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Surface plasmon resonance sensor chip for detecting estradiol in aqueous phase and preparation method thereof

A surface plasmon and resonance sensor technology, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of high response value and low response value, and achieve the effect of simple operation steps

Inactive Publication Date: 2020-02-14
BEIJING INSTITUTE OF TECHNOLOGYGY
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Problems solved by technology

This method realizes the detection of estradiol molecules in the aqueous phase, but its response value is high in acidic environment and low in neutral water environment

Method used

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  • Surface plasmon resonance sensor chip for detecting estradiol in aqueous phase and preparation method thereof
  • Surface plasmon resonance sensor chip for detecting estradiol in aqueous phase and preparation method thereof
  • Surface plasmon resonance sensor chip for detecting estradiol in aqueous phase and preparation method thereof

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Embodiment 1

[0035] A surface plasmon resonance sensor chip for detecting estradiol in aqueous phase, such as figure 1 As shown, it is composed of glass substrate 1, gold film layer 2, mercaptoethylamine monolayer 3, chain transfer reagent molecular layer 4 coupled to mercaptoethylamine and molecularly imprinted film 5; the mercaptoethylamine monolayer The end of the molecular layer 3 is an amino group, and the end of the molecular layer 4 of the chain transfer reagent coupled to mercaptoethylamine is a carboxyl group, which is coupled through a chemical coupling method through dicyclohexylcarbodiimide.

[0036] A method for preparing a surface plasmon resonance sensor chip for detecting estradiol in an aqueous phase, the specific steps are as follows:

[0037] (1) Glass substrate 1 cleaning

[0038] Put a 2.5×30cm LASFN9 glass substrate 1 into a vertical staining vat, ultrasonically clean it with 2% detergent water for 15 minutes, then ultrasonically soak it in double-distilled water for...

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Abstract

The invention relates to a surface plasmon resonance sensor chip for water phase detection of estrodiol and a preparation method of the chip, and belongs to the field of surface plasmon resonance sensor chips. The surface plasmon resonance sensor chip consists of a glass substrate, a gold film layer, a self-assembly monomolecular layer, a chain transfer reagent molecular layer and a molecularly imprinted polymer film layer, wherein the chain transfer reagent molecular layer is formed by combining a chain transfer reagent and the self-assembly monomolecular layer by a coupling reagent; and the molecularly imprinted polymer film layer is formed by grafting on the surface of the chain transfer reagent molecular layer by utilizing ultraviolet light initiation. The method effectively solves the problem of weak binding force of a molecularly imprinted polymer film to a target molecule in a water environment; no additional photoinitiator or catalyst is added in a preparation process of the chip; the preparation process is simple and controllable; and the chip is stable in chemical property, good in selectivity, easy to store and reusable.

Description

technical field [0001] The invention relates to a surface plasmon resonance sensor chip for detecting estradiol in water phase and a preparation method thereof, belonging to the surface plasmon resonance sensor (Surf ace Plasmon Resonance, referred to as SPR) chip field. Background technique [0002] Estradiol is a steroidal estrogen and an important sex hormone that can promote and regulate the normal development of female sexual organs and secondary sexual characteristics. Detection of estradiol levels has important clinical significance for diagnosing female precocious puberty and assisting in judging and determining the time of ovulation. At present, there are many methods and equipment for detecting estradiol, such as liquid chromatography Environ.Sci.Technol.46 (2012) 7128-7134, gas chromatography with Anal.Bioanal.Chem.397 (2010) 1335-1345, ELISA J.Enviro.Manage.124(2013)121-127 and other methods. These methods are characterized by accuracy and high sensitivity, b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/552
CPCG01N21/554
Inventor 韦天新王洋
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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