Shrimp and crab water-rich tissue paraffin section manufacturing method

Abstract

The invention relates to a shrimp and crab water-rich tissue paraffin section manufacturing method. A common paraffin tissue section method is lack of tissue distinction degree in tissue and organs of water-rich shrimps and crabs difficult to have texture fixed and soft in texture and vulnerable to tissue fragmentation, overlapping and warpage resulting in serious affect on microscopic observation effect of the tissue and difficulty in obtaining of an ideal and clear tissue structure. In the method, through negative pressure, fixation and embedding of a sample, the tissue is shaped and extended to avoid error brought by fold overlapping and elasticity of the water-rich tissue. The method is convenient in operation, highly practicable and short in cycle, good tissue distinction degree is achieved for the tissue organs of the water-rich shrimps and crabs difficult to have texture fixed and soft in texture, paraffin section observation with a complete tissue form can be realized, and all requirements of research personnel on section tissue observation can be met.

Description

technical field [0001] The invention relates to a method for preparing paraffin slices of water-rich tissues of shrimps and crabs, and belongs to the technical field of microscopic tissue slices. Background technique: [0002] The paraffin section technique of shrimp and crab tissue is an important technique for studying animal tissue morphology and pathological changes. Since shrimp and crabs are aquatic animals, the water content of various tissues and organs is relatively high, especially the gill tissue and hepatopancreas (midgut gland) tissue that are often used as the basis for pathological research. In recent years, the amount of materials and reagents used in the research of the above two tissues of shrimps and crabs is large. Due to economic considerations, traditional techniques are still widely used. The technique generally includes the steps of material collection and fixation, washing with running water, tissue dehydration, tissue transparency, paraffin embeddi...

AI Technical Summary

Problems solved by technology

However, conventional method indicators lack tissue differentiation for shrimp and crab tissues and organs that are difficult to fix in tissue shape, soft in texture, and rich in water, which is easy to cause tissue fragmentation, overlapping, and bending, which seriously affects the microscopic observation of the tissue, and it is difficult to obtain ideal clarity. organizational structure

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