liver cancer, and preventrecurrence. The laetiporus sulphureus extract has positive effects in the overall process of pathological changes. The laetiporus sulphureus extract has important significance on solving the difficult problem of clinical treatment of hepatitis b infection and particularly solving the difficult problem of a hepatitis b relevance liver cancer technique.

Description

technical field [0001] The invention relates to a pharmaceutical use of a sulfur bacteria extract and its main components, in particular to the application of the sulfur bacteria extract and its main components in the preparation of anti-hepatitis B virus drugs. Background technique [0002] The human liver cancer HepG2 / 2.2.15 cell line is derived from the liver cancer cell line HepG2, which integrates two head-to-tail HBV complete genes in the HepG2 genome, and the cell line can stably and continuously express infectious HBV particles , and express HBV-related proteins HBsAg, HBeAg and HBcAg, etc., and produce a large number of viral replication intermediates. The mRNAs of HBV encode core antigen (HBcAg), envelope protein antigen (HBsAgs), polymerase (pol) and X protein (HBx), respectively. HBV has a double coat, and hepatitis B surface antigen (HBsAg) is the membrane protein of the HBV coat. The HBsAg envelope enters the endoplasmic reticulum after the hepatitis B virus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P31/20A61P1/16A61K31/575
CPCA61K35/74A61P31/20A61P1/16A61K31/575
Inventor 汪鋆植袁圆王新新王宇张宏岐苏香萍邹坤
Owner CHINA THREE GORGES UNIV
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