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Method of analyzing glycated protein, analysis reagent, analysis kit, and test piece for analysis

A technology of glycosylated protein and analysis method, which is applied in the direction of material analysis through observation of the influence of chemical indicators, analysis of materials through chemical reactions, and analysis of materials, etc. It can solve complicated operations, turbidity of reaction liquid, troublesome cost, etc. problem, to achieve the effect of simple and accurate analysis

Active Publication Date: 2017-11-21
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the first method, two reaction systems (two reaction systems) using different reagents are required for the sample, which is troublesome and costly.
In addition, in the case of the second method, the use of uricase causes the reaction solution to become cloudy, which affects the measurement of absorbance. In addition, in the use of the above-mentioned glycosaminoglycan as an insoluble carrier, it needs to be immobilized. , the operation becomes complicated due to cleaning, reaction stop, etc.

Method used

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  • Method of analyzing glycated protein, analysis reagent, analysis kit, and test piece for analysis
  • Method of analyzing glycated protein, analysis reagent, analysis kit, and test piece for analysis
  • Method of analyzing glycated protein, analysis reagent, analysis kit, and test piece for analysis

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Experimental program
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Embodiment 1

[0120] With regard to the analysis method of glycated protein of the present invention, it was confirmed that the concentration of glycated protein can be measured without being affected by uric acid, which is a reducing substance in blood, by measuring fructosamine.

[0121] (1) Sample

[0122] Serum 1 (uric acid not added-) with a fructosamine concentration of 198.5 μmol / L and serum 2 (uric acid not added-) with a fructosamine concentration of 503 μmol / L were prepared, and the serum 1 and serum 2 were prepared at 20 mg / 100 mL Serum 1 (uric acid added +) and serum 2 (uric acid added +) added with uric acid were used as serum samples.

[0123] (2) Production of analytical reagent sheets

[0124] Using the first liquid reagent and the second liquid reagent of the following composition, the above-mentioned figure 1 The test piece for analysis of the example shown.

[0125]

[0126]

[0127]

[0128]

[0129]

[0130] 1.5 μL of the above-mentioned second reagent w...

Embodiment 2

[0147] In the method for analyzing glycated protein of the present invention, it was confirmed by measuring fructosamine that the concentration of glycated protein can be measured without being affected by uric acid, which is a reducing substance in blood, at various concentrations of WST-9.

[0148] As Example 2, the concentration of the chromogenic agent WST-9 in the above-mentioned second liquid reagent is respectively set to 1mmol / L, 2.5mmol / L, 5mmol / L, 7.5mmol / L, 10mmol / L and 20mmol / L , except that, in the same manner as in Example 1 (2) above, six types of analytical test pieces 1 having different concentrations of WST-9 were produced. In addition, as serum samples, serum 4 (uric acid not added-) having a fructosamine concentration of 220.3 μmol / L and serum 5 (uric acid not added-) having a fructosamine concentration of 486.5 μmol / L were prepared, and the serum 4 and the above serum Among 5, serum 4 (uric acid supplemented +) and serum 5 (uric acid supplemented +) supple...

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Abstract

The invention relates to a method of analyzing glycated protein, an analysis reagent, an analysis kit, and a test piece for analysis. An object of the disclosure is to provide a method by which a glycated protein in a biological sample can be analyzed more simply and accurately. A method of analyzing a glycated protein in a biological sample, the method comprising: reacting a color developer represented by the following Formula (1) with a biological sample; and measuring a color reaction of the color developer, wherein, in Formula (1), X is an alkali metal ion.

Description

technical field [0001] The present invention relates to a method for analyzing glycated protein, an analysis reagent, an analysis kit and a test piece for analysis. Background technique [0002] Glycated protein in blood is used as an index of short-term blood sugar control. Glucose aldehyde groups are bonded to the N-terminal or amino groups of the side chains of proteins in the blood, and amadori rearrangement produces glycated proteins that are Amadori compounds. The resulting glycated protein is also known as fructosamine due to the fructose structure of the side chain. Fructosamine is a general term for glycated proteins present in the blood. [0003] Glycated protein is a reducing substance, so a substrate that develops color by reacting with a reducing substance (such as 2-(4-indolephenyl)-3-(4-nitrophenyl)-5-phenyl-2H -Tetrazolium chloride (INT) etc.) to measure glycated protein by the degree of color development of the matrix. However, the above-mentioned matrix...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N33/523G01N33/66G01N33/525G01N33/68G01N33/52
Inventor 中村勤滨隆志
Owner ARKRAY INC