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Full-automatic digestion equipment for microcarrier and animal cell separation

A fully automatic technology for animal cells, applied in biochemical equipment and methods, biochemical cleaning devices, enzymology/microbiology devices, etc. Lock and other problems, to achieve high-efficiency separation, increase the effect of culture volume

Inactive Publication Date: 2017-12-15
黄山市中新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the microcarrier / bioreactor system, trypsin digestion amplification process, the digestion and transfer operation process is cumbersome, and the digestion process is fully manual without automatic control
Moreover, during the digestion process, the cells on the microcarriers are easily affected by various factors, resulting in a decrease in cell viability, growth rate and other indicators.
It is difficult to meet the requirements of the scale-up culture process of industrialized microcarrier / bioreactor system

Method used

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  • Full-automatic digestion equipment for microcarrier and animal cell separation
  • Full-automatic digestion equipment for microcarrier and animal cell separation
  • Full-automatic digestion equipment for microcarrier and animal cell separation

Examples

Experimental program
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Effect test

Embodiment 1

[0029] combine figure 1 As shown, in the embodiment of the present invention, a fully automatic digestion device for separating microcarriers from animal cells includes a motor 1, a separation component A16, a separation component B18, a tank body 20, a tank body top cover 10, and a controller 26.

[0030] The top of the tank body 20 is sealed and connected to the top cover 10 of the tank body. The separation part A16 and the separation part B18 are installed inside the tank body 20 and fixed on the pipeline A2 and pipeline C4 respectively. The pipeline A2 and pipeline C4 run through the top of the tank body. Cover 10; the tank top cover 10 also runs through the installation pipeline D5, pipeline F7, pipeline G8, and pipeline J12.

[0031] A motor motor 1 is installed on the tank top cover 10, and the rotating shaft of the motor motor 1 is connected to the stirring shaft inside the tank body 20 through a shaft seal device; a pipeline K13 is installed at the bottom of the tank...

Embodiment 2

[0059] combine figure 1 As shown, in the embodiment of the present invention, a fully automatic digestion device for separating microcarriers and animal cells, the digestion device includes: a tank body 20 , a separation unit A16 , a separation unit B18 and a controller 26 . The same as the equipment in Embodiment 1, the difference is:

[0060] The cell culture liquid in the tank body 20 is discharged through the pump D24, and when there is no liquid in the tank body 20, the pump D24 stops working. Turn on the pump A21 in the controller 26, and add the cell culture solution into the tank body 20 through the pipeline G8. When the supplement reaches 70% of the total volume of the tank body 20, the pump B21 stops working. The motor 1 keeps the stirring speed of the stirring paddle 17 at 80-100 rpm, so as to ensure that the cells can fall off from the surface of the microcarrier without causing damage to the cells. After waiting 2-3 minutes. Open the pipeline K13, and transfer ...

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Abstract

The invention discloses full-automatic digestion equipment for microcarrier and animal cell separation. The equipment comprises a motor, a separating part A, a separating part B, a tank body, a tank body top cover and a controller. The tank body top cover is hermetically connected to the top of the tank body, and the separating part A and the separating part B are located in the tank body and are mounted on the tank body top cover; the motor is mounted on the tank body top cover, and a rotary shaft of the motor is connected to a stirring shaft in the tank body through a shaft sealing device; the controller is electrically connected to the motor and pipelines. The full-automatic digestion equipment disclosed by the invention can separate microcarriers from anchorage-dependent cells efficiently when used in biological pharmaceutical industry, so that the defect of an existing animal cell digestion process is overcome, an a screen plate in a separating part can be prevented from being blocked by microcarriers and free cells in the cell digestion process.

Description

technical field [0001] The invention relates to the technical field of bioengineering devices, in particular to a fully automatic digestion device for separating microcarriers and animal cells. Background technique [0002] Microcarrier culture technique (microcarrier culture technique): Microcarriers use fine particles as cell carriers, which are suspended in the culture medium by stirring, so that cells can propagate into a single layer on the surface of the carrier. After half a century of development, this technology has been widely used and widely used in the production of biological products, such as vaccines, protein drugs, antibodies, etc. [0003] In the microcarrier / bioreactor system, obtaining a large number of suspended and free seed cells is a key technology for scale-up culture between bioreactors. From small-volume seed-type bioreactors to large-volume production-type bioreactors, there are trypsin digestion methods, ball transfer methods, low temperature and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12M1/02C12M1/00
CPCC12M21/18C12M23/48C12M27/02C12M29/00C12M37/04C12M41/34C12M47/04
Inventor 毕军李爱东古磊
Owner 黄山市中新生物科技有限公司