Method of promoting gene expression of CYP43 gene of siraitia grosvenorii

A technology of gene expression and Luo Han Guo, applied in the field of plant biology, to achieve the effect of unifying harvesting management, promoting concentrated expression, and increasing content

Inactive Publication Date: 2017-12-05
黄小华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on promoting the expression of the CYP43 gene in Luo Han Guo to increase the content of glucoside V in Luo Han Guo

Method used

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  • Method of promoting gene expression of CYP43 gene of siraitia grosvenorii
  • Method of promoting gene expression of CYP43 gene of siraitia grosvenorii

Examples

Experimental program
Comparison scheme
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example 1

[0035] Configure induction medium: According to the recipe: MS+1mg / L BA+0.1mg / L 2,4–D+2% sucrose+0.7% agar+1.0g / L activated carbon+50μmol / L methyl jasmonate, configure induction culture base

[0036] Induction culture: inoculate the tissue culture seedlings on the induction medium, maintain a constant temperature of 25°C, and a relative humidity of 70% to 80%, using artificial light, 12 hours of light and 12 hours of darkness per day, and cultivate for 35 days at a light intensity of 1000 to 1500 lux .

[0037]Transplant planting: soak and sterilize the induced tissue culture seedlings with 0.1% potassium permanganate solution; transplant the sterilized tissue culture plantlets into a sterilized medium, wherein the medium consists of 20 parts by weight of vermiculite, 15 parts by weight of perlite, 10 parts by weight of sticky sand, 15 parts by weight of chaff, 10 parts by weight of sawdust, and 10 parts by weight of mushroom slag; after transplanting, spray 10 mg / kg of ABT r...

example 2

[0040] Configure induction medium: According to the formula: MS+1mg / L BA+0.1mg / L 2,4–D+2% sucrose+0.7% agar+1.0g / L activated carbon+400μmol / L methyl jasmonate, configure induction culture base

[0041] Induction culture: inoculate the tissue culture seedlings on the induction medium, maintain a constant temperature of 25°C, and a relative humidity of 70% to 80%, using artificial light, 12 hours of light and 12 hours of darkness per day, and cultivate for 35 days at a light intensity of 1000 to 1500 lux .

[0042] Transplant planting: soak and sterilize the induced tissue culture seedlings with 0.1% potassium permanganate solution; transplant the sterilized tissue culture plantlets into a sterilized medium, wherein the medium consists of 20 parts by weight of vermiculite, 15 parts by weight of perlite, 10 parts by weight of sticky sand, 15 parts by weight of chaff, 10 parts by weight of sawdust, and 10 parts by weight of mushroom slag; after transplanting, spray 10 mg / kg of AB...

example 3

[0045] Configure induction medium: According to the recipe: MS+1mg / L BA+0.1mg / L 2,4–D+2% sucrose+0.7% agar+1.0g / L activated carbon+200μmol / L methyl jasmonate, configure induction culture base

[0046] Induction culture: inoculate the tissue culture seedlings on the induction medium, maintain a constant temperature of 25°C, and a relative humidity of 70% to 80%, using artificial light, 12 hours of light and 12 hours of darkness per day, and cultivate for 35 days at a light intensity of 1000 to 1500 lux .

[0047] Transplant planting: soak and sterilize the induced tissue culture seedlings with 0.1% potassium permanganate solution; transplant the sterilized tissue culture plantlets into a sterilized medium, wherein the medium consists of 20 parts by weight of vermiculite, 15 parts by weight of perlite, 10 parts by weight of sticky sand, 15 parts by weight of chaff, 10 parts by weight of sawdust, and 10 parts by weight of mushroom slag; after transplanting, spray 10 mg / kg of ABT...

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Abstract

The invention provides a method of promoting gene expression of a CYP43 gene of siraitia grosvenorii. The method comprises inoculating tissue culture seedlings of siraitia grosvenorii into an induction cultivation medium for induction cultivation; performing trickle irrigation on the roots of the transplanted tissue culture seedlings of the siraitia grosvenorii by a first induction liquid; and spraying a second induction liquid after flowering and pollination of the plants, wherein a certain concentration of methyl jasmonate is added in every step. The method provided by the invention has an effect that the gene expression of the CYP43 gene of the siraitia grosvenorii is promoted, so that accumulation of mogroside V content is promoted.

Description

technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for promoting the expression of CYP43 gene in Luo Han Guo. Background technique [0002] Luo Han Guo is a precious medicinal and sweet plant unique to my country. It is a perennial vine of the genus Siraitia in the family Cucurbitaceae. Its main component, glucoside V, is one of the strongest non-sugar sweeteners in the world, which is 300-400 times sweeter than sucrose. It is a low-calorie, pure natural sweetener and an ideal health product. It has anti-oxidation, immune regulation, anti-cancer, hypoglycemic and other effects. [0003] Sweet glycoside V cannot be chemically synthesized at present, and it is mainly obtained by extraction. Luo Han Guo has strict requirements on the habitat and is only suitable for growing in Guangxi, and it cannot be continuously cropped in cultivation. The content of glucoside V in the fruit is low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01H1/02A01N43/90A01N37/42A01N39/04A01P21/00
CPCA01H4/001A01G24/00A01G24/23A01H1/02A01H4/005A01N37/42A01N39/04A01N43/90A01N2300/00
Inventor 黄小华韦荣昌
Owner 黄小华
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