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Method for promoting expression of CYP24 gene of momordica grosvenori

A technology of gene expression and Luo Han Guo is applied in the field of plant biology to achieve the effects of increasing content, promoting concentrated expression, and facilitating unified harvesting management

Inactive Publication Date: 2017-10-10
黄小华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on promoting the expression of the CYP24 gene in Luo Han Guo to increase the content of glucoside V in Luo Han Guo

Method used

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  • Method for promoting expression of CYP24 gene of momordica grosvenori
  • Method for promoting expression of CYP24 gene of momordica grosvenori

Examples

Experimental program
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Effect test

example 1

[0036]Configure the induction medium: according to the formula: MS+0.5mg / L 6-BA+0.1mg / L IBA+3.5g / L agar+30g / l sucrose+1.0g / L activated carbon to configure the basal medium, add methyl jasmonate The methyl jasmonate mother solution prepared by dissolving 2% ethanol aqueous solution (v / v) into 10000 μmol / L is filtered and sterilized by a nitrocellulose membrane with a pore size of 0.20 μm, and the sterilized mother solution is added to the base In the culture medium, methyl jasmonate is made to reach the desired concentration, wherein the concentration of methyl jasmonate in the induction medium 1 is 50 μmol / L, and the concentration of methyl jasmonate in the induction medium 2 is 100 μmol / L;

[0037] Tissue culture: Tissue culture the young leaves of high-quality Luo Han Guo plants, cut the young leaves into leaf discs with a diameter of 1 cm, soak them in an aqueous solution of washing powder for 3 minutes, rinse them with distilled water, and place them on an ultra-clean workb...

example 2

[0041] Configure the induction medium: according to the formula: MS+0.5mg / L 6-BA+0.1mg / L IBA+3.5g / L agar+30g / l sucrose+1.0g / L activated carbon to configure the basal medium, add methyl jasmonate The methyl jasmonate mother solution prepared by dissolving 2% ethanol aqueous solution (v / v) into 10000 μmol / L is filtered and sterilized by a nitrocellulose membrane with a pore size of 0.20 μm, and the sterilized mother solution is added to the base In the culture medium, methyl jasmonate is made to reach the desired concentration, wherein the concentration of methyl jasmonate in the induction medium 1 is 400 μmol / L, and the concentration of methyl jasmonate in the induction medium 2 is 500 μmol / L;

[0042] Tissue culture: Tissue culture the young leaves of high-quality Luo Han Guo plants, cut the young leaves into leaf discs with a diameter of 1 cm, soak them in an aqueous solution of washing powder for 3 minutes, rinse them with distilled water, and place them on an ultra-clean wor...

example 3

[0046] Configure the induction medium: according to the formula: MS+0.5mg / L 6-BA+0.1mg / L IBA+3.5g / L agar+30g / l sucrose+1.0g / L activated carbon to configure the basal medium, add methyl jasmonate The methyl jasmonate mother solution prepared by dissolving 2% ethanol aqueous solution (v / v) into 10000 μmol / L is filtered and sterilized by a nitrocellulose membrane with a pore size of 0.20 μm, and the sterilized mother solution is added to the base In the culture medium, methyl jasmonate is made to reach the desired concentration, wherein the concentration of methyl jasmonate in the induction medium 1 is 200 μmol / L, and the concentration of methyl jasmonate in the induction medium 2 is 300 μmol / L;

[0047] Tissue culture: Tissue culture the young leaves of high-quality Luo Han Guo plants, cut the young leaves into leaf discs with a diameter of 1 cm, soak them in an aqueous solution of washing powder for 3 minutes, rinse them with distilled water, and place them on an ultra-clean wor...

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Abstract

The invention provides a method for promoting the expression of a CYP24 gene of momordica grosvenori. The method comprises the step of adding an induction factor of jasmonic acid methyl ester in the processes of culture and plantation of momordica grosvenori. By virtue of the method, the expression of the CYP24 gene of the momordica grosvenori is effectively promoted, so that the accumulation of the content of mogroside V is promoted.

Description

technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for promoting the expression of CYP24 gene in Luo Han Guo. Background technique [0002] Luo Han Guo is a precious medicinal and sweet plant unique to my country. It is a perennial vine of the genus Siraitia in the family Cucurbitaceae. Its main component, glucoside V, is one of the strongest non-sugar sweeteners in the world, which is 300-400 times sweeter than sucrose. It is a low-calorie, pure natural sweetener and an ideal health product. It has anti-oxidation, immune regulation, anti-cancer, hypoglycemic and other effects. [0003] Sweet glycoside V cannot be chemically synthesized at present, and it is mainly obtained by extraction. Luo Han Guo has strict requirements on the habitat and is only suitable for growing in Guangxi, and it cannot be continuously cropped in cultivation. The content of glucoside V in the fruit is low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02
CPCA01H4/005A01H1/02A01H4/001
Inventor 黄小华韦荣昌
Owner 黄小华
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