Method for measuring free hemoglobin in blood bag based on double-light-path within-frequency-domain fluorescence intensities

Abstract

The invention discloses a method for measuring free hemoglobin in a blood bag based on double-light-path within-frequency-domain fluorescence intensities. The method comprises the following steps that a square signal drives a fluorescent excitation light source, the emergent light outlet of the fluorescent excitation light source and the incident slit of a light intensity receiving device both cling to the blood bag and are coaxial, the fluorescent excitation light source excites a blood sample to generate fluorescent light, and the light intensity receiving device receives a fluorescence intensity; a displacement platform controls the fluorescent excitation light source to move on the premise that the emergent light outlet of the fluorescent excitation light source and the incident slit of the light intensity receiving device are ensured to be coaxial, and the light intensity receiving device receives another fluorescence intensity; the two acquired fluorescence intensities are respectively converted to a frequency domain for construction of within-frequency-domain fluorescence intensities, then normalization is carried out, and a mathematical model is constructed after normalization on the basis of chemical examination data; the fluorescence intensities of an unknown blood sample at two positions are collected and respectively converted to the frequency domain for construction of within-frequency-domain fluorescence intensities, then normalization is carried out, and the normalized fluorescence intensities are substituted into the mathematical model for calculation of the content of free hemoglobin. The method provided by the invention greatly inhibits non-linear influence caused by self absorption of fluorescent light.

Description

technical field [0001] The invention relates to the field of stoichiometry for concentration analysis of complex solutions with light intensity, in particular to a method for measuring free hemoglobin in blood bags with fluorescent light intensity in a dual-path frequency domain. Background technique [0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which cannot meet the needs of fast, fast and efficient testing. Convenience, non-contact, and pollution-free requirements, in view of this problem, the present invention proposes a method for measuring free hemoglobin in a blood bag by fluorescent light intensity in a dual optical path frequency domain. Contents of the invention [0003] The present invention provides a method for...

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Problems solved by technology

[0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which cannot meet the needs of fast, fast and efficient testing. Convenience, non-contact, and pollution-free requirements, in response to this problem, the present invention proposes a method for measuring free hemoglobin in blood bags by fluorescent light intensity in the frequency domain with dual optical paths

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