Application of serum 48-lncRNA as a diagnostic marker for chronic liver diseases
A chronic disease and liver technology, applied in the field of diagnostic targets for chronic liver diseases, can solve the problems of limited accuracy in the diagnosis and differential diagnosis of chronic liver diseases, and the lack of clinical application.
Active Publication Date: 2020-06-16
王辉云
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[0004] However, the reported signatures composed of single or a few lncRNAs have limited accuracy in the diagnosis and differential diagnosis of chronic liver diseases and cannot meet the requirements of clinical application.
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[0022] The inventor first extracted 109 serum samples from the training group (including 23 normal (HC) people, 30 chronic hepatitis B (CHB) patients, 30 liver cirrhosis (LC) patients, and 26 liver cancer (HCC) patients) 181 samples were selected between normal people and patients (including patients with chronic hepatitis B, liver cirrhosis and liver cancer) through chip scanning, data extraction, and data standardization processing. lncRNAs whose expression fold change is greater than 1.5 and p<0.001. Next, the inventors used Fisher discriminant analysis (stepwise regression method) to screen the 181 differentially expressed lncRNAs, and signed a set of molecular labels composed of 48 lncRNAs. The results showed that this label can 100% distinguish between normal and chronic B Hepatitis, cirrhosis and liver cancer.
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The invention discloses applications of a serum 48-lncRNA marker in serving as a target for diagnosing chronic diseases of the liver. The inventor extracts RNA of 109 serum samples of a training group, lncRNA expression profile chip hybridization is carried out, after chip scanning, data extraction and data standard processing, SAM analysis is carried out, and 181 lncRNAs with the expression change multiple being greater than 1.5 and p being smaller than 0.001 between normal people and patients (including patients suffering from chronic hepatitis B, liver cirrhosis and liver cancer) are selected. Then, the inventor screens the 181 lncRNAs with differential expression by adopting Fisher discriminant analysis, and then identifies one group of molecular tags composed of 48 lncRNAs, and the result shows that the tags can distinguish the normality, chronic hepatitis B, liver cirrhosis and liver cancer with 100 percent.
Description
technical field [0001] The present invention relates to a diagnostic target for chronic liver diseases, in particular to the application of serum lncRNA markers as diagnostic targets for primary hepatocellular carcinoma, liver cirrhosis and chronic hepatitis B. Background technique [0002] According to statistics, there were 782,500 new liver cancer cases and 745,500 liver cancer deaths worldwide in 2012, and about 50% of these new and dead liver cancer patients occurred in China. Studies have shown that hepatitis B virus infection is the main pathogenic factor of chronic hepatitis, liver cirrhosis and liver cancer. There are more than 240 million chronic hepatitis B patients in the world, some of whom will develop liver cancer. In China, hepatitis B virus carriers account for 10% of the total population, and the annual incidence of liver cancer in chronic hepatitis B patients without cirrhosis is 0.01-1.4%, while the incidence of liver cancer in chronic hepatitis B patien...
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IPC IPC(8): C12Q1/6886C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q1/6886C12Q2600/158C12Q2600/178
Inventor 王辉云曾丽斯张美殷
Owner 王辉云