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Method for promoting momordica grosvenori CYP4 gene expression

A technology of gene expression and Luo Han Guo, applied in the field of plant biology, to achieve the effect of promoting concentrated expression, facilitating unified picking management, and increasing content

Inactive Publication Date: 2017-10-27
韦荣昌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on promoting the expression of CYP4 gene in Luo Han Guo to increase the content of sweet glycoside V in Luo Han Guo

Method used

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  • Method for promoting momordica grosvenori CYP4 gene expression
  • Method for promoting momordica grosvenori CYP4 gene expression
  • Method for promoting momordica grosvenori CYP4 gene expression

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] Tissue culture the callus of high-quality Luo Han Guo in an induction medium to obtain tissue culture plantlets of Luo Han Guo, wherein the concentration of 2,4-epibrassinolide in the induction medium is 50 μmol / L;

[0032] Wherein, the induction medium also includes a basal medium, and its formula is: MS+0.5mg / L BA+0.1mg / LIBA+3.5g / L agar+30g / l sucrose+1.0g / L activated carbon+10μmol / L iron salt.

[0033] Wherein, the induction culture conditions are: constant temperature of 25° C., relative humidity of 70% to 80%, artificial light, 12 hours of light and 12 hours of darkness per day, wherein the light intensity is 1000 to 1500 lux.

[0034]The preparation method of the induction medium is as follows: 2,4-epibrassinolide is dissolved in 2% ethanol aqueous solution (v / v), prepared into a 10000 μmol / L mother solution, and sterilized with a 0.22 μm microporous membrane, Add the sterilized 2,4-epibrassinolide mother solution to the prepared basal medium that is still in liq...

example 2

[0037] Tissue culture the callus of high-quality Luo Han Guo in an induction medium to obtain tissue culture plantlets of Luo Han Guo, in which the concentration of 2,4-epibrassinolide in the induction medium is 400 μmol / L;

[0038] Wherein, the induction medium also includes a basal medium, and its formula is: MS+0.5mg / L BA+0.1mg / LIBA+3.5g / L agar+30g / l sucrose+1.0g / L activated carbon+10μmol / L iron salt.

[0039] Wherein, the induction culture conditions are: constant temperature of 25°C, relative humidity of 70%-80%, artificial light, 12 hours of light and 12 hours of dark culture per day, wherein the light intensity is 1000-1500 lux.

[0040] The preparation method of the induction medium is as follows: 2,4-epibrassinolide is dissolved in 2% ethanol aqueous solution (v / v), prepared into a 10000 μmol / L mother solution, and sterilized with a 0.22 μm microporous membrane, Add the sterilized 2,4-epibrassinolide mother solution to the prepared basal medium that is still in liqu...

example 3

[0043] Tissue culture the callus of high-quality Luo Han Guo in the induction medium to obtain the tissue culture plantlet of Luo Han Guo, the concentration of 2,4-epibrassinolide in the induction medium is 200 μmol / L;

[0044] Wherein, the induction medium also includes a basal medium, and its formula is: MS+0.5mg / L BA+0.1mg / LIBA+3.5g / L agar+30g / l sucrose+1.0g / L activated carbon+10μmol / L iron salt.

[0045] Wherein, the induction culture conditions are: constant temperature of 25° C., relative humidity of 70% to 80%, artificial light, 12 hours of light per day, 12 hours of dark culture, light intensity of 1000 to 1500 lux.

[0046] The preparation method of the induction medium is as follows: 2,4-epibrassinolide is dissolved in 2% ethanol aqueous solution (v / v), prepared into a 10000 μmol / L mother solution, and sterilized with a 0.22 μm microporous membrane, Add the sterilized 2,4-epibrassinolide mother solution to the prepared basal medium that is still in liquid state so ...

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Abstract

The invention provides a method for promoting momordica grosvenori CYP4 gene expression. The method comprises the steps of carrying out tissue culture on calluses of high-quality momordica grosvenori in an induction medium; and obtaining tissue culture seedlings of momordica grosvenori and carrying out special treatment on pollen after plants bloom to obtain a pollen liquid, wherein 2,4-epibrassinolide is added to the process. The method is capable of promoting momordica grosvenori CYP4 gene expression, thereby playing a role in promoting content accumulation of mogroside V.

Description

technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for promoting the expression of CYP4 gene of Luo Han Guo. Background technique [0002] Luo Han Guo is a precious medicinal and sweet plant unique to my country. It is a perennial vine of the genus Siraitia in the family Cucurbitaceae. Its main component, glucoside V, is one of the strongest non-sugar sweeteners in the world, which is 300-400 times sweeter than sucrose. It is a low-calorie, pure natural sweetener and an ideal health product. It has anti-oxidation, immune regulation, anti-cancer, hypoglycemic and other effects. [0003] Sweet glycoside V cannot be chemically synthesized at present, and it is mainly obtained by extraction. Luo Han Guo has strict requirements on the habitat and is only suitable for growing in Guangxi, and it cannot be continuously cropped in cultivation. The content of glucoside V in the fruit is low,...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/02A01G7/06C05G3/00A01N59/00A01N43/22A01N43/16A01N39/04A01P21/00
CPCA01G7/06A01H1/02A01H4/00A01H4/001A01N39/04A01N43/16A01N43/22A01N59/00C05C11/00C05G3/00C05G5/23A01N2300/00C05D9/02C05F11/00C05F11/10
Inventor 韦荣昌黄小华
Owner 韦荣昌
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