Method for extracting seaweed protein by use of specially-made extraction kettle material bag

A technology for extracting seaweed and extraction kettle, which is applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of material wrapping into agglomerates, increased contact area, low extraction rate, etc., and achieves increased contact area. , Increase the direct contact area, the effect of easy access to the source

Inactive Publication Date: 2017-12-15
SOUTH CHINA UNIV OF TECH
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  • Application Information

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Problems solved by technology

The method uses a special extraction kettle material bag to extract seaweed protein, which increases the contact area between the material and the extract, and solves the problem of material wrapping and agglomeration that are easily caused by direct use of subcritical water extraction equipment in the prior art. , leading to the problem of low extraction rate, to achieve the active substance of seaweed protein with high extraction yield

Method used

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  • Method for extracting seaweed protein by use of specially-made extraction kettle material bag
  • Method for extracting seaweed protein by use of specially-made extraction kettle material bag
  • Method for extracting seaweed protein by use of specially-made extraction kettle material bag

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Embodiment 1

[0068] Using the above-mentioned special extraction kettle material bag, using subcritical water extraction equipment to prepare high-pressure water to extract protein from spirulina, the specific steps are as follows:

[0069] (1) First check whether the subcritical water extraction equipment can start normally, then add the water in the water storage device to the predetermined position of 15L, and start to prepare for the experiment. Amount 50g;

[0070] Spread the dry powder of spirulina evenly in the gauze layer of the 6-layer bracket hoop, put the bracket hoop into the gauze bag, close the gauze bag with a tie rope and hang it in the extraction kettle, cover the kettle, and insert the temperature sensor It is convenient to detect the temperature of the top and bottom of the extraction kettle and the temperature inside the kettle;

[0071] (2) After turning on the main switch of the equipment, turn on the switch of the water pump, control the flow rate of 100mL / min to in...

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Abstract

The invention discloses a method for extracting seaweed protein by use of a specially-made extraction kettle material bag. The method comprises steps as follows: (1) in subcritical water extraction equipment, seaweed meal is put in the specially-made extraction kettle material bag, the specially-made extraction kettle material bag is suspended in an extraction kettle, well preheated deoxidized and deionized water is injected into the extraction kettle, and heating and pressurization are performed for an extraction reaction under the ultrasonic condition; (2) after extraction ends, an extraction solution cooled to the room temperature is collected, cooling, precipitation and centrifugation are performed, a liquid supernatant is taken, frozen and dried, and crude seaweed protein is obtained. The specially-made extraction kettle material bag is used for extraction of the seaweed protein, the contact area of the extraction solution and a material is increased, the yield of the crude seaweed protein is notably increased, and the yield of the crude seaweed protein is increased by 4 times without ultrasonic assistance; and the yield of the crude seaweed protein is increased by 1.6 times under ultrasonic assistance.

Description

technical field [0001] The invention relates to the technical field of subcritical water extraction, in particular to a method for extracting seaweed protein by using a special material bag of an extraction kettle. Background technique [0002] Subcritical water, also known as high-pressure hot water, super hot water or hot liquid water, refers to high-temperature water with a temperature above 100°C and a critical temperature below 374°C under a certain pressure (generally 5-20MPa), and it has been kept liquid state. Subcritical water extraction is to use subcritical water as the extraction agent, in a closed, oxygen-free, high-pressure container, according to the principle of similar solubility of organic matter, through the molecular diffusion process of the extraction material and the extraction agent during the soaking process, to achieve the solid material It is a new extraction and separation technology that transfers the fat-soluble components in the liquid to the l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 范晓丹胡双飞张学武苏可盈
Owner SOUTH CHINA UNIV OF TECH
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