Botanical pesticide chenopodium ambrosioides essential oil for purpose of preventing and curing tea geometrid
A technology of Nepeta mustard essential oil and botanical pesticides, applied in the field of pesticide application, can solve the problems of increased risk, increased dosage, unfavorable environmental and ecological safety, etc., and achieves the effects of reducing the amount of pesticides used, reducing environmental pollution, and reducing residues.
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example 1
[0019] Bioassay Example 1 : Effects of Nepeta essential oil on the hatching rate of first-laid eggs of Tea Geometrid
[0020] Test object: tea inchworm
[0021] Specific test method:
[0022] 1) Accurately weigh the Nepeta fruit, use the steam distillation method, and extract continuously for 2 hours to obtain a light yellow oily liquid with a strong aroma, namely the essential oil of Nepeta nepeta. The extracted Nepeta essential oil was stored in a refrigerator at 4°C for later use.
[0023] 2) Determination of the effect of fumigation on the inhibition of hatching of the first eggs of the tea geometrid. Spread qualitative filter paper with a diameter of 90 mm in a plastic petri dish with a diameter of 90 mm, and properly spray water to keep it moist. The first eggs of tea geometrid were picked and placed in petri dishes (30 heads / dish).
[0024] 3) Then place a sterilized circular piece of filter paper with a diameter of 10 mm in the petri dish. Add a certain dose of N...
example 2
[0030] Bioassay Example 2 : Indoor Toxicity Determination of Nepeta Essential Oil and Osthole Composition to Tea Geometrid
[0031] Test object: tea inchworm
[0032] The test method is leaf soaking method. Immerse the fresh tea tips in the liquid medicine and dry the cuttings, cut off the dried tea tips and put them in canned bottles, with 3-4 tea tips per bottle, select 2nd instar larvae of the same size and put them into the canned bottle. Each treatment was inoculated with 15 worms, repeated three times, the temperature was controlled at 26-27°C, and the appropriate humidity was maintained, and normal fresh leaves were fed. The mortality rate was investigated 120 hours after administration, and the inhibitory intermediate concentration LC50 was calculated by the least square method, and the co-toxicity coefficient (CTC) was calculated by Sun Yunpei's method. When CTC120, the composition exhibits synergistic effect.
Embodiment 1
[0043] Weigh 16g of nepeta essential oil, 0.8g of osthole, 12g of agricultural milk 500#, 8g of agricultural milk 1601#, and 150# solvent oil to 100g. The above raw materials are put into a mixing tank according to the conventional method for preparing cream and mixed to make active ingredient weight percentage of 16.8% Nepeta essential oil · osthole cream.
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