Method for measuring free hemoglobin in blood bag based on double-light-path within-frequency-domain fluorescence intensities

A technology of hemoglobin and fluorescent light, which is applied in the direction of fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems that cannot meet the requirements of fast, simple, non-contact, and pollution-free, and achieve pollution-free high-precision measurement, Measuring the effectiveness of targeted, background noise cancellation

Inactive Publication Date: 2017-11-21
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which ca

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  • Method for measuring free hemoglobin in blood bag based on double-light-path within-frequency-domain fluorescence intensities
  • Method for measuring free hemoglobin in blood bag based on double-light-path within-frequency-domain fluorescence intensities
  • Method for measuring free hemoglobin in blood bag based on double-light-path within-frequency-domain fluorescence intensities

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Embodiment 1

[0056] The embodiment of the present invention provides a method for measuring free hemoglobin in a blood bag by fluorescent light intensity in the dual optical path frequency domain. The devices used are such as figure 2 As shown, it includes: a fluorescent excitation light source 3 , a blood bag 5 , a displacement platform 6 and a light intensity receiving device 7 .

[0057] Wherein, ensure that the light outlet of the fluorescence excitation light source 3 is close to the blood bag 5 and coaxial with the incident slit of the light intensity receiving device 7, and drive the fluorescence excitation light source 1 with a square wave signal to make it emit a square wave light signal. A position a (corresponding to the first optical path 1) excites the blood sample in the blood bag 5, and the light intensity receiving device 7 receives the fluorescence light intensity. Subsequently, the displacement platform 6 controls the fluorescence excitation light source to move to the s...

Embodiment 2

[0064] The difference between the embodiment of the present invention and the embodiment 1 is only that the fluorescence excitation light source 3 and the movement mode of the light intensity receiving device 7 are different, see the following description for details:

[0065] see image 3 , ensure that the light outlet of the fluorescence excitation light source 3 and the incident slit of the light intensity receiving device 7 are close to the blood bag 5 and coaxial, drive the fluorescence excitation light source 1 with a square wave signal to make it emit a square wave light signal, and the fluorescence excitation light source 3 pairs with the blood bag The blood sample in 5 is excited, and the fluorescent light intensity is received by the light intensity receiving device 7 at the first position a. Then, the displacement platform 6 is used to control the light intensity receiving device 7 to move to the second position b under the premise of ensuring that the light outlet ...

Embodiment 3

[0070] The difference between the embodiment of the present invention and embodiment 1 is only that the fluorescent excitation light source 3 and the moving direction of the light intensity receiving device 7 are different, see the following description for details:

[0071] see Figure 4 , ensure that the fluorescence excitation light source 3 and the light intensity receiving device 7 are close to the blood bag 5 and ensure that the light outlet of the fluorescence excitation light source 3 and the incident slit of the light intensity receiving device 7 are coaxial, and drive the fluorescence excitation light source 1 with a square wave signal to emit The square wave light signal is used to excite the blood sample in the blood bag 5 by the fluorescence excitation light source 3 at the first position a, and the fluorescence light intensity is received by the light intensity receiving device 7 at the first position a', and then passed through the displacement platform 6 at Und...

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Abstract

The invention discloses a method for measuring free hemoglobin in a blood bag based on double-light-path within-frequency-domain fluorescence intensities. The method comprises the following steps that a square signal drives a fluorescent excitation light source, the emergent light outlet of the fluorescent excitation light source and the incident slit of a light intensity receiving device both cling to the blood bag and are coaxial, the fluorescent excitation light source excites a blood sample to generate fluorescent light, and the light intensity receiving device receives a fluorescence intensity; a displacement platform controls the fluorescent excitation light source to move on the premise that the emergent light outlet of the fluorescent excitation light source and the incident slit of the light intensity receiving device are ensured to be coaxial, and the light intensity receiving device receives another fluorescence intensity; the two acquired fluorescence intensities are respectively converted to a frequency domain for construction of within-frequency-domain fluorescence intensities, then normalization is carried out, and a mathematical model is constructed after normalization on the basis of chemical examination data; the fluorescence intensities of an unknown blood sample at two positions are collected and respectively converted to the frequency domain for construction of within-frequency-domain fluorescence intensities, then normalization is carried out, and the normalized fluorescence intensities are substituted into the mathematical model for calculation of the content of free hemoglobin. The method provided by the invention greatly inhibits non-linear influence caused by self absorption of fluorescent light.

Description

technical field [0001] The invention relates to the field of stoichiometry for concentration analysis of complex solutions with light intensity, in particular to a method for measuring free hemoglobin in blood bags with fluorescent light intensity in a dual-path frequency domain. Background technique [0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which cannot meet the needs of fast, fast and efficient testing. Convenience, non-contact, and pollution-free requirements, in view of this problem, the present invention proposes a method for measuring free hemoglobin in a blood bag by fluorescent light intensity in a dual optical path frequency domain. Contents of the invention [0003] The present invention provides a method for...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486G01N2021/6419G01N2201/06113G01N2201/08
Inventor 李刚张梦秋万兴华倪静林凌
Owner TIANJIN UNIV
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