Four-base Microsatellite Genetic Marker Site and Its Screening Method in Forest Musk Deer

A technology of genetic markers and microsatellites, which is applied in the field of four-base microsatellite genetic marker sites and their screening in forest musk deer, can solve the problem that the number of microsatellite markers is less than 50, so as to improve development efficiency, reduce development costs, and successfully high rate effect

Active Publication Date: 2021-04-20
SICHUAN GOODDOCTOR PANXI PHARMA
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Problems solved by technology

However, the number of published microsatellite markers is less than 50, and all published forest musk microsatellite markers are dibasic microsatellites

Method used

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  • Four-base Microsatellite Genetic Marker Site and Its Screening Method in Forest Musk Deer
  • Four-base Microsatellite Genetic Marker Site and Its Screening Method in Forest Musk Deer
  • Four-base Microsatellite Genetic Marker Site and Its Screening Method in Forest Musk Deer

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Experimental program
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Embodiment Construction

[0031] 1 Materials and methods

[0032] 1.1 Experimental materials

[0033] 1.11 Specimen acquisition and DNA preparation

[0034] The 26 captive forest musk deer blood samples used for microsatellite marker screening in this chapter came from Miyaluo Farm in Sichuan Province and were stored at -20°C. The genomic DNA in the blood samples of forest musk deer was extracted using the kit and stored at -20°C.

[0035] 1.12 The source of the four-base microsatellite sequence of forest musk deer

[0036] The original sequence of the microsatellite primer design was derived from the shallow sequencing sequence of the forest musk deer genome.

[0037] 1.2 Main reagents and equipment

[0038] 1.21 Experimental main reagents

[0039] DNA extraction kit (Tiangen Biotechnology Company, Beijing), Taq enzyme, nucleic acid molecular weight standard DL2000, agarose and dNTPs were purchased from Qingke Biotechnology Co., Ltd.

[0040] 1.22 Main Experimental Instruments

[0041] (1) Cons...

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Abstract

The invention provides a four-base microsatellite genetic marker site for forest musk deer and a screening method thereof, and specifically relates to a screening method for polymorphic four-base microsatellite genetic markers in the forest musk deer genome and 17 four-base microsatellite genetic markers with medium and high polymorphisms. Satellite marker features. The present invention uses the bioinformatics method to search the four-base microsatellite sequence in the whole genome of the forest musk deer, designs primers, and undergoes steps such as primer PCR condition optimization, multicolor fluorescent labeling, genotyping scanning, etc., and screens to obtain 17 medium-high polymorphisms Four-base microsatellite genetic marker loci. The method has the advantages of quick experiment, easy operation, high success rate and cost saving.

Description

technical field [0001] The invention belongs to the fields of biotechnology and genetics, and in particular relates to four-base microsatellite genetic marker sites of forest musk deer and a screening method thereof. Background technique [0002] Wood musk musk deer (Moschus berezovskii) belongs to the order Artiodactyla, musk deer musk deer. The musk secreted by the adult male musk musk glands has high economic value and medicinal value. Driven by huge economic interests, wild hunting is rampant, coupled with the continuous destruction of its habitat, the population of forest musk deer has declined sharply, and the existing musk deer is endangered. In order to effectively protect the forest musk deer, unremitting efforts have been made at home and abroad. While actively protecting the wild forest musk deer, the artificial breeding of the forest musk deer is encouraged. The captive musk deer once reached 3,000 in the 1980s. Although the artificial breeding of forest musk d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 刘越飞岳碧松耿介
Owner SICHUAN GOODDOCTOR PANXI PHARMA
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