Cereal grain processing
A technology of grains and grains, applied in the field of regulating grains, capable of solving difficulties, changing operating systems, etc.
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example 1
[0263] In this example, two different enzyme compositions were used: (i) hydrolyzing β-glucan and non-starch polysaccharides (eg with β-glucanase activity of 1500-1700 AZO BBG U / g and 6200-7580 IU / g The enzyme composition (herein referred to as "cellulase / β-glucanase complex") of the cellulase activity of T. reesei fermenting arabinoxylan produced by T. reesei); and (ii) activity 180000 units / g of bacterial xylanase.
[0264] Trials with the wheat variety KERUBINO resulted in significantly higher extraction yields through reduced conditioning time. The result is as figure 2 shown.
[0265] When the extraction rate is increased, it is important that the flour quality does not change significantly so that the flour quality remains the same. Slightly higher ash levels were sometimes measured, but had no effect on baking performance or flour color as shown in Table 2. The results were confirmed in several trials and Table 1 shows the results for American durum wheat.
[0266...
example 2
[0280] A. Cell Wall Lysis Assay :
[0281] In some embodiments, bran solubility can be measured using the following assay.
[0282] A suspension of wheat bran (0.1M) in disodium hydrogen phosphate (0.2M) buffer (pH 5.0) was prepared as a 1.33% bran (w / w) concentrate. Aliquots of 750 μl were transferred from this suspension to Eppendorf tubes with stirring. Each substrate tube was preheated at 40°C for 5 minutes. 250 µl of enzyme solution was added thereto so that the final concentration of the substrate was 1%. For each assay (0, 30, 60 and 240 minutes), three dilutions (in duplicate) were prepared from each enzyme composition according to the invention with increasing enzyme concentrations (for example 0.33, 1.0 and 3.0 μg enzyme / gram bran). As a blank, a heat-denatured solution of the enzyme composition was used. Reactions were terminated at the given times by transferring the tubes to an incubator set at 95°C. Heat denatured sample...
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