Method for aided detection of cattle growth characters through copy number variation (CNV) markers on KLF3genes and kit special for aided detection of cattle growth characters through copy number variation (CNV) markers on KLF3genes

A technology for growth traits and auxiliary detection, applied in the field of molecular genetics, can solve problems that have not been seen yet, and achieve the effect of accurate copy number variation type and low cost

Inactive Publication Date: 2017-11-28
NORTHWEST A & F UNIV
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Problems solved by technology

[0006] But so far, there is no report on the method of real-time quantitative PCR (qPCR) of genomic DNA to detect the variation of bovine Kruppel-like transcription factor 3 (Kruppel-like factors3, KLF3) gene copy number

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  • Method for aided detection of cattle growth characters through copy number variation (CNV) markers on KLF3genes and kit special for aided detection of cattle growth characters through copy number variation (CNV) markers on KLF3genes
  • Method for aided detection of cattle growth characters through copy number variation (CNV) markers on KLF3genes and kit special for aided detection of cattle growth characters through copy number variation (CNV) markers on KLF3genes
  • Method for aided detection of cattle growth characters through copy number variation (CNV) markers on KLF3genes and kit special for aided detection of cattle growth characters through copy number variation (CNV) markers on KLF3genes

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Embodiment Construction

[0031] The invention will be described in detail below in conjunction with the accompanying drawings and embodiments, and the embodiments are to explain rather than limit the present invention.

[0032] In the previous cattle genome resequencing study, a copy number variation located in the 8201bp-9600bp region of the KLF3 gene reference sequence was found. Therefore, the present invention designs specific primers based on the region of copy number variation in the cattle KLF3 gene sequence obtained by resequencing, and then uses the genomic DNA of cattle (Qinchuan, Pinan, Xianan, Nanyang, Qaidam cattle) as a template, Perform qPCR amplification, and use the BTF3 gene as an internal reference gene, using 2 -ΔΔCt A method for determining the type of copy number variation of an individual. Based on the physiological function of the KLF3 gene and the regulatory mechanism of CNV, the relationship between the copy number variation of the KLF3 gene and growth traits was studied to ...

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Abstract

The invention discloses a method for aided detection of cattle growth characters through copy number variation (CNV) markers on a KLF3genes and a kit special for aided detection of the cattle growth characters through the copy number variation (CNV) markers on the KLF3genes. Based on the real-time fluorescent quantitative PCR technology, cattle genome DNA is used as a template, CAN areas of the cattle KLF3genes are amplified, cattle basic transcription factor 3 (BTF3) genes are amplified as the internal reference, and finally the CAN type of individuals is calculated and judged through the 2<-delta delta Ct> method. The method for aided detection of the cattle growth characters through the copy number variation (CNV) markers on the KLF3genes lays a foundation for building the relevance between the KLF3genes CNV and the growth characters of cattle, cattle molecule mark-assist selection breeding work is accelerated advantageously, and the method is simple, rapid and convenient to apply and popularize.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and specifically relates to a method for detecting the copy number variation of bovine KLF3 gene. The method utilizes real-time quantitative PCR of genomic DNA, takes BTF3 gene as a reference, and determines the type of copy number variation of an individual according to the -ΔΔCt value. Realize molecular marker-assisted detection of cattle growth traits. Background technique [0002] Genomic DNA, as the carrier of biological genetic information, plays a vital role in the formation of individual phenotypes, while genomic variation is the main genetic source of inter-individual or inter-species differences. How to effectively and accurately detect and excavate these key loci related to phenotypic traits is a hot spot and problem to be solved urgently in modern genetics research. [0003] Molecular breeding is molecular mark-assisted selection (MAS), which is a technology that uses DNA molecular ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q1/6888C12Q2600/124C12Q2600/156C12Q2537/16C12Q2561/113
Inventor 黄永震徐嘉威郑立张静贺花文逸凡张子敬曹修凯宋成创雷初朝陈宏
Owner NORTHWEST A & F UNIV
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