Partial pancreas decellularized bioscaffold applicable to circulatory docking transplantation, preparation method and application thereof

Abstract

The invention discloses a partial pancreas decellularized biological scaffold applied for circular butting transplant, a preparation method and an application thereof. The biological scaffold is prepared by using the tail part of a corpus pancreatis as the main body, using a celiac trunk artery as a perfusion inlet passage, and using a splenic venin trunk or a portal venin as a perfusion outlet passage, through the decellularization. The preparation method comprises the following steps: 1, preparing the partial pancreas needed by the scaffold model, namely determining an acquisition mode for the corpus pancreatis tail part and the inlet passage and the outlet passage of the decellularization perfusion; 2, a detailed process and specific perfusion liquid of the decellularization; and 3, a specific mode of the biological scaffold applied for the in-vivo circular butting transplant. The related decellularized biological scaffold is capable of completely removing cells, and integrally keeping a vessel structure and an epimatrix component. The pancreas biological scaffold has the unique independent artery inlet passage and the vein outlet passage. The impermeability is good, and the biological scaffold has the unique application value of the in-vivo circular butting transplant.

Description

Technical field [0001] The invention relates to tissue engineering and regenerative medicine research, in particular to a partial pancreas decellularized biological scaffold that can be applied to cyclic docking transplantation, a preparation method and application thereof. Background technique [0002] Organ transplantation is the gold standard treatment for all diseases that cause irreversible failure of organ function. However, we are facing a serious shortage of donors. Tissue engineering and regenerative medicine research replace or partially replace normal organ functions through the reconstruction of functional tissues or organs, thus playing an unprecedented role in clinical organ transplantation. One of its core technologies is the organ decellularization perfusion technology, which removes the cellular components inside the organ to obtain a biological scaffold structure that retains the natural vascular structure and matrix components, which contributes to the differen...

AI Technical Summary

Problems solved by technology

At present, the treatment of type 1 diabetes mainly relies on long-term insulin injections, but it cannot be completely relieved, nor can it delay the multi-organ function involvement complicated by diabetes

Research in the field of pancreatic regeneration and tissue engineering based on the application of pancreatic decellularized bioscaffolds is an innovative way to construct a transplantable pancreas, which provides encouraging hope for the complete cure of diabetes in the future. However, due to anatomical The multiple arterial blood supply of the pancreas organ and the tight envelopment and extensive communication branches between the head of the pancreas and the duodenum make the existing whole pancreas decellularized bioscaffold models have the following two serious defects: 1. The access and exit vessels of the graft are not clear; 2. The stent lacks good circulatory sealing

The ultimate goal of tissue engineering and regenerative medicine research is to construct transplantable organs / tissues before they can be applied clinically. Therefore, the existing whole pancreas bioscaffold model is not the best application carrier for pancreatic tissue engineering research.

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