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Application of LED-blue light source having wavelength of 450-490 nm in colon cancer treatment

A technology for colon cancer and colon cancer cells, applied in the field of photomedicine, can solve problems such as surgical trauma, achieve the effects of inhibiting cell proliferation and migration, low medical costs, and remission and treatment of colon cancer

Inactive Publication Date: 2017-11-21
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some defects in the existing treatment methods, such as irreversible trauma caused by surgery.

Method used

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  • Application of LED-blue light source having wavelength of 450-490 nm in colon cancer treatment
  • Application of LED-blue light source having wavelength of 450-490 nm in colon cancer treatment
  • Application of LED-blue light source having wavelength of 450-490 nm in colon cancer treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: 450-490nm LED-blue light illumination inhibits the growth of colon cancer cells

[0025] 1 Experimental method

[0026] 1.1 Experimental grouping design:

[0027] The experiment was divided into the following five groups according to the light intensity of 450-490nm LED-blue light: 0J / cm 2 , 72J / cm 2 , 144J / cm 2 , 216J / cm 2 and 288J / cm 2 . The same number of SW620 colon cancer cells and HT29 colon cancer cells were irradiated respectively.

[0028] 1.2 Trypan blue staining to detect the survival rate of colon cancer cells

[0029] After 24 hours of light, trypan blue staining was performed, and the cells were counted.

[0030] 1.3. Data processing

[0031] The results of the present invention are represented by standard deviation±standard error. Graphpad prism 5.0 was used for graphing, and the correlation between each group was measured by T test.

[0032] 2 Observations

[0033] 2.1 450-490nm LED-blue light inhibits the growth of SW620 cells

...

Embodiment 2

[0038] Example 2: 450-490nm LED-blue light illumination inhibits colon cancer cell migration

[0039] 1 Experimental method

[0040] 1.1 Experimental grouping design:

[0041] The experiment is divided into 2 groups: 450-490nm LED-blue light illumination 0J / cm 2 Group and 450-490nm LED-blue light illumination 144J / cm 2 Group, respectively irradiated the same number of SW620 colon cancer cells and HT29 colon cancer cells, observed and photographed under the microscope 24h, 48h and 72h after irradiation to record the migration of the cells.

[0042] 1.2 Scratch test to detect the migration ability of colon cancer cells

[0043] Use a 200 μl pipette tip to scratch the surface of SW620 colon cancer cells and HT29 colon cancer cells to keep the width of the scratches as consistent as possible. After 24h, 48h and 72h of light, the cells were observed under a microscope and photographed to record cell migration.

[0044] 1.3. Data processing

[0045]The results of the present in...

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PUM

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Abstract

The invention discloses an application of LED-blue light source having a wavelength of 450-490 nm in colon cancer treatment and belongs to the field of photomedicine. According to the invention, tests prove that the LED-blue light having wavelength of 450-490 nm can inhibit colon cancer cell proliferation and thus an aim of relieving and treating colon cancer can be achieved. The clinical application is simple and easy and the medical cost is low. At the same time, patient pain is relieved. The invention provides the novel technical means for treating colon cancer.

Description

technical field [0001] The invention relates to the application of a 450-490nm wavelength LED-blue light source in the treatment of colon cancer. The invention belongs to the technical field of photomedicine. Background technique [0002] Colon cancer is a malignant tumor of the digestive tract. It is one of the common malignant tumors that seriously endanger human health, and its incidence is increasing year by year. Most patients are found to be in the middle and late stages. At present, the clinical treatment of colon cancer is mainly based on surgical resection of the tumor, and at the same time, it is supplemented with radiotherapy and chemotherapy, targeted therapy, and gene therapy. With the improvement of medical level, the development of colon cancer treatment field is changing with each passing day. However, there are still certain defects in the existing treatment methods, such as irreversible trauma caused by surgery. Therefore, there is an urgent need for a s...

Claims

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Application Information

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IPC IPC(8): A61N5/06
CPCA61N5/06A61N2005/065A61N2005/0662
Inventor 杨宝峰杜智敏蔡本志袁野
Owner HARBIN MEDICAL UNIVERSITY